LOCUS BC073752 1394 bp mRNA linear HUM 01-SEP-2006
DEFINITION Homo sapiens eukaryotic translation initiation factor 4A, isoform
1, mRNA (cDNA clone MGC:88756 IMAGE:6371531), complete cds.
ACCESSION BC073752
VERSION BC073752.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1394)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 1394)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (23-JUN-2004) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: DCTD/DTP/Gazdar
cDNA Library Preparation: Rubin Laboratory
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Sequencing Group at the Stanford Human Genome
Center, Stanford University School of Medicine, Stanford, CA 94305
Web site: http://www-shgc.stanford.edu
Contact: (Dickson, Mark) mcd@paxil.stanford.edu
Dickson, M., Schmutz, J., Grimwood, J., Rodriquez, A., and Myers,
R. M.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 58 Row: m Column: 3
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 4503528.
FEATURES Location/Qualifiers
source 1..1394
/db_xref="H-InvDB:HIT000264980"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:88756 IMAGE:6371531"
/tissue_type="Lung, large cell carcinoma"
/clone_lib="NIH_MGC_18"
/lab_host="DH10B-R"
/note="Vector: pOTB7"
gene 1..1394
/gene="EIF4A1"
/gene_synonym="DDX2A"
/gene_synonym="EIF-4A"
/db_xref="GeneID:1973"
/db_xref="HGNC:HGNC:3282"
/db_xref="MIM:602641"
CDS 4..1224
/gene="EIF4A1"
/gene_synonym="DDX2A"
/gene_synonym="EIF-4A"
/codon_start=1
/product="eukaryotic translation initiation factor 4A,
isoform 1"
/protein_id="AAH73752.1"
/db_xref="GeneID:1973"
/db_xref="HGNC:HGNC:3282"
/db_xref="MIM:602641"
/translation="MSASQDSRSRDNGPDGMEPEGVIESNWNEIVDSFDDMNLSESLL
RGIYAYGFEKPSAIQQRAILPCIKGYDVIAQAQSGTGKTATFAISILQQIELDLKATQ
ALVLAPTRELAQQIQKVVMALGDYMGASCHACIGGTNVRAEVQKLQMEAPHIIVGTPG
RVFDMLNRRYLSPKYIKMFVLDEADEMLSRGFKDQIYDIFQKLNSNTQVVLLSATMPS
DVLEVTKKFMRDPIRILVKKEELTLEGIRQFYINVEREEWKLDTLCDLYETLTITQAV
IFINTRRKVDWLTEKMHARDFTVSAMHGDMDQKERDVIMREFRSGSSRVLITTDLLAR
GIDVQQVSLVINYDLPTNRENYIHRIGRGGRFGRKGVAINMVTEEDKRTLRDIETFYN
TSIEEMPLNVADLI"
BASE COUNT 378 a 332 c 368 g 316 t
ORIGIN
1 atcatgtctg cgagccagga ttcccgatcc agagacaatg gccccgatgg gatggagccc
61 gaaggcgtca tcgagagtaa ctggaatgag attgttgaca gctttgatga catgaacctc
121 tcggagtccc ttctccgtgg catctacgcc tatggttttg agaagccctc tgccatccag
181 cagcgagcca ttctaccttg tatcaagggt tatgatgtga ttgctcaagc ccaatctggg
241 actgggaaaa cggccacatt tgccatatcg attctgcagc agattgaatt agatctaaaa
301 gccacccagg ccttggtcct agcacccact cgagaattgg ctcagcagat acagaaggtg
361 gtcatggcac taggagacta catgggcgcc tcctgtcacg cctgtatcgg gggcaccaac
421 gtgcgtgctg aggtgcagaa actgcagatg gaagctcccc acatcatcgt gggtacccct
481 ggccgtgtgt ttgatatgct taaccggaga tacctgtccc ccaaatacat caagatgttt
541 gtactggatg aagctgacga aatgttaagc cgtggattca aggaccagat ctatgacata
601 ttccaaaagc tcaacagcaa cacccaggta gttttgctgt cagccacaat gccttctgat
661 gtgcttgagg tgaccaagaa gttcatgagg gaccccattc ggattcttgt caagaaggaa
721 gagttgaccc tggagggtat ccgccagttc tacatcaacg tggaacgaga ggagtggaag
781 ctggacacac tatgtgactt gtatgaaacc ctgaccatca cccaggcagt catcttcatc
841 aacacccgga ggaaggtgga ctggctcacc gagaagatgc atgctcgaga tttcactgta
901 tccgccatgc atggagatat ggaccaaaag gaacgagacg tgattatgag ggagtttcgt
961 tctggctcta gcagagtttt gattaccact gacctgctgg ccagaggcat tgatgtgcag
1021 caggtttctt tagtcatcaa ctatgacctt cccaccaaca gggaaaacta tatccacaga
1081 atcggtcgag gtggacggtt tggccgtaaa ggtgtggcta ttaacatggt gacagaagaa
1141 gacaagagga ctcttcgaga cattgagacc ttctacaaca cctccattga ggaaatgccc
1201 ctcaatgttg ctgacctcat ctgaggggct gtcctgccac ccagccccag ccagggctca
1261 atctctgggg gctgaggagc agcaggaggg gggagggaag ggagccaagg gatggacatc
1321 ttgtcatttt tttctttgaa taaatgtcac tttttgaggc aaaagaagga accgtgaaaa
1381 aaaaaaaaaa aaaa
//