LOCUS BC073962 1229 bp mRNA linear HUM 09-JUN-2008
DEFINITION Homo sapiens neuronal guanine nucleotide exchange factor, mRNA
(cDNA clone IMAGE:6472601), partial cds.
ACCESSION BC073962
VERSION BC073962.1
KEYWORDS .
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1229)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 1229)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (23-JUN-2004) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC
cDNA Library Preparation: Life Technologies, Inc.
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: National Institutes of Health Intramural
Sequencing Center (NISC),
Gaithersburg, Maryland;
Web site: http://www.nisc.nih.gov/
Contact: nisc_mgc@nhgri.nih.gov
Akhter,N., Ayele,K., Beckstrom-Sternberg,S.M., Benjamin,B.,
Blakesley,R.W., Bouffard,G.G., Breen,K., Brinkley,C., Brooks,S.,
Dietrich,N.L., Granite,S., Guan,X., Gupta,J., Haghighi,P.,
Hansen,N., Ho,S.-L., Karlins,E., Kwong,P., Laric,P., Legaspi,R.,
Maduro,Q.L., Masiello,C., Maskeri,B., Mastrian,S.D.,McCloskey,J.C.,
McDowell,J., Pearson,R., Stantripop,S., Thomas,P.J., Touchman,J.W.,
Tsurgeon,C., Vogt,J.L., Walker,M.A., Wetherby,K.D., Wiggins,L.,
Young,A., Zhang,L.-H. and Green,E.D.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 174 Row: o Column: 17.
FEATURES Location/Qualifiers
source 1..1229
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="IMAGE:6472601"
/tissue_type="Uterus, leiomyosarcoma"
/clone_lib="NIH_MGC_71"
/lab_host="DH10B"
/note="Vector: pCMV-SPORT6"
gene <1..1229
/gene="NGEF"
/gene_synonym="EPHEXIN"
/db_xref="GeneID:25791"
/db_xref="HGNC:HGNC:7807"
/db_xref="MIM:605991"
CDS <1..872
/gene="NGEF"
/gene_synonym="EPHEXIN"
/codon_start=3
/product="NGEF protein"
/protein_id="AAH73962.1"
/db_xref="GeneID:25791"
/db_xref="HGNC:HGNC:7807"
/db_xref="MIM:605991"
/translation="SARQRWRRRLQLRSSRARCDPAPQPASRPRPSPGPRRGPRPGPR
TRPAADPMELLAAAFSAACAVDHDSSTSESDARDSAAGHLPGSESSSTPGNGATPEEW
PALADSPTTLTEALRMIHPIPADSWRNLIEQIGLLYQEYRDKSTLQEIETRRQQDAEI
EDNTNGSPASEDTPEEEEEEEEEEEPASPPERKTLPQICLLSNPHSRFNLWQDLPEIR
SSGVLEILQPEEIKLQEAMFELVTSEASYYKSLNLLVSHFMENERIRKILHPSEAHIL
FSNVLDVLAVSER"
BASE COUNT 342 a 361 c 313 g 213 t
ORIGIN
1 ggagtgctcg gcagcggtgg cggcggcggc tgcagctccg gtcgagccgc gcgcgctgcg
61 accccgctcc gcagcccgct agtcgccccc gacccagccc agggccccgg cgtggccccc
121 gacccggccc ccgcacccgc cccgccgccg accctatgga gctgctggcc gctgccttca
181 gcgccgcctg cgccgtggac cacgacagtt ccacctcgga aagcgacgcg cgcgactcgg
241 cggcgggaca cctgcccggc agtgagtcgt cctccacccc gggaaatggg gccacgcccg
301 aggagtggcc ggccctggcc gacagcccca ccacgctcac cgaggccctg cggatgatcc
361 accccattcc cgccgactcc tggagaaacc tcattgaaca aatagggctc ctgtatcagg
421 aataccgaga taaatcgact ctccaagaaa tcgaaaccag gaggcaacag gatgcagaaa
481 tagaagacaa taccaatggg tccccggcca gtgaggacac cccggaggag gaagaagaag
541 aggaggagga ggaggagccg gccagcccac cagagaggaa gactctgccc cagatctgcc
601 tgctcagtaa cccccactca aggttcaacc tctggcagga tcttcccgag atccggagca
661 gcggggtgct tgagatccta cagcccgagg agattaagct gcaggaggcc atgttcgagc
721 tggtcacttc cgaggcgtcc tactacaaga gtctgaacct gctcgtgtcc cacttcatgg
781 agaacgagcg gataaggaag atcctgcacc cgtccgaggc gcacatcctc ttctccaacg
841 tcctggacgt gctggctgtc agtgagcggt gaggacgggg gggacagcca cctccctcag
901 ggcccttctt catccccagg gcacatgctt gagacactga tgacaagtgc tcgatactgg
961 cttctctact tgcagatgtt tggaatgttc cataataaaa tataaataaa tgaagaacta
1021 aaaaaaaaaa aaaagggcgg ccgccctttt tttttttttt tttttttttt tttttttcat
1081 acaaccctga gcatttgcta cattcctttt tctattaaat atgtaaacga caataaaaca
1141 gttttgactt gaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
1201 aaaaaaaaaa aaaaaaaaaa aaaaaaaaa
//