LOCUS BC069228 2548 bp mRNA linear HUM 21-JUL-2006
DEFINITION Homo sapiens phosphatidylinositol glycan anchor biosynthesis, class
S, mRNA (cDNA clone MGC:78407 IMAGE:3944034), complete cds.
ACCESSION BC069228
VERSION BC069228.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2548)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 2548)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (26-APR-2004) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: DCTD/DTP
cDNA Library Preparation: Rubin Laboratory
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Genome Sequence Centre,
BC Cancer Agency, Vancouver, BC, Canada
info@bcgsc.bc.ca
Martin Hirst, Thomas Zeng, Ryan Morin, Michelle Moksa, Johnson
Pang, Diana Mah, Jing Wang, Kieth Fichter, Eric Chuah, Allen
Delaney, Rob Kirkpatrick, Agnes Baross, Sarah Barber, Mabel
Brown-John, Steve S. Chand, William Chow, Ryan Babakaiff, Dave
Wong, Corey Matsuo, Jaclyn Beland, Susan Gibson, Luis delRio, Ruth
Featherstone, Malachi Griffith, Obi Griffith, Ran Guin, Nancy Liao,
Kim MacDonald, Mike R. Mayo, Josh Moran, Diana Palmquist, JR
Santos, Duane Smailus, Jeff Stott, Miranda Tsai, George Yang,
Jacquie Schein, Asim Siddiqui,Steven Jones, Rob Holt, Marco Marra.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 54 Row: b Column: 15
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 23397651.
FEATURES Location/Qualifiers
source 1..2548
/db_xref="H-InvDB:HIT000263382"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:78407 IMAGE:3944034"
/tissue_type="Lung, small cell carcinoma"
/clone_lib="NIH_MGC_7"
/lab_host="DH10B-R"
/note="Vector: pOTB7"
gene 1..2548
/gene="PIGS"
/db_xref="GeneID:94005"
/db_xref="HGNC:HGNC:14937"
CDS 24..1691
/gene="PIGS"
/codon_start=1
/product="phosphatidylinositol glycan anchor biosynthesis,
class S"
/protein_id="AAH69228.1"
/db_xref="GeneID:94005"
/db_xref="HGNC:HGNC:14937"
/translation="MAAAGAAATHLEVARGKRAALFFAAVAIVLGLPLWWKTTETYRA
SLPYSQISGLNALQLRLMVPVTVVFTRESVPLDDQEKLPFTVVHEREIPLKYKMKIKC
RFQKAYRRALDHEEEALSSGSVQEAEAMLDEPQEQAEGSLTVYVISEHSSLLPQDMMS
YIGPKRTAVVRGIMHREAFNIIGRRIVQVAQAMSLTEDVLAAALADHLPEDKWSAEKR
RPLKSSLGYEITFSLLNPDPKSHDVYWDIEGAVRRYVQPFLNALGAAGNFSVDSQILY
YAMLGVNPRFDSASSSYYLDMHSLPHVINPVESRLGSSAASLYPVLNFLLYVPELAHS
PLYIQDKDGAPVATNAFHSPRWGGIMVYNVDSKTYNASVLPVRVEVDMVRVMEVFLAQ
LRLLFGIAQPQLPPKCLLSGPTSEGLMTWELDRLLWARSVENLATATTTLTSLAQLLG
KISNIVIKDDVASEVYKAVAAVQKSAEELASGHLASAFVASQEAVTSSELAFFDPSLL
HLLYFPDDQKFAIYIPLFLPMAVPILLSLVKIFLETRKSWRKPEKTD"
BASE COUNT 532 a 701 c 680 g 635 t
ORIGIN
1 ggccggagca gccggaagct agcatggcgg ccgccggggc tgcggctaca cacctagagg
61 tggcccgggg caagcgcgcc gccctcttct tcgctgcggt ggccatcgtg ctggggctac
121 cgctctggtg gaagaccacg gagacctacc gggcctcgtt gccttactcc cagatcagtg
181 gcctgaatgc ccttcagctc cgcctcatgg tgcctgtcac tgtcgtgttt acgcgggagt
241 cagtgcccct ggacgaccag gagaagctgc ccttcaccgt tgtgcatgaa agagagattc
301 ctctgaaata caaaatgaaa atcaaatgcc gtttccagaa ggcctatcgg agggctttgg
361 accatgagga ggaggccctg tcatcgggca gtgtgcaaga ggcagaagcc atgttagatg
421 agcctcagga acaagcggag ggctccctga ctgtgtacgt gatatctgaa cactcctcac
481 ttcttcccca ggacatgatg agctacattg ggcccaagag gacagcagtg gtgcggggga
541 taatgcaccg ggaggccttt aacatcattg gccgccgcat agtccaggtg gcccaggcca
601 tgtctttgac tgaggatgtg cttgctgctg ctctggctga ccaccttcca gaggacaagt
661 ggagcgctga gaagaggcgg cctctcaagt ccagcttggg ctatgagatc accttcagtt
721 tactcaaccc agaccccaag tcccatgatg tctactggga cattgagggg gctgtccggc
781 gctatgtgca acctttcctg aatgccctcg gtgccgctgg caacttctct gtggactctc
841 agattcttta ctatgcaatg ttgggggtga atccccgctt tgactcagct tcctccagct
901 actatttgga catgcacagc ctcccccatg tcatcaaccc agtggagtcc cggctgggat
961 ccagtgctgc ctccttgtac cctgtgctca actttctact ctacgtgcct gagcttgcac
1021 actcaccgct gtacattcag gacaaggatg gcgctccagt ggccaccaat gccttccata
1081 gtccccgctg gggtggcatt atggtatata atgttgactc caaaacctat aatgcctcag
1141 tgctgccagt gagagtcgag gtggacatgg tgcgagtgat ggaggtgttc ctggcacagt
1201 tgcggttgct ctttgggatt gctcagcccc agctgcctcc aaaatgcctg ctttcagggc
1261 ctacgagtga agggctaatg acctgggagc tagaccggct gctctgggct cggtcagtgg
1321 agaacctggc cacagccacc accaccctta cctccctggc gcagcttctg ggcaagatca
1381 gcaacattgt cattaaggac gacgtggcat ctgaggtgta caaggctgta gctgccgtcc
1441 agaagtcggc agaagagttg gcgtctgggc acctggcatc tgcctttgtc gccagccagg
1501 aagctgtgac atcctctgag cttgccttct ttgacccgtc actcctccac ctcctttatt
1561 tccctgatga ccagaagttt gccatctaca tcccactctt cctgcctatg gctgtgccca
1621 tcctcctgtc cctggtcaag atcttcctgg agacccgcaa gtcctggaga aagcctgaga
1681 agacagactg agcagggcag cacctccata ggaagccttc ctttctggcc aaggtgggcg
1741 gtgttagatt gtgaggcacg tacatggggc ctgccggaat gacttaaata tttgtctcca
1801 gtctccactg ttggctctcc agcaaccaaa gtacaacact ccaagatggg ttcatctttt
1861 cttcctttcc cattcacctg gctcaatcct cctccaccac caggggcctc aaaaggcaca
1921 tcatccgggt ctccttatct tgtttgataa ggctgctgcc tgtctccctc tgtggcaagg
1981 actgtttgtt cttttgcccc atttctcaac atagcacact tgtgcactga gaggagggag
2041 cattatggga aagtccctgc cttccacacc tctctctagt ccctgtggga cagccctagc
2101 ccctgctgtc atgaaggggc caggcattgg tcacctgtgg gaccttctcc ctcactcccc
2161 tccctcctag ttggctttgt ctgtcaggtg cagtctggcg ggagtccagg aggcagcagc
2221 tcaggacatg gtgctgtgtg tgtgtgtgtg tgtgtgtgtg tgtgtgtgtg tgtcagaggt
2281 tccagaaagt tccagatttg gaatcaaaca gtcctgaatt caaatccttg tttttgcact
2341 tattgtctgg agagctttgg ataaggtatt gaatctctct gagcctcagt ttttcatttg
2401 ttcaaatggc actgatgatg tctcccttac aagatggttg tgaggagtaa atgtgatcag
2461 catgtaaagt gtctggcgtg tagtaggctc ttaataaaca ctggctgaat atgaattgga
2521 atgataaaaa aaaaaaaaaa aaaaaaaa
//