LOCUS BC070344 1430 bp mRNA linear HUM 11-MAY-2004
DEFINITION Homo sapiens T cell receptor alpha locus, mRNA (cDNA clone
MGC:88349 IMAGE:30413659), complete cds.
ACCESSION BC070344
VERSION BC070344.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1430)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 1430)
AUTHORS Strausberg,R.
TITLE Direct Submission
JOURNAL Submitted (10-MAY-2004) National Institutes of Health, Mammalian
Gene Collection (MGC), Cancer Genomics Office, National Cancer
Institute, 31 Center Drive, Room 11A03, Bethesda, MD 20892-2590,
USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: Narayan Bhat
cDNA Library Preparation: CLONTECH Laboratories, Inc.
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Sequencing Group at the Stanford Human Genome
Center, Stanford University School of Medicine, Stanford, CA 94305
Web site: http://www-shgc.stanford.edu
Contact: (Dickson, Mark) mcd@paxil.stanford.edu
Dickson, M., Schmutz, J., Grimwood, J., Rodriquez, A., and Myers,
R. M.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 57 Row: p Column: 23
This clone was selected for full length sequencing because it
passed the following selection criteria: Hexamer frequency ORF
analysis, Similarity but not identity to protein.
FEATURES Location/Qualifiers
source 1..1430
/db_xref="H-InvDB:HIT000264299"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:88349 IMAGE:30413659"
/tissue_type="Blood, peripheral blood mononuclear cells
(PBMC) cytoplasmic poly A RNA is pooled sample from 3 and
6 hr after stimulation with PMA and Ionomycin. These
reagents are used to activate T- cells that results in
lymphokine and cytokine production."
/clone_lib="NIH_MGC_191"
/lab_host="DH10B"
/note="Vector: pDNR-LIB"
gene 1..1430
/gene="TRA@"
/gene_synonym="TCRA"
/gene_synonym="TRA"
/db_xref="GeneID:6955"
/db_xref="MIM:186880"
CDS 99..857
/gene="TRA@"
/gene_synonym="TCRA"
/gene_synonym="TRA"
/codon_start=1
/product="TRA@ protein"
/protein_id="AAH70344.1"
/db_xref="GeneID:6955"
/db_xref="MIM:186880"
/translation="MEGPVTLSEEAFLTINCTYTATGYPSLFWYVQYPGEGLQLLLKA
TKADDKGSNKGFEATYRKETTSFHLEKGSVQVSDSAVYFCALKDADAGGTSYGKLTFG
QGTILTVHPNIQNPDPAVYQLRDSKSSDKSVCLFTDFDSQTNVSQSKDSDVYITDKTV
LDMRSMDFKSNSAVAWSNKSDFACANAFNNSIIPEDTFFPSPESSCDVKLVEKSFETD
TNLNFQNLSVIGFRILLLKVAGFNLLMTLRLWSS"
BASE COUNT 381 a 370 c 311 g 368 t
ORIGIN
1 gaccattatt tgggtaacac actaaagatg aactattctc caggcttagt atctctgata
61 ctcttactgc ttgaacccgt ggagattcag ttacccagat ggaagggcca gtgactctct
121 cagaagaggc cttcctgact ataaactgca cgtacacagc cacaggatac ccttcccttt
181 tctggtatgt ccaatatcct ggagaaggtc tacagctcct cctgaaagcc acgaaggctg
241 atgacaaggg aagcaacaaa ggttttgaag ccacataccg taaagaaacc acttctttcc
301 acttggagaa aggctcagtt caagtgtcag actcagcggt gtacttctgt gctctgaaag
361 atgcggatgc tggtggtact agctatggaa agctgacatt tggacaaggg accatcttga
421 ctgtccatcc aaatatccag aaccctgacc ctgccgtgta ccagctgaga gactctaaat
481 ccagtgacaa gtctgtctgc ctattcaccg attttgattc tcaaacaaat gtgtcacaaa
541 gtaaggattc tgatgtgtat atcacagaca aaactgtgct agacatgagg tctatggact
601 tcaagagcaa cagtgctgtg gcctggagca acaaatctga ctttgcatgt gcaaacgcct
661 tcaacaacag cattattcca gaagacacct tcttccccag cccagaaagt tcctgtgatg
721 tcaagctggt cgagaaaagc tttgaaacag atacgaacct aaactttcaa aacctgtcag
781 tgattgggtt ccgaatcctc ctcctgaaag tggccgggtt taatctgctc atgacgctgc
841 ggctgtggtc cagctgagat ctgcaagatt gtaagacagc ctgtgctccc tcgctccttc
901 ctctgcattg cccctcttct ccctctccaa acagagggaa ctctcctacc cccaaggagg
961 tgaaagctgc taccacctct gtgccccccc ggcaatgcca ccaactggat cctacacgaa
1021 tttatgatta agattgctga agagctgcca aacactgctg ccacccccac tgttccctta
1081 ttgctgcttg tcactgcctg acattcacgg cagaggcaag gctgctgcag cctcccctgg
1141 ctgtgcacat tccctcctgc tccccaggga ctgcctccgc catcccacag atgatggatc
1201 ttcagtgggt tctcttgggc tctaggtccc ggagaatgtt gtgaggggtt tatttttttt
1261 taatagtgtt cataaagaaa gacatagtat tcttcttctc aagacgtggg gggaaattat
1321 ctcattatcg aggccctgct atgctgtgtg tctgggcgtg ttgtatgtcc tgctgccgat
1381 gccttcatta aaatgatttg aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
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