LOCUS BC072004 3203 bp mRNA linear HUM 25-JUN-2004
DEFINITION Homo sapiens chloride channel 2, mRNA (cDNA clone MGC:88707
IMAGE:6424015), complete cds.
ACCESSION BC072004
VERSION BC072004.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 3203)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 3203)
AUTHORS Strausberg,R.
TITLE Direct Submission
JOURNAL Submitted (01-JUN-2004) National Institutes of Health, Mammalian
Gene Collection (MGC), Cancer Genomics Office, National Cancer
Institute, 31 Center Drive, Room 11A03, Bethesda, MD 20892-2590,
USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: DCTD/DTP/Gazdar
cDNA Library Preparation: Rubin Laboratory
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Sequencing Group at the Stanford Human Genome
Center, Stanford University School of Medicine, Stanford, CA 94305
Web site: http://www-shgc.stanford.edu
Contact: (Dickson, Mark) mcd@paxil.stanford.edu
Dickson, M., Schmutz, J., Grimwood, J., Rodriquez, A., and Myers,
R. M.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 58 Row: m Column: 19
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 5803001.
FEATURES Location/Qualifiers
source 1..3203
/db_xref="H-InvDB:HIT000264809"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:88707 IMAGE:6424015"
/tissue_type="Lung, large cell carcinoma"
/clone_lib="NIH_MGC_18"
/lab_host="DH10B-R"
/note="Vector: pOTB7"
gene 1..3203
/gene="CLCN2"
/gene_synonym="CIC-2"
/gene_synonym="CLC2"
/gene_synonym="ECA3"
/gene_synonym="EGI3"
/gene_synonym="EGMA"
/db_xref="GeneID:1181"
/db_xref="MIM:600570"
CDS 124..2769
/gene="CLCN2"
/gene_synonym="CIC-2"
/gene_synonym="CLC2"
/gene_synonym="ECA3"
/gene_synonym="EGI3"
/gene_synonym="EGMA"
/codon_start=1
/product="CLCN2 protein"
/protein_id="AAH72004.1"
/db_xref="GeneID:1181"
/db_xref="MIM:600570"
/translation="MAAAAAEEGMEPRALQYEQTLMYGRYTQDLGAFAKEEAARIRLG
GPEPWKGPPSSRAAPELLEYGRSRCARCRVCSVRCHKFLVSRVGEDWIFLVLLGLLMA
LVSWVMDYAIAACLQAQQWMSRGLNTSILLQYLAWVTYPVVLITFSAGFTQILAPQAV
GSGIPEMKTILRGVVLKEYLTLKTFIAKVIGLTCALGSGMPLGKEGPFVHIASMCAAL
LSKFLSLFGGIYENESRNTEMLAAACAVGVGCCFAAPIGGVLFSIEVTSTFFAVRNYW
RGFFAATFSAFIFRVLAVWNRDEETITALFKTRFRLDFPFDLQELPAFAVIGIASGFG
GALFVYLNRKIVQVMRKQKTINRFLMRKRLLFPALVTLLISTLTFPPGFGQFMAGQLS
QKETLVTLFDNRTWVRQGLVEELEPPSTSQAWNPPRANVFLTLVIFILMKFWMSALAT
TIPVPCGAFMPVFVIDGIHTDSSTYRIVPGGYAVVGAAALAGAVTHTVSTAVIVFELT
GQIAHILPVMIAVILANAVAQSLQPSLYDSIIRIKKLPYLPELGWGRHQQYRVRVEDI
MVRDVPHVALSCTFRDLRLALHRTKGRMLALVESPESMILLGSIERSQVVALLGAQLS
PARRRQHMQERRATQTSPLSDQEGPPTPEASVCFQVNTEDSAFPAARGETHKPLKPAL
KRGPSVTRNLGESPTGSAESAGIALRSLFCGSPPPEAASEKLESCEKRKLKRVRISLA
SDADLEGEMSPEEILEWEEQQLDEPVNFSDCKIDPAPFQLVERTSLHKTHTIFSLLGV
DHAYVTSIGRLIGIVTLKELRKAIEGSVTAQGVKVRPPLASFRDSATSSSDTETTEVH
ALWGPHSRHGLPREGSPSDSDDKCQ"
BASE COUNT 650 a 966 c 910 g 677 t
ORIGIN
1 ctctgctctc ggcctcccgg gctgcgggga cgggacggct gccggcgcgg actttgcggg
61 ccgggagccg agtccaggac agagccggaa ccgccgaggg aggcgagagg gcagtgcgcg
121 gagatggcgg ccgcggcggc ggaggaaggg atggagccac gggcgctgca gtacgagcag
181 accctgatgt atggccggta cactcaggac cttggggcct ttgccaaaga ggaagctgct
241 cggattcgcc tgggagggcc tgaaccctgg aaaggtcccc cttcctctcg ggctgcccca
301 gagctcttgg aatatggacg gagccgttgc gcccgatgcc gcgtctgttc tgtccgctgc
361 cacaagttcc tagtatccag ggttggtgaa gattggatct tcctggtcct gctggggctt
