LOCUS BC071758 1472 bp mRNA linear HUM 25-JUN-2004
DEFINITION Homo sapiens T cell receptor alpha locus, mRNA (cDNA clone
MGC:88333 IMAGE:30416838), complete cds.
ACCESSION BC071758
VERSION BC071758.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1472)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 1472)
AUTHORS Strausberg,R.
TITLE Direct Submission
JOURNAL Submitted (01-JUN-2004) National Institutes of Health, Mammalian
Gene Collection (MGC), Cancer Genomics Office, National Cancer
Institute, 31 Center Drive, Room 11A03, Bethesda, MD 20892-2590,
USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: Narayan Bhat
cDNA Library Preparation: CLONTECH Laboratories, Inc.
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Sequencing Group at the Stanford Human Genome
Center, Stanford University School of Medicine, Stanford, CA 94305
Web site: http://www-shgc.stanford.edu
Contact: (Dickson, Mark) mcd@paxil.stanford.edu
Dickson, M., Schmutz, J., Grimwood, J., Rodriquez, A., and Myers,
R. M.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 57 Row: p Column: 11
This clone was selected for full length sequencing because it
passed the following selection criteria: Hexamer frequency ORF
analysis, GenomeScan gene prediction, Similarity but not identity
to protein.
FEATURES Location/Qualifiers
source 1..1472
/db_xref="H-InvDB:HIT000264578"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:88333 IMAGE:30416838"
/tissue_type="Blood, peripheral blood mononuclear cells
(PBMC) cytoplasmic poly A RNA is pooled sample from 3 and
6 hr after stimulation with PMA and Ionomycin. These
reagents are used to activate T- cells that results in
lymphokine and cytokine production."
/clone_lib="NIH_MGC_191"
/lab_host="DH10B"
/note="Vector: pDNR-LIB"
gene 1..1472
/gene="TRA@"
/gene_synonym="TCRA"
/gene_synonym="TRA"
/db_xref="GeneID:6955"
/db_xref="MIM:186880"
CDS 55..891
/gene="TRA@"
/gene_synonym="TCRA"
/gene_synonym="TRA"
/codon_start=1
/product="TRA@ protein"
/protein_id="AAH71758.1"
/db_xref="GeneID:6955"
/db_xref="MIM:186880"
/translation="MAMLLGASVLILWLQPDWVNSQQKNDDQQVKQNSPSLSVQEGRI
SILNCDYTNSMFDYFLWYKKYPAEGPTFLISISSIKDKNEDGRFTVFLNKSAKHLSLH
IVPSQPGDSAVYFCAVRYNNNDMRFGAGTRLTVKPNIQNPDPAVYQLRDSKSSDKSVC
LFTDFDSQTNVSQSKDSDVYITDKTVLDMRSMDFKSNSAVAWSNKSDFACANAFNNSI
IPEDTFFPSPESSCDVKLVEKSFETDTNLNFQNLSVIGFRILLLKVAGFNLLMTLRLW
SS"
BASE COUNT 405 a 372 c 318 g 377 t
ORIGIN
1 agatttgcag ctttctaggc aggagacaag acaatctgca tcttcacagg agggatggcc
61 atgctcctgg gggcatcagt gctgattctg tggcttcagc cagactgggt aaacagtcaa
121 cagaagaatg atgaccagca agttaagcaa aattcaccat ccctgagcgt ccaggaagga
181 agaatttcta ttctgaactg tgactatact aacagcatgt ttgattattt cctatggtac
241 aaaaaatacc ctgctgaagg tcctacattc ctgatatcta taagttccat taaggataaa
301 aatgaagatg gaagattcac tgtcttctta aacaaaagtg ccaagcacct ctctctgcac
361 attgtgccct cccagcctgg agactctgca gtgtacttct gtgcggtccg atacaataac
421 aatgacatgc gctttggagc agggaccaga ctgacagtaa aaccaaatat ccagaaccct
481 gaccctgccg tgtaccagct gagagactct aaatccagtg acaagtctgt ctgcctattc
541 accgattttg attctcaaac aaatgtgtca caaagtaagg attctgatgt gtatatcaca
601 gacaaaactg tgctagacat gaggtctatg gacttcaaga gcaacagtgc tgtggcctgg
661 agcaacaaat ctgactttgc atgtgcaaac gccttcaaca acagcattat tccagaagac
721 accttcttcc ccagcccaga aagttcctgt gatgtcaagc tggtcgagaa aagctttgaa
781 acagatacga acctaaactt tcaaaacctg tcagtgattg ggttccgaat cctcctcctg
841 aaagtggccg ggtttaatct gctcatgacg ctgcggctgt ggtccagctg agatctgcaa
901 gattgtaaga cagcctgtgc tccctcgctc cttcctctgc attgcccctc ttctccctct
961 ccaaacagag ggaactctcc tacccccaag gaggtgaaag ctgctaccac ctctgtgccc
1021 ccccggcaat gccaccaact ggatcctaca cgaatttatg attaagattg ctgaagagct
1081 gccaaacact gctgccaccc cctctgttcc cttattgctg cttgtcactg cctgacattc
1141 acggcagagg caaggctgct gcagcctccc ctggctgtgc acattccctc ctgctcccca
1201 gagactgcct ccgccatccc acagatgatg gatcttcagt gggttctctt gggctctagg
1261 tcccggagaa tgttgtgagg ggtttatttt tttttaatag tgttcataaa gaaagacata
1321 gtattcttct tctcaagacg tggggggaaa ttatctcatt atcgaggccc tgctatgctg
1381 tgtgtctggg cgtgttgtat gtcctgctgc cgatgccttc attaaaatga tttggaagag
1441 cgaaaaaaaa aaaaaaaaaa aaaaaaaaaa aa
//