LOCUS BC072430 1409 bp mRNA linear HUM 19-JUN-2006
DEFINITION Homo sapiens cholesterol 25-hydroxylase, mRNA (cDNA clone MGC:88041
IMAGE:5589142), complete cds.
ACCESSION BC072430
VERSION BC072430.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1409)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 1409)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (01-JUN-2004) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: Invitrogen
cDNA Library Preparation: Life Technologies, Inc.
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: National Institutes of Health Intramural
Sequencing Center (NISC),
Gaithersburg, Maryland;
Web site: http://www.nisc.nih.gov/
Contact: nisc_mgc@nhgri.nih.gov
Akhter,N., Ayele,K., Beckstrom-Sternberg,S.M., Benjamin,B.,
Blakesley,R.W., Bouffard,G.G., Breen,K., Brinkley,C., Brooks,S.,
Dietrich,N.L., Granite,S., Guan,X., Gupta,J., Haghighi,P.,
Hansen,N., Ho,S.-L., Karlins,E., Kwong,P., Laric,P., Legaspi,R.,
Maduro,Q.L., Masiello,C., Maskeri,B., Mastrian,S.D.,McCloskey,J.C.,
McDowell,J., Pearson,R., Stantripop,S., Thomas,P.J., Touchman,J.W.,
Tsurgeon,C., Vogt,J.L., Walker,M.A., Wetherby,K.D., Wiggins,L.,
Young,A., Zhang,L.-H. and Green,E.D.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 174 Row: a Column: 10
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 31542304.
FEATURES Location/Qualifiers
source 1..1409
/db_xref="H-InvDB:HIT000264875"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:88041 IMAGE:5589142"
/tissue_type="Ovary, pooled from 3 adults"
/clone_lib="NIH_MGC_125"
/lab_host="DH10B"
/note="Vector: pCMV-SPORT6"
gene 1..1409
/gene="CH25H"
/gene_synonym="C25H"
/db_xref="GeneID:9023"
/db_xref="HGNC:HGNC:1907"
/db_xref="MIM:604551"
CDS 3..821
/gene="CH25H"
/gene_synonym="C25H"
/codon_start=1
/product="cholesterol 25-hydroxylase"
/protein_id="AAH72430.1"
/db_xref="GeneID:9023"
/db_xref="HGNC:HGNC:1907"
/db_xref="MIM:604551"
/translation="MSCHNCSDPQVLCSSGQLFLQPLWDHLRSWEALLQSPFFPVIFS
ITTYVGFCLPFVVLDILCSWVPALRRYKIHPDFSPSAQQLLPCLGQTLYQHVMFVFPV
TLLHWARSPALLPHEAPELLLLLHHILFCLLLFDMEFFVWHLLHHKVPWLYRTFHKVH
HQNSSSFALATQYMSVWELFSLGFFDMMNVTLLGCHPLTTLTFHVVNIWLSVEDHSGY
NFPWSTHRLVPFGWYGGVVHHDLHHSHFNCNFAPYFTHWDKILGTLRTASVPAR"
BASE COUNT 349 a 376 c 294 g 390 t
ORIGIN
1 caatgagctg ccacaactgc tccgaccccc aggtcctttg cagctccggg cagctcttcc
61 tgcagcccct ctgggaccac ctgaggagct gggaggccct cctacagtcg cccttcttcc
121 cggtcatctt ctccatcacc acatacgtgg gcttttgcct gcccttcgtg gtcctggata
181 tcctgtgctc ctgggtgccc gccctgcggc gctacaagat ccaccctgac ttctcgccat
241 ccgcgcagca gctgctacct tgcctggggc agaccctcta ccagcatgtg atgtttgtgt
301 tccccgtgac gctgctgcat tgggcccgca gcccggccct cctgccccac gaagctcccg
361 agctgctcct gctgctgcac cacatcctgt tctgcctgct actcttcgac atggagttct
421 tcgtgtggca cctgctgcac cacaaggtgc cctggctgta ccgcaccttc cacaaggtgc
481 accaccagaa ctcgtcctcg ttcgcactgg caacgcagta tatgagcgtc tgggaactgt
541 tttctttggg cttcttcgac atgatgaacg tcacactgct cgggtgccac ccgctcacca
601 ccctgacctt ccacgtggtc aacatctggc tttccgtgga ggaccactcc ggctacaact
661 tcccttggtc cactcacaga ctggtgccct tcgggtggta cgggggtgtg gtgcaccacg
721 acctgcatca ctctcacttt aactgcaact tcgctccgta ctttacacac tgggacaaaa
781 tactgggaac gctgcggact gcatctgtcc cagcgcggtg atgtggctgc ggtgggtgcc
841 cctaagactc gggactgctg tgcctttcac acttgaatga agagaaacac ctgagctata
901 tattttttta aagcaactaa cttattgctt tatgtttatc tatgaaaacc atagataaaa
961 tctgatgcat ttttgtaatc tgacaaagta atttacatac tgtttgtgta tcaatacaat
1021 tttgtgttct tggtattctt agtctagctc acctcaatag ccttgaatcc tgcatatgaa
1081 ttagacattc atcactggca tatttagaat atctctaaaa ggacttgttt gtagaataag
1141 gaattttcta tgtttcaaag tgttctaaaa cctggctaaa agaatgtatt tttgtggatg
1201 gtgttgactt ctgactctaa aagcaatcaa acatgtttct gctggacagt gaccaagaat
1261 tatagtacct tcttatattt ttttatagaa ctgtatattt attttgaaag aaatgttatt
1321 cgtgctttaa aaaggaaaaa aaaacatgaa tcaaataagt gttgaaaaaa aaaaaaaaaa
1381 aaaaaaaaaa aaaaaaaaaa aaaaaaaaa
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