LOCUS BC070345 1601 bp mRNA linear HUM 25-JUN-2004
DEFINITION Homo sapiens T cell receptor alpha locus, mRNA (cDNA clone
IMAGE:30418790), partial cds.
ACCESSION BC070345
VERSION BC070345.1
KEYWORDS .
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1601)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 1601)
AUTHORS Strausberg,R.
TITLE Direct Submission
JOURNAL Submitted (10-MAY-2004) National Institutes of Health, Mammalian
Gene Collection (MGC), Cancer Genomics Office, National Cancer
Institute, 31 Center Drive, Room 11A03, Bethesda, MD 20892-2590,
USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: Narayan Bhat
cDNA Library Preparation: CLONTECH Laboratories, Inc.
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Sequencing Group at the Stanford Human Genome
Center, Stanford University School of Medicine, Stanford, CA 94305
Web site: http://www-shgc.stanford.edu
Contact: (Dickson, Mark) mcd@paxil.stanford.edu
Dickson, M., Schmutz, J., Grimwood, J., Rodriquez, A., and Myers,
R. M.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 57 Row: p Column: 3
This clone was selected for full length sequencing because it
passed the following selection criteria: Hexamer frequency ORF
analysis, Similarity but not identity to protein.
FEATURES Location/Qualifiers
source 1..1601
/db_xref="H-InvDB:HIT000264300"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="IMAGE:30418790"
/tissue_type="Blood, peripheral blood mononuclear cells
(PBMC) cytoplasmic poly A RNA is pooled sample from 3 and
6 hr after stimulation with PMA and Ionomycin. These
reagents are used to activate T- cells that results in
lymphokine and cytokine production."
/clone_lib="NIH_MGC_191"
/lab_host="DH10B"
/note="Vector: pDNR-LIB"
BASE COUNT 496 a 379 c 341 g 385 t
ORIGIN
1 gtgagaaacc cctatggctg ccagaggaga gaagagacaa cctgatgata gaagtaactc
61 ttataactgg aggttgcagg tcaatgactg atcttaattg ggaagaacaa ggatgacatc
121 cattcgagct gtatttatat tcctgtggct gcagctggac ttggtgaatg gagagaatgt
181 ggagcagcat ccttcaaccc tgagtgtcca ggagggagac agcgctgtta tcaagtgtac
241 ttattcagac agtgcctcaa actacttccc ttggtataag caagaacttg gaaaaagacc
301 tcagcttatt atagacattc gttcaaatgt gggcgaaaag aaagaccaac gaattgctgt
361 tacattgaac aagacagcca aacatttctc cctgcacatc acagagaccc aacctgaaga
421 ctcggctgtc tacttctgtg cagcaagtga atttcggcgg tagccagaaa acctcctacg
481 acaaggtgat atttgggcca gggacaagct tatcagtcat tccaaatatc cagaaccctg
541 accctgccgt gtaccagctg agagactcta aatccagtga caagtctgtc tgcctattca
601 ccgattttga ttctcaaaca aatgtgtcac aaagtaagga ttctgatgtg tatatcacag
661 acaaaactgt gctagacatg aggtctatgg acttcaagag caacagtgct gtggcctgga
721 gcaacaaatc tgactttgca tgtgcaaacg ccttcaacaa cagcattatt ccagaagaca
781 ccttcttccc cagcccagaa agttcctgtg atgtcaagct ggtcgagaaa agctttgaaa
841 cagatacgaa cctaaacttt caaaacctgt cagtgattgg gttccgaatc ctcctcctga
901 aagtggccgg gtttaatctg ctcatgacgc tgcggctgtg gtccagctga gatctgcaag
961 attgtaagac agcctgtgct ccctcgctcc ttcctctgca ttgcccctct tctccctctc
1021 caaacagagg gaactctcct acccccaagg aggtgaaagc tgctaccacc tctgtgcccc
1081 cccggcaatg ccaccaactg gatcctacac gaatttatga ttaagattgc tgaagagctg
1141 ccaaacactg ctgccacccc ctctgttccc ttattgctgc ttgtcactgc ctgacattca
1201 cggcagaggc aaggctgctg cagcctcccc tggctgtgca cattccctcc tgctccccag
1261 agactgcctc cgccatccca cagatgatgg atcttcagtg ggttctcttg ggctctaggt
1321 cccggagaat gttgtgaggg gtttattttt ttttaatagt gttcataaag aaagacatag
1381 tattcttctt ctcaagacgt ggggggaaat tatctcatta tcgaggccct gctatgctgt
1441 gtgtctgggc gtgttgtatg tcctgctgcc gatgccttca ttaaaatgat ttggaagagc
1501 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
1561 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa a
//