LOCUS BC070329 1587 bp mRNA linear HUM 11-MAY-2004
DEFINITION Homo sapiens T cell receptor alpha locus, mRNA (cDNA clone
MGC:88332 IMAGE:30411883), complete cds.
ACCESSION BC070329
VERSION BC070329.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1587)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 1587)
AUTHORS Strausberg,R.
TITLE Direct Submission
JOURNAL Submitted (10-MAY-2004) National Institutes of Health, Mammalian
Gene Collection (MGC), Cancer Genomics Office, National Cancer
Institute, 31 Center Drive, Room 11A03, Bethesda, MD 20892-2590,
USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: Narayan Bhat
cDNA Library Preparation: CLONTECH Laboratories, Inc.
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Sequencing Group at the Stanford Human Genome
Center, Stanford University School of Medicine, Stanford, CA 94305
Web site: http://www-shgc.stanford.edu
Contact: (Dickson, Mark) mcd@paxil.stanford.edu
Dickson, M., Schmutz, J., Grimwood, J., Rodriquez, A., and Myers,
R. M.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 57 Row: o Column: 23
This clone was selected for full length sequencing because it
passed the following selection criteria: Hexamer frequency ORF
analysis, GenomeScan gene prediction, Similarity but not identity
to protein.
FEATURES Location/Qualifiers
source 1..1587
/db_xref="H-InvDB:HIT000264284"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:88332 IMAGE:30411883"
/tissue_type="Blood, peripheral blood mononuclear cells
(PBMC) cytoplasmic poly A RNA is pooled sample from 3 and
6 hr after stimulation with PMA and Ionomycin. These
reagents are used to activate T- cells that results in
lymphokine and cytokine production."
/clone_lib="NIH_MGC_191"
/lab_host="DH10B"
/note="Vector: pDNR-LIB"
gene 1..1587
/gene="TRA@"
/gene_synonym="TCRA"
/gene_synonym="TRA"
/db_xref="GeneID:6955"
/db_xref="MIM:186880"
CDS 288..1007
/gene="TRA@"
/gene_synonym="TCRA"
/gene_synonym="TRA"
/codon_start=1
/product="TRA@ protein"
/protein_id="AAH70329.1"
/db_xref="GeneID:6955"
/db_xref="MIM:186880"
/translation="MDCAEGRAANLPCNHSTISGNEYVYWYRQIHSQGPQYIIHGLKN
NETNEMASLIITEDRKSSTLILPHATLRDTAVYYCIPGSYIPTFGRGTSLIVHPYIQN
PDPAVYQLRDSKSSDKSVCLFTDFDSQTNVSQSKDSDVYITDKTVLDMRSMDFKSNSA
VAWSNKSDFACANAFNNSIIPEDTFFPSPESSCDVKLVEKSFETDTNLNFQNLSVIGF
RILLLKVAGFNLLMTLRLWSS"
BASE COUNT 437 a 411 c 334 g 405 t
ORIGIN
1 aggaacatga ctaaacctgg ggaggaagac atagaaaagc aactgctcta tcattggctg
61 acaagatcct gacaacaaca caatgagagc tgcactcctt gcaagtcttt actagtagct
121 gttgcttctg ttctctggaa attctttaaa cctagaatca gacacaaaaa ctgaactctg
181 ggtccacaat cctcatttgt ccttgaagta tgaggctggt ggcaagagta actgtgtttc
241 tgacctttgg aactataatt gatgctaaga ccacccagcc cccctccatg gattgcgctg
301 aaggaagagc tgcaaacctg ccttgtaatc actctaccat cagtggaaat gagtatgtgt
361 attggtatcg acagattcac tcccaggggc cacagtatat cattcatggt ctaaaaaaca
421 atgaaaccaa tgaaatggcc tctctgatca tcacagaaga cagaaagtcc agcaccttga
481 tcctgcccca cgctacgctg agagacactg ctgtgtacta ttgcatcccc ggaagctaca
541 tacctacatt tggaagagga accagcctta ttgttcatcc gtatatccag aaccctgacc
601 ctgccgtgta ccagctgaga gactctaaat ccagtgacaa gtctgtctgc ctattcaccg
661 attttgattc tcaaacaaat gtgtcacaaa gtaaggattc tgatgtgtat atcacagaca
721 aaactgtgct agacatgagg tctatggact tcaagagcaa cagtgctgtg gcctggagca
781 acaaatctga ctttgcatgt gcaaacgcct tcaacaacag cattattcca gaagacacct
841 tcttccccag cccagaaagt tcctgtgatg tcaagctggt cgagaaaagc tttgaaacag
901 atacgaacct aaactttcaa aacctgtcag tgattgggtt ccgaatcctc ctcctgaaag
961 tggccgggtt taatctgctc atgacgctgc ggctgtggtc cagctgagat ctgcaagatt
1021 gtaagacagc ctgtgctccc tcgctccttc ctctgcattg cccctcttct ccctctccaa
1081 acagagggaa ctctcccacc cccaaggagg tgaaagctgc taccacctct gtgccccccc
1141 ggcaatgcca ccaactggat cctacccgaa tttatgatta agattgctga agagctgcca
1201 aacactgctg ccaccccctc tgttccctta ttgctgcttg tcactgcctg acattcacgg
1261 cagaggcaag gctgctgcag cctcccctgg ctgtgcacat tccctcctgc tccccagaga
1321 ctgcctccgc catcccacag atgatggatc ttcagtgggt tctcttgggc tctaggtccc
1381 ggagaatgtt gtgaggggtt tatttttttt taatagtgtt cataaagaaa gacatagtat
1441 tcttcttctc aagacgtggg gggaaattat ctcattatcg aggccctgct atgctgtgtg
1501 tctgggcgtg ttgtatgtcc tgctgccgat gccttcatta aaatgatttg gaagaacaaa
1561 aaaaaaaaaa aaaaaaaaaa aaaaaaa
//