LOCUS BC068976 5569 bp mRNA linear HUM 15-JUL-2006
DEFINITION Homo sapiens phospholipase D1, phosphatidylcholine-specific, mRNA
(cDNA clone MGC:70810 IMAGE:6068382), complete cds.
ACCESSION BC068976
VERSION BC068976.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 5569)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 5569)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (05-APR-2004) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC
cDNA Library Preparation: Life Technologies, Inc.
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: National Institutes of Health Intramural
Sequencing Center (NISC),
Gaithersburg, Maryland;
Web site: http://www.nisc.nih.gov/
Contact: nisc_mgc@nhgri.nih.gov
Akhter,N., Ayele,K., Beckstrom-Sternberg,S.M., Benjamin,B.,
Blakesley,R.W., Bouffard,G.G., Breen,K., Brinkley,C., Brooks,S.,
Dietrich,N.L., Granite,S., Guan,X., Gupta,J., Haghighi,P.,
Hansen,N., Ho,S.-L., Karlins,E., Kwong,P., Laric,P., Legaspi,R.,
Maduro,Q.L., Masiello,C., Maskeri,B., Mastrian,S.D.,McCloskey,J.C.,
McDowell,J., Pearson,R., Stantripop,S., Thomas,P.J., Touchman,J.W.,
Tsurgeon,C., Vogt,J.L., Walker,M.A., Wetherby,K.D., Wiggins,L.,
Young,A., Zhang,L.-H. and Green,E.D.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 140 Row: g Column: 4
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 46276864.
FEATURES Location/Qualifiers
source 1..5569
/db_xref="H-InvDB:HIT000263171"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:70810 IMAGE:6068382"
/tissue_type="Testis, embryonal carcinoma"
/clone_lib="NIH_MGC_92"
/lab_host="DH10B"
/note="Vector: pCMV-SPORT6"
gene 1..5569
/gene="PLD1"
/db_xref="GeneID:5337"
/db_xref="HGNC:HGNC:9067"
/db_xref="MIM:602382"
CDS 79..3303
/gene="PLD1"
/codon_start=1
/product="phospholipase D1, phosphatidylcholine-specific"
/protein_id="AAH68976.1"
/db_xref="GeneID:5337"
/db_xref="HGNC:HGNC:9067"
/db_xref="MIM:602382"
/translation="MSLKNEPRVNTSALQKIAADMSNIIENLDTRELHFEGEEVDYDV
SPSDPKIQEVYIPFSAIYNTQGFKEPNIQTYLSGCPIKAQVLEVERFTSTTRVPSINL
YTIELTHGEFKWQVKRKFKHFQEFHRELLKYKAFIRIPIPTRRHTFRRQNVREEPREM
PSLPRSSENMIREEQFLGRRKQLEDYLTKILKMPMYRNYHATTEFLDISQLSFIHDLG
PKGIEGMIMKRSGGHRIPGLNCCGQGRACYRWSKRWLIVKDSFLLYMKPDSGAIAFVL
LVDKEFKIKVGKKETETKYGIRIDNLSRTLILKCNSYRHARWWGGAIEEFIQKHGTNF
LKDHRFGSYAAIQENALAKWYVNAKGYFEDVANAMEEANEEIFITDWWLSPEIFLKRP
VVEGNRWRLDCILKRKAQQGVRIFIMLYKEVELALGINSEYTKRTLMRLHPNIKVMRH
PDHVSSTVYLWAHHEKLVIIDQSVAFVGGIDLAYGRWDDNEHRLTDVGSVKRVTSGPS
LGSLPPAAMESMESLRLKDKNEPVQNLPIQKSIDDVDSKLKGIGKPRKFSKFSLYKQL
HRHHLHDADSISSIDSTSSYFNHYRSHHNLIHGLKPHFKLFHPSSESEQGLTRPHADT
GSIRSLQTGVGELHGETRFWHGKDYCNFVFKDWVQLDKPFADFIDRYSTPRMPWHDIA
SAVHGKAARDVARHFIQRWNFTKIMKSKYRSLSYPFLLPKSQTTAHELRYQVPGSVHA
NVQLLRSAADWSAGIKYHEESIHAAYVHVIENSRHYIYIENQFFISCADDKVVFNKIG
DAIAQRILKAHRENQKYRVYVVIPLLPGFEGDISTGGGNALQAIMHFNYRTMCRGENS
ILGQLKAELGNQWINYISFCGLRTHAELEGNLVTELIYVHSKLLIADDNTVIIGSANI
NDRSMLGKRDSEMAVIVQDTETVPSVMDGKEYQAGRFARGLRLQCFRVVLGYLDDPSE
DIQDPVSDKFFKEVWVSTAARNATIYDKVFRCLPNDEVHNLIQLRDFINKPVLAKEDP
IRAEEELKKIRGFLVQFPFYFLSEESLLPSVGTKEAIVPMEVWT"
BASE COUNT 1707 a 1153 c 1205 g 1504 t
