LOCUS BC070162 914 bp mRNA linear HUM 08-DEC-2006
DEFINITION Homo sapiens cytotoxic T-lymphocyte-associated protein 4, mRNA
(cDNA clone MGC:88142 IMAGE:30417685), complete cds.
ACCESSION BC070162
VERSION BC070162.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 914)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 914)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (10-MAY-2004) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: Narayan Bhat
cDNA Library Preparation: CLONTECH Laboratories, Inc.
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Sequencing Group at the Stanford Human Genome
Center, Stanford University School of Medicine, Stanford, CA 94305
Web site: http://www-shgc.stanford.edu
Contact: (Dickson, Mark) mcd@paxil.stanford.edu
Dickson, M., Schmutz, J., Grimwood, J., Rodriquez, A., and Myers,
R. M.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 57 Row: p Column: 9
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 21361211.
FEATURES Location/Qualifiers
source 1..914
/db_xref="H-InvDB:HIT000264120"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:88142 IMAGE:30417685"
/tissue_type="Blood, peripheral blood mononuclear cells
(PBMC) cytoplasmic poly A RNA is pooled sample from 3 and
6 hr after stimulation with PMA and Ionomycin. These
reagents are used to activate T- cells that results in
lymphokine and cytokine production."
/clone_lib="NIH_MGC_191"
/lab_host="DH10B"
/note="Vector: pDNR-LIB"
gene 1..914
/gene="CTLA4"
/gene_synonym="CD152"
/gene_synonym="CELIAC3"
/gene_synonym="CTLA-4"
/gene_synonym="GSE"
/gene_synonym="IDDM12"
/db_xref="GeneID:1493"
/db_xref="HGNC:HGNC:2505"
/db_xref="MIM:123890"
CDS 157..828
/gene="CTLA4"
/gene_synonym="CD152"
/gene_synonym="CELIAC3"
/gene_synonym="CTLA-4"
/gene_synonym="GSE"
/gene_synonym="IDDM12"
/codon_start=1
/product="cytotoxic T-lymphocyte-associated protein 4"
/protein_id="AAH70162.1"
/db_xref="GeneID:1493"
/db_xref="HGNC:HGNC:2505"
/db_xref="MIM:123890"
/translation="MACLGFQRHKAQLNLAARTWPCTLLFFLLFIPVFCKAMHVAQPA
VVLASSRGIASFVCEYASPGKATEVRVTVLRQADSQVTEVCAATYMMGNELTFLDDSI
CTGTSSGNQVNLTIQGLRAMDTGLYICKVELMYPPPYYLGIGNGTQIYVIDPEPCPDS
DFLLWILAAVSSGLFFYSFLLTAVSLSKMLKKRSPLTTGVYVKMPPTEPECEKQFQPY
FIPIN"
BASE COUNT 250 a 228 c 203 g 233 t
ORIGIN
1 ccttctgtgt gtgcacatgt gtaatacata tctgggatca aagctatcta tataaagtcc
61 ttgattctgt gtgggttcaa acacatttca aagcttcagg atcctgaaag gttttgctct
121 acttcctgaa gacctgaaca ccgctcccat aaagccatgg cttgccttgg atttcagcgg
181 cacaaggctc agctgaacct ggctgccagg acctggccct gcactctcct gttttttctt
241 ctcttcatcc ctgtcttctg caaagcaatg cacgtggccc agcctgctgt ggtactggcc
301 agcagccgag gcatcgccag ctttgtgtgt gagtatgcat ctccaggcaa agccactgag
361 gtccgggtga cagtgcttcg gcaggctgac agccaggtga ctgaagtctg tgcggcaacc
421 tacatgatgg ggaatgagtt gaccttccta gatgattcca tctgcacggg cacctccagt
481 ggaaatcaag tgaacctcac tatccaagga ctgagggcca tggacacggg actctacatc
541 tgcaaggtgg agctcatgta cccaccgcca tactacctgg gcataggcaa cggaacccag
601 atttatgtaa ttgatccaga accgtgccca gattctgact tcctcctctg gatccttgca
661 gcagttagtt cggggttgtt tttttatagc tttctcctca cagctgtttc tttgagcaaa
721 atgctaaaga aaagaagccc tcttacaaca ggggtctatg tgaaaatgcc cccaacagag
781 ccagaatgtg aaaagcaatt tcagccttat tttattccca tcaattgaga aaccattatg
841 aagaagagag tccatatttc aatttccaag agctgaggca attcaaaaaa aaaaaaaaaa
901 aaaaaaaaaa aaaa
//