LOCUS       BC071996                1133 bp    mRNA    linear   HUM 06-JUL-2005
DEFINITION  Homo sapiens dihydrofolate reductase, mRNA (cDNA clone MGC:88697
            IMAGE:4645596), complete cds.
ACCESSION   BC071996
VERSION     BC071996.1
KEYWORDS    MGC.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1133)
  AUTHORS   Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
            Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
            Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
            Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
            Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
            Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
            Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
            Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
            Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
            McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
            Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
            Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
            Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
            Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
            Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
            Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
            Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
            Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
  CONSRTM   Mammalian Gene Collection Program Team
  TITLE     Generation and initial analysis of more than 15,000 full-length
            human and mouse cDNA sequences
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
   PUBMED   12477932
REFERENCE   2  (bases 1 to 1133)
  CONSRTM   NIH MGC Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-JUN-2004) National Institutes of Health, Mammalian
            Gene Collection (MGC), Bethesda, MD 20892-2590, USA
  REMARK    NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT     Contact: MGC help desk
            Email: cgapbs-r@mail.nih.gov
            Tissue Procurement: ATCC
            cDNA Library Preparation: Rubin Laboratory
            cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
            DNA Sequencing by: Sequencing Group at the Stanford Human Genome
            Center, Stanford University School of Medicine, Stanford, CA  94305
            Web site:       http://www-shgc.stanford.edu
            Contact:  (Dickson, Mark) mcd@paxil.stanford.edu
            Dickson, M., Schmutz, J., Grimwood, J., Rodriquez, A., and Myers,
            R. M.
            
            Clone distribution: MGC clone distribution information can be found
            through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
            Series: IRAL Plate: 58 Row: d Column: 17.
FEATURES             Location/Qualifiers
     source          1..1133
                     /db_xref="H-InvDB:HIT000264802"
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /clone="MGC:88697 IMAGE:4645596"
                     /tissue_type="Eye, retinoblastoma"
                     /clone_lib="NIH_MGC_16"
                     /lab_host="DH10B-R"
                     /note="Vector: pOTB7"
     gene            1..1133
                     /gene="DHFR"
                     /db_xref="GeneID:1719"
                     /db_xref="MIM:126060"
     CDS             455..1018
                     /gene="DHFR"
                     /codon_start=1
                     /product="DHFR protein"
                     /protein_id="AAH71996.1"
                     /db_xref="GeneID:1719"
                     /db_xref="MIM:126060"
                     /translation="MVGSLNCIVAVSQNMGIGKNGDLPWPPLRNEFRYFQRMTTTSSV
                     EGKQNLVIMGKKTWFSIPEKNRPLKGRINLVLSRELKEPPQGAHFLSRSLDDALKLTE
                     QPELANKVDMVWIVGGSSVYKEAMNHPGHLKLFVTRIMQDFESDTFFPEIDLEKYKLL
                     PEYPGVLSDVQEEKGIKYKFEVYEKND"
BASE COUNT          295 a          263 c          329 g          246 t
ORIGIN      
        1 aacctactac gtgcggcggc agctggggcg ggaaggcggg cgctgggggc gctgcggccg
       61 ctgcagcgcc agggtccacc tggtcggctg cacctgtgga ggaggaggtg gatttcaggc
      121 ttcccgtaga ctggaagaat cggctcaaaa ccgcttgcct cgcaggggct gagctggagg
      181 cagcgaggcc gcccgacgca ggcttccggc gagacatggc agggcaagga tggcagcccg
      241 gcggcagggc ccggcgagga gcgcgaaccc gcggccgcag ttcccaggcg tctgcgggcg
      301 cgagcacgcc gcgaccctgc gtgcgccggg gcgggggggc ggggcctcgc ctgcacaaat
      361 agggacgagg gggcggggcg gccacaattt cgcgccaaac ttgaccgcgc gttctgctgt
      421 aacgagcggg ctcggaggtc ctcccgctgc tgtcatggtt ggttcgctaa actgcatcgt
      481 cgctgtgtcc cagaacatgg gcatcggcaa gaacggggac ctgccctggc caccgctcag
      541 gaatgaattc agatatttcc agagaatgac cacaacctct tcagtagaag gtaaacagaa
      601 tctggtgatt atgggtaaga agacctggtt ctccattcct gagaagaatc gacctttaaa
      661 gggtagaatt aatttagttc tcagcagaga actcaaggaa cctccacaag gagctcattt
      721 tctttccaga agtctagatg atgccttaaa acttactgaa caaccagaat tagcaaataa
      781 agtagacatg gtctggatag ttggtggcag ttctgtttat aaggaagcca tgaatcaccc
      841 aggccatctt aaactatttg tgacaaggat catgcaagac tttgaaagtg acacgttttt
      901 tccagaaatt gatttggaga aatataaact tctgccagaa tacccaggtg ttctctctga
      961 tgtccaggag gagaaaggca ttaagtacaa atttgaagta tatgagaaga atgattaata
     1021 tgaaggtgtt ttctagttta agttgttccc cctccctctg aaaaaagtat gtatttttac
     1081 attagaaaag gttttttgtt gactttagat ctataaaaaa aaaaaaaaaa aaa
//