421 ctcatggcat tggtcagctg ggtcatggac tatgccattg ctgcctgtct gcaagcccag
481 cagtggatgt cccggggctt gaacaccagc atcttgctcc agtacctggc ctgggtcacc
541 taccctgttg tcctcatcac tttctcagcc ggattcacac agatcctggc ccctcaggct
601 gtcggctctg gcatccctga gatgaagacc atcttgcggg gagtggtgct gaaagaatac
661 ctcacactca agacctttat agctaaggtc attgggctga cctgcgccct aggcagcggg
721 atgccgcttg gcaaagaggg cccttttgtg catatcgcaa gcatgtgtgc tgcccttctc
781 agcaagttcc tctccctctt tgggggtatc tatgagaatg aatcccggaa cacagagatg
841 ctggctgccg cctgtgccgt gggggtgggc tgctgcttcg cggcacctat tggaggcgtc
901 ctcttcagca tcgaggtcac ctccaccttc tttgcagtgc ggaactactg gcggggcttc
961 ttcgctgcca ccttcagtgc cttcatcttc cgggtcttgg cagtctggaa ccgggatgaa
1021 gagactatta cagccctctt caaaacccga ttccggctcg acttcccctt tgacctgcag
1081 gagctgccag cctttgctgt cattggtatt gctagtggct tcggtggagc cctctttgtc
1141 tacctgaacc ggaagattgt ccaggtgatg cggaagcaga aaaccatcaa tcgcttcctc
1201 atgaggaaac gcctgctctt cccggctctg gtgaccctgc tcatctccac gctgaccttc
1261 ccccctggct ttggacagtt catggctgga cagctctcac agaaagagac gctggtcacc
1321 ctgtttgaca atcggacgtg ggtccgccag ggcctggtgg aggagctaga accacccagc
1381 acctcacagg cctggaaccc accacgtgcc aacgtcttcc tcaccctggt catcttcatt
1441 ctcatgaagt tctggatgtc tgcactggcc accaccatcc cagttccctg tggggccttc
1501 atgcctgtct ttgtcattga tggaattcat acggacagca gcacctaccg gattgtgcct
1561 gggggctacg ctgtggtcgg ggcagctgcg ctggcaggag cggtgacaca cacagtgtcc
1621 acggctgtga tcgtgttcga gctcacaggc cagattgccc acatcctgcc tgtcatgatc
1681 gccgtcatcc tggccaacgc tgtcgcccag agtctgcagc cctccctcta tgacagcatc
1741 atccgaatca agaaactgcc ctacctgcct gagctcggct ggggccgcca ccagcagtac
1801 cgggtgcgtg tggaggacat catggtgcgg gatgttcccc atgtggccct cagctgcacc
1861 ttccgggacc tgcgtttggc actgcacagg accaagggcc gaatgctggc cctagtggag
1921 tcccctgagt ccatgattct gctgggctcc atcgagcgtt cacaggtggt ggcattgttg
1981 ggggcccagc tgagcccagc ccgccggcgg cagcacatgc aggagcgcag agccacccag
2041 acctctccac tatctgatca ggagggtccc cctacccctg aggcttctgt ctgcttccag
2101 gtgaacacag aagactcagc cttcccagca gcccgggggg agacccacaa gcccctaaag
2161 cctgcactca agagggggcc cagtgtcacc aggaacctcg gagagagtcc cacagggagc
2221 gcagagtcgg caggcatcgc cctccggagc ctcttctgtg gcagtccacc ccctgaggct
2281 gcttcggaga agttggaatc ctgtgagaag cgcaagctga agcgtgtccg aatctccctg
2341 gcaagtgacg cggacctgga aggcgagatg agccctgaag agattctgga gtgggaggag
2401 cagcaactag atgaacctgt caacttcagt gactgcaaaa ttgatcctgc tcccttccag
2461 ctggtggagc ggacctcttt gcacaagact cacactatct tctcactgct gggagtggac
2521 catgcttatg tcaccagtat tggcagactc attggaatcg ttactctaaa ggagctccgg
2581 aaggccatcg agggctctgt cacagcacag ggtgtgaaag tccggccgcc cctcgccagc
2641 ttccgagaca gtgccaccag cagcagtgac acggagacca ctgaggtgca tgcactctgg
2701 gggccccact cccgtcatgg cctcccccgg gagggcagcc cttccgacag cgacgacaaa
2761 tgccaatgag cccctcgtgg gtggcctagg atggtgctag ccatgcccgt cagcccagaa
2821 tgtgcatctt tcattccttc tgccttcgga aggcaggagg cagctacagc tggaggctgc
2881 accccagccc cctccagacc tggggtgcca gcttctccca gttcatccta cctggaatct
2941 gacccactac ccacctgcaa caagtcttcc agaggcagga agataggccc tgccctggca
3001 ggatgggttg gggtcacttg acccctgctc cccctttgag gggaaagggg tggaactaag
3061 atgggtttat aactggaacc tccaatgacc agatgtatat agagatttac aaagattttt
3121 atattaattt aataaaacaa attcttaaat agaacaaaat aaacacctaa tgagccactt
3181 atatataaaa aaaaaaaaaa aaa
//