ORIGIN
1 gagtccgccg ccaacgcgca ggtgctagcg gccccttcgc cctgcagccc ctttgctttt
61 actctgtcca aagttaacat gtcactgaaa aacgagccac gggtaaatac ctctgcactg
121 cagaaaattg ctgctgacat gagtaatatc atagaaaatc tggacacgcg ggaactccac
181 tttgagggag aggaggtaga ctacgacgtg tctcccagcg atcccaagat acaagaagtg
241 tatatccctt tctctgctat ttataacact caaggattta aggagcctaa tatacagacg
301 tatctctccg gctgtccaat aaaagcacaa gttctggaag tggaacgctt cacatctaca
361 acaagggtac caagtattaa tctttacact attgaattaa cacatgggga atttaaatgg
421 caagttaaga ggaaattcaa gcattttcaa gaatttcaca gagagctgct caagtacaaa
481 gcctttatcc gcatccccat tcccactaga agacacacgt ttaggaggca aaacgtcaga
541 gaggagcctc gagagatgcc cagtttgccc cgttcatctg aaaacatgat aagagaagaa
601 caattccttg gtagaagaaa acaactggaa gattacttga caaagatact aaaaatgccc
661 atgtatagaa actatcatgc cacaacagag tttcttgata taagccagct gtctttcatc
721 catgatttgg gaccaaaggg catagaaggt atgataatga aaagatctgg aggacacaga
781 ataccaggct tgaattgctg tggtcaggga agagcctgct acagatggtc aaaaagatgg
841 ttaatagtga aagattcctt tttattgtat atgaaaccag acagcggtgc cattgccttc
901 gtcctgctgg tagacaaaga attcaaaatt aaggtgggga agaaggagac agaaacgaaa
961 tatggaatcc gaattgataa tctttcaagg acacttattt taaaatgcaa cagctataga
1021 catgctcggt ggtggggagg ggctatagaa gaattcatcc agaaacatgg caccaacttt
1081 ctcaaagatc atcgatttgg gtcatatgct gctatccaag agaatgcttt agctaaatgg
1141 tatgttaatg ccaaaggata ttttgaagat gtggcaaatg caatggaaga ggcaaatgaa
1201 gagattttta tcacagactg gtggctgagt ccagaaatct tcctgaaacg cccagtggtt
1261 gagggaaatc gttggaggtt ggactgcatt cttaaacgaa aagcacaaca aggagtgagg
1321 atcttcataa tgctctacaa agaggtggaa ctcgctcttg gcatcaatag tgaatacacc
1381 aagaggactt tgatgcgtct acatcccaac ataaaggtga tgagacaccc ggatcatgtg
1441 tcatccaccg tctatttgtg ggctcaccat gagaagcttg tcatcattga ccaatcggtg
1501 gcctttgtgg gagggattga cctggcctat ggaaggtggg acgacaatga gcacagactc
1561 acagacgtgg gcagtgtgaa gcgggtcact tcaggaccgt ctctgggttc cctcccacct
1621 gccgcaatgg agtctatgga atccttaaga ctcaaagata aaaatgagcc tgttcaaaac
1681 ctacccatcc agaagagtat tgatgatgtg gattcaaaac tgaaaggaat aggaaagcca
1741 agaaagttct ccaaatttag tctctacaag cagctccaca ggcaccacct gcacgacgca
1801 gatagcatca gcagcattga cagcacctcc agttatttta atcactatag aagtcatcac
1861 aatttaatcc atggtttaaa accccacttc aaactctttc acccgtccag tgagtctgag
1921 caaggactca ctagacctca tgctgatacc gggtccatcc gtagtttaca gacaggtgtg
1981 ggagagctgc atggggaaac cagattctgg catggaaagg actactgcaa tttcgtcttc
2041 aaagactggg ttcaacttga taaacctttt gctgatttca ttgacaggta ctccacgccc
2101 cggatgccct ggcatgacat tgcctctgca gtccacggga aggcggctcg tgatgtggca
2161 cgtcacttca tccagcgctg gaacttcaca aaaattatga aatcaaaata tcggtccctt
2221 tcttatcctt ttctgcttcc aaagtctcaa acaacagccc atgagttgag atatcaagtg
2281 cctgggtctg tccatgctaa cgtacagttg ctccgctctg ctgctgattg gtctgctggt
2341 ataaagtacc atgaagagtc catccacgcc gcttacgtcc atgtgataga gaacagcagg
2401 cactatatct atatcgaaaa ccagtttttc ataagctgtg ctgatgacaa agttgtgttc
2461 aacaagatag gcgatgccat tgcccagagg atcctgaaag ctcacaggga aaaccagaaa
2521 taccgggtat atgtcgtgat accacttctg ccagggttcg aaggagacat ttcaaccggc
2581 ggaggaaatg ctctacaggc aatcatgcac ttcaactaca gaaccatgtg cagaggagaa
2641 aattccatcc ttggacagtt aaaagcagag cttggtaatc agtggataaa ttacatatca
2701 ttctgtggtc ttagaacaca tgcagagctc gaaggaaacc tagtaactga gcttatctat
2761 gtccacagca agttgttaat tgctgatgat aacactgtta ttattggctc tgccaacata
2821 aatgaccgca gcatgctggg aaagcgtgac agtgaaatgg ctgtcattgt gcaagataca
2881 gagactgttc cttcagtaat ggatggaaaa gagtaccaag ctggccggtt tgcccgagga
2941 cttcggctac agtgctttag ggttgtcctt ggctatcttg atgacccaag tgaggacatt
3001 caggatccag tgagtgacaa attcttcaag gaggtgtggg tttcaacagc agctcgaaat
3061 gctacaattt atgacaaggt tttccggtgc cttcccaatg atgaagtaca caatttaatt
3121 cagctgagag actttataaa caagcccgta ttagctaagg aagatcccat tcgagctgag
3181 gaggaactga agaagatccg tggatttttg gtgcaattcc ccttttattt cttgtctgaa
3241 gaaagcctac tgccttctgt tgggaccaaa gaggccatag tgcccatgga ggtttggact
3301 taagagatat tcattggcag ctcaaagact tccaccctgg agaccacact gcacacagtg
3361 acttcctggg gatgtcatag ccaaagccag gcctgacgca ttctcgtatc caacccaagg
3421 accttttgga atgactgggg agggctgcag tcacattgat gtaaggactg taaacatcag
3481 caagacttta taattccttc tgcctaactt gtaaaaaggg ggctgcattc ttgttggtag
3541 catgtactct gttgagtaaa acacatattc aaattccgta taccaaaatc catttccttt
3601 gtaacaagaa tttaccagta actgtgatct aggttgccaa aagttgtctg aatctcctta
3661 ttcttctctg atcttcattt atgcagccaa tgtctagctg gacctgccct catcttgcag
3721 ttcatacagg cactgtttga gagattgttt attattagat gttgtaatgc tgcttcaaga
3781 ttcttcatgg ttacatggga tgcccctgct catctggtcc tgaagtagta acattcaccc
3841 aaatgaggat atagtcatta ttccttttca gtcactgtac gaacacggca gactttagcc
3901 tacacagtag actgtgttag gcacttctgg taacattgac actgtatctt gacaccacta
3961 agcaacagga aggaataaat tccaatattg agacagagat ttatttcatt ttgctcccaa
4021 aggcactgac aacatgagtc cttgtgatta ggcccacctg atcaaatgta aaaacatgca
4081 tgggatattt gattacgtaa caaccagcta aaactgagag cacacagcct tcggaaaccc
4141 ccaaggtgtc gaggaggatc agatccagat aagagaagat gattccttct agtcctgtaa
4201 aatttcctgt catccttagc tgctgggttg attgaaattt gatctctaca tttcaaaatc
4261 aaactagtga atacagtttg tctttcaaga gaatcagagg catcgtaact cctaccaagt
4321 tagtaagtat gagtctaacc ttgtttgaca ttcaggtctt ctgctacatt atcttctttg
4381 agaaaaatta attgattata aaatatgtgc tacttcactt acattattaa atatgtatta
4441 acgcctctca ccagggcttc cagtgaagtt acaatgccct agtctgtgaa ttagtctgga
4501 aacgtgtttt tccttttcgg atgttagagt accctttgat aaactaaatt ttactaagct
4561 gaacaactct gacagtctaa agagctaatg tgggttacca aaaggcctgt acctgtaaaa
4621 caaaatgcag gtgtaatgat tatacatgtc tatggattac ctggacatac tctcatttgg
4681 gttgttcttc aaagaagcaa gcagccgatc cctgttttca taaagctaat acttcagttg
4741 gaaaaattaa acaggagcac aaagtcaggg ataggggtta gcagaagaga gaaatagtgt
4801 cacatcaagg gcaggatctc atagctaggg aacatttcac aaataaggtg agattttgta
4861 accaataata aaaatgaatg tttttataag taaataactt atttttcata tggctaaaga
4921 tggtaaaatg acttcattct atagccattg taaataagaa tttgctattg atgaaagaag
4981 ttcagattgg catttgaagt attgagtgta tgggatctct aaggatttct tagattttat
5041 atttaaatat tttttaaacc ttagaggagt caacaaactg gctcttgatt ttcagcaccc
5101 tactctcatg aaaaