LOCUS BC071637 2741 bp mRNA linear HUM 28-JUL-2005
DEFINITION Homo sapiens BRF1 homolog, subunit of RNA polymerase III
transcription initiation factor IIIB (S. cerevisiae), mRNA (cDNA
clone IMAGE:4177747), partial cds.
ACCESSION BC071637
VERSION BC071637.1
KEYWORDS .
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2741)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 2741)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (01-JUN-2004) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: David N. Louis, M.D.
cDNA Library Preparation: Life Technologies, Inc.
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Sequencing Group at the Stanford Human Genome
Center, Stanford University School of Medicine, Stanford, CA 94305
Web site: http://www-shgc.stanford.edu
Contact: (Dickson, Mark) mcd@paxil.stanford.edu
Dickson, M., Schmutz, J., Grimwood, J., Rodriquez, A., and Myers,
R. M.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 166 Row: a Column: 12
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 22035557.
FEATURES Location/Qualifiers
source 1..2741
/db_xref="H-InvDB:HIT000264465"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="IMAGE:4177747"
/tissue_type="Brain, anaplastic oligodendroglioma with
1p/19q loss"
/clone_lib="NCI_CGAP_Brn67"
/lab_host="DH10B"
/note="Vector: pCMV-SPORT6"
gene <1..2741
/gene="BRF1"
/gene_synonym="BRF"
/gene_synonym="hBRF"
/gene_synonym="TAFIII90"
/gene_synonym="TF3B90"
/gene_synonym="TFIIIB90"
/db_xref="GeneID:2972"
/db_xref="MIM:604902"
CDS <1..1461
/gene="BRF1"
/gene_synonym="BRF"
/gene_synonym="hBRF"
/gene_synonym="TAFIII90"
/gene_synonym="TF3B90"
/gene_synonym="TFIIIB90"
/codon_start=1
/product="BRF1 protein"
/protein_id="AAH71637.1"
/db_xref="GeneID:2972"
/db_xref="MIM:604902"
/translation="HLLEFGEKNHEVSMTALRLLQRMKRDWMHTGRRPSGLCGAALLV
AARMHDFRRTVKEVISVVKVCESTLRKRLTEFEDTPTSQLTIDEFMKIDLEEECDPPS
YTAGQRKLRMKQLEQVLSKKLEEVEGEISSYQDAIEIELENSRPKAKGGLASLAKDGS
TEDTASSLCGEEDTEDEELEAAASHLNKDLYRELLGGAPGSSEAAGSPEWGGRPPALG
SLLDPLPTAASLGISDSIRECISSQSSDPKDASGDGELDLSGIDDLEIDRYILNESEA
RVKAELWMRENAEYLREQREKEARIAKEKELGIYKEHKPKKSCKRREPIQASTAREAI
EKMLEQKKISSKINYSVLRGLSSAGGGSPHREDAQPEHSASARKLSRRRTPASRSGAD
PVTSVGKRLRPLVSTQPAKKVATGEALLPSSPTLGAEPARPQAVLVESGPVSYHADEE
ADEEEPDEEDGEPCVSALQMMGSNDYGCDGDEDDGY"
BASE COUNT 598 a 804 c 898 g 441 t
ORIGIN
1 cacctgctgg aattcgggga gaagaaccac gaggtgtcca tgactgccct gaggctccta
61 cagaggatga agcgggactg gatgcacaca ggccggcgcc cctcgggcct ctgcggagca
121 gcgctcctgg ttgcagccag aatgcatgac ttcaggagga ctgtgaagga ggtcatcagt
181 gtggtcaaag tgtgtgagtc cacgctgcgg aagaggctca cggaatttga agacaccccc
241 accagtcagt tgaccattga tgagttcatg aagatcgacc tggaggagga gtgcgacccc
301 ccctcgtaca cagctgggca gaggaagctg cggatgaagc agcttgaaca agtcctgtca
361 aaaaaactgg aggaggttga aggtgaaata tccagttacc aggatgcaat tgagattgaa
421 ctagaaaaca gccggccaaa ggccaagggg ggcctggcca gcctggcaaa agatggctcc
481 accgaggaca ccgcgtccag cttgtgtggc gaggaggaca cagaggacga ggagctggaa
541 gccgcggcca gccacctgaa caaagactta taccgggagc tccttggtgg tgcccccggc
601 agctcggaag cagcaggaag ccccgagtgg ggcggcagac ctccggccct ggggtccctg
661 ctggaccccc tccccactgc agccagcctg ggcatctcag actccatccg cgaatgcatc
721 tcctctcaga gcagcgaccc caaagatgct tcaggagacg gtgagctgga cctcagtggc
781 attgatgacc tggagattga caggtacatc ctgaatgagt cggaagcccg cgtgaaggcc
841 gagctgtgga tgagggagaa cgccgagtac ctgcgggaac agagggaaaa agaagcaaga
901 atagcgaaag agaaggagct cggcatctac aaggaacaca agcccaagaa gtcttgcaag
961 cgacgggagc caattcaggc cagtaccgcc agggaggcca tcgagaagat gctggagcag
1021 aagaagatct ccagcaagat caattatagc gtgctccggg gcctcagcag cgccggcggg
1081 ggcagtccgc acagggagga tgcacagccc gagcatagcg ccagtgccag gaagctgtca
1141 cgaaggagga cgccggccag cagaagtggg gctgaccctg tgaccagtgt ggggaaaagg
1201 ttgaggcctc tggtgtctac gcagccagca aagaaggtgg ccacgggaga ggctttgctc
1261 ccaagctctc ccaccctcgg agctgagcct gccaggcccc aggcggtgct ggtggagagc
1321 gggcccgtgt cataccacgc cgacgaggag gctgacgagg aggagcctga cgaggaggac
1381 ggggagccct gcgtcagtgc cctgcagatg atgggcagca acgactatgg ctgtgatggc
1441 gatgaggacg acggctactg aagtgtggcc tccaggcagg tgatgtcctg gcagggggcc
1501 tcgcgggtct cctcagcatc agacgggctt ccaggaccgc agcaggcagg ccccagcgcc
1561 gagactcctg gtgacaggtg gcacctgtcc cacagccctc gtcccatgtg gaacttacca
1621 ttgggattgt gtttctattc agcaagggaa accggaccaa gcgtctgcat gtgtgtgatc
1681 agatgtgggc cgggtgtgtg cagggctggg tcccgctgcc tgccgtcgac tcatccaagg
1741 accctccaag gctggcagtg tggtgttgct actattaagg aaacaggctt ggggcagccc
1801 cactgctggt ccaagtgtgt ggagggctga gtgtgctggc cctgtgactc aggaccagct
1861 ctggagtctc cagcccaccc tccgcaccgt cccctcctga gcagcactcg gcgccagcag
1921 cctctgccag agtggaagcc agagccctgc aggtgtccgg cgcagccgtg ggagctgagg
1981 atctggcact tgagaggcag cagctccttg aaggtcctct gcctccagct gtggccctgc
2041 atccagatac ctgcctcgtc cgaggcagac acccccaccc ctgcctcctc cagacccccc
2101 tccccgctgc ctgcaccgcc tggagcagca tgggggtcag acccctgctc cagggccact
2161 tgagttgtgg gcccaggagc cctgcggctg ccggcaggtg aactgagtgc ccgacagctg
2221 agaccggcgc ccacccgtcc tgagcatagc tctgtaggca gtgcgggcat agcctgcata
2281 gtgtcctggc gctgggagtt ccccgtggac agagccagag ggcagtggcg ctccctgtca
2341 gagctggatc aggcccccca tcgaggaggg agggcagacg gaggcccgag agcctcccca
2401 ggcctcttcg tgggaaggcc ccagtaccac tcgtaggagg tctcagctct ggcatggctg
2461 ccccggatgt ggccgagggg gcttcaccct gtgtccttag gagggggtgg ccttgaggca
2521 gagccgtgcc tcactgaccc ccaggggcct catcctcccc atggaatggg ctgtatgtcc
2581 tgccccaact tggcccgcag caggccagac ccccctaccc ccgcccagag ctcagtagcc
2641 agcctgggtc ctgccagggc ttctcgaggg cttgggggaa gaatagattt agtaaagcag
2701 gaagatctgt tgttacttaa cagaaaaaaa aaaaaaaaaa a
//