LOCUS BC070052 3431 bp mRNA linear HUM 17-JUL-2006
DEFINITION Homo sapiens WEE1 homolog (S. pombe), mRNA (cDNA clone MGC:87158
IMAGE:30336707), complete cds.
ACCESSION BC070052
VERSION BC070052.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 3431)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 3431)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (10-MAY-2004) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: Dr. Stefan Hansson
cDNA Library Preparation: Michael Brownstein / Ted Usdin
Laboratory
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Sequencing Group at the Stanford Human Genome
Center, Stanford University School of Medicine, Stanford, CA 94305
Web site: http://www-shgc.stanford.edu
Contact: (Dickson, Mark) mcd@paxil.stanford.edu
Dickson, M., Schmutz, J., Grimwood, J., Rodriquez, A., and Myers,
R. M.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 168 Row: j Column: 5
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 19718775.
FEATURES Location/Qualifiers
source 1..3431
/db_xref="H-InvDB:HIT000264010"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:87158 IMAGE:30336707"
/tissue_type="Placenta, pre-eclamptic"
/clone_lib="NIH_MGC_148"
/lab_host="DH10B"
/note="Vector: pBluescriptR"
gene 1..3431
/gene="WEE1"
/gene_synonym="WEE1hu"
/db_xref="GeneID:7465"
/db_xref="HGNC:HGNC:12761"
/db_xref="MIM:193525"
CDS 244..2184
/gene="WEE1"
/gene_synonym="WEE1hu"
/codon_start=1
/product="WEE1 homolog (S. pombe)"
/protein_id="AAH70052.1"
/db_xref="GeneID:7465"
/db_xref="HGNC:HGNC:12761"
/db_xref="MIM:193525"
/translation="MSFLSRQQPPPPRRAGAACTLRQKLIFSPCSDCEEEEEEEEEEG
SGHSTGEDSAFQEPDSPLPPARSPTEPGPERRRSPGPAPGSPGELEEDLLLPGACPGA
DEAGGGAEGDSWEEEGFGSSSPVKSPAAPYFLGSSFSPVRCGGPGDASPRGCGARRAG
EGRRSPRPDHPGTPPHKTFRKLRLFDTPHTPKSLLSKARGIDSSSVKLRGSSLFTDTE
KSGKREFDVRQTPQVNINPFTPDSLLLHSSGQCRRRKRTYWNDSCGEDMEASDYELED
ETRPAKRITITESNMKSRYTTEFHELEKIGSGEFGSVFKCVKRLDGCIYAIKRSKKPL
AGSVDEQNALREVYAHAVLGQHSHVVRYFSAWAEDDHMLIQNEYCNGGSLADAISENY
RIMSYFKEAELKDLLLQVGRGLRYIHSMSLVHMDIKPSNIFISRTSIPNAASEEGDED
DWASNKVMFKIGDLGHVTRISSPQVEEGDSRFLANEVLQENYTHLPKADIFALALTVV
CAAGAEPLPRNGDQWHEIRQGRLPRIPQVLSQEFTELLKVMIHPDPERRPSAMALVKH
SVLLSASRKSAEQLRIELNAEKFKNSLLQKELKKAQMAKAAAEERALFTDRMATRSTT
QSNRTSRLIGKKMNRSVSLTIY"
BASE COUNT 919 a 781 c 821 g 910 t
ORIGIN
1 acctgaggag acctcagcct cggtgctcgg gccgccccgc ctctgccgga aagtccgcgc
61 cgccgctgcc gccaccgtcc gcagcccgag cgccccggag ccgcaggccg ccgccgcgca
121 gagacgccgc ggctgcgact aggcgcgccc agccgcacgt ggcggacccg cccccaggcc
181 cgcagtgtcc tggaccccgc aggcctccgc tctcctgtcc tcggccccgt ccccagggcc
241 gcgatgagct tcctgagccg acagcagccg ccgccacccc gccgcgccgg ggcggcctgc
301 accttgcggc agaagctgat cttctcgccc tgcagcgact gtgaggagga ggaagaagag
361 gaggaggagg agggcagcgg ccacagcacc ggggaggact cggcctttca agagcccgac
421 tcgccgctgc cgcccgcgcg gagccccacg gagcccgggc ccgagcgccg ccgctcgccc
481 gggccggccc ccgggagccc cggcgagctg gaggaggacc tgttgctgcc cggcgcctgc
541 ccgggcgcgg acgaggcggg cggtggggcg gagggcgact cgtgggagga ggagggcttc
601 ggctcctcgt cgccggtcaa gtcgccggcg gccccctact tcctgggtag ctctttctcg
661 ccggtgcgct gcggcggccc aggagatgcg tcgccgcggg gttgcggggc gcgccgggcg
721 ggcgaaggcc gccgctcgcc gcggccggac cacccgggca ccccgccaca caagaccttc
781 cgcaagctgc gactcttcga caccccgcac acgcccaaga gtttgctctc caaagctcgg
841 ggaattgatt ccagctctgt taaactccgg ggtagttctc tcttcacgga tacagaaaaa
901 tcaggaaaaa gggaatttga tgtgcgacag actcctcaag tgaatattaa tccttttact
961 ccggattctt tgttgcttca ttcctcagga cagtgtcgtc gtagaaagag aacgtattgg
1021 aatgattcct gtggtgaaga catggaagcc agtgattatg agcttgaaga tgaaacaaga
1081 cctgctaaga gaattacaat tactgaaagc aatatgaagt cccggtatac aacagaattt
1141 catgagctag agaaaatcgg ctctggagaa tttggttctg tatttaagtg tgtgaagagg
1201 ctggatggat gcatttatgc cattaagcga tcaaaaaagc cattggcggg ctctgttgat
1261 gagcagaacg ctttgagaga agtatatgct catgcagtgc ttggacagca ttctcatgta
1321 gttcgatatt tctctgcgtg ggcagaagat gatcatatgc ttatacagaa tgaatattgt
1381 aatggtggaa gtttagctga tgctataagt gaaaactaca gaatcatgag ttactttaaa
1441 gaagcagagt tgaaggatct ccttttgcaa gttggccgag gcttgaggta tattcattca
1501 atgtctttgg ttcacatgga tataaaacct agtaatattt tcatatctcg aacctcaatc
1561 ccaaatgctg cctctgaaga aggagacgaa gatgattggg catccaacaa agttatgttt
1621 aaaataggtg atcttgggca tgtaacaagg atctccagtc cacaagttga agagggcgat
1681 agtcgttttc ttgcaaatga agttttacag gagaattata cccatctacc aaaagcagat
1741 atttttgcgc ttgccctcac agtggtatgt gctgctggtg ctgaacctct tccgagaaat
1801 ggagatcaat ggcatgaaat cagacagggt agattacctc ggataccaca agtgctttcc
1861 caagaattta cagagttgct aaaagttatg attcatccag atccagagag aagaccttca
1921 gcaatggcac tggtaaagca ttcagtattg ctgtccgctt ctagaaagag tgcagaacaa
1981 ttacgaatag aattgaatgc cgaaaagttc aaaaattcac ttttacaaaa agaactcaag
2041 aaagcacaga tggcaaaagc tgcagctgag gaaagagcac tcttcactga ccggatggcc
2101 actaggtcca ccacccagag taatagaaca tctcgactta ttggaaagaa aatgaaccgc
2161 tctgtcagcc ttactatata ctgagctact cctttcccac ctccccctga acactgtgac
2221 aagaggaagc taggttgaaa tcactgatag aatccagttt gcaattactt tctcgattgg
2281 tgtcagtagt tttactgatt aggactttta ttgtgaatta cagttgaaag ctgtattttg
2341 atgattgcta tgtcaggctt tcatctaatc ttaccagtct gtcttctgta ggatgtgtca
2401 ctgttggatg ttacaccagc ctttccaggg ttaaccactg tggtggtgtg ctgcttatag
2461 tttgctgttg cattgtaata aaaggtgtct ttccctgtag tgacctgtaa aaagtactca
2521 agggctttat tacagacata ccctcccttt gaaaagggac atgctaaaag actcattact
2581 actcagcctt caatgtacct gtgtgtccat cttatatttc tttttttttt aattgtgaat
2641 tagacttgta tatcccactg ggagcacttt gtaggcattg catgaaccat gggatgatga
2701 ttctgtggag gtattgcctt gtgaatttgc tgctatttta gttttgtctt tgctgtaaac
2761 ttgtagcatt aaacaatcat tgttgttaat aggtcttctt tttgaaacaa ttatgtgaaa
2821 tgtatagctg cttttgatga aaagcagcta tttgcctttt ttttttttcc tttgaacttt
2881 gaagctagtg cattggaaaa atgcaccctt tccctccttt ggaatgctgt attaatgtag
2941 tataataatt actggttttg taacttgttc tggtaatgtc cttcccggac tctttttaaa
3001 tgtctccccc taagttttat acttgattgt attattagtc tgtttttaaa tgttttgccc
3061 ggtttttctc ttcaatattt gtgtatataa accgatcttc gtgatactgt acatagctgt
3121 ttgaaatgcc agaatgactt ctgacattcc aagtttttca caaaatatat tttatctgtg
3181 attagccatt tgactaataa tactggctaa cagatgttga aaaaaattgt ctgtttgttt
3241 tctcattaat tttggtctaa aacatgtttg cacttgtctt tgacttgtgt tttattaaca
3301 ttgattggca tattaaaagt cactctgagc ttaaaaaaaa aaaaaaaaaa aaaaaaaaaa
3361 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
3421 aaaaaaaaaa a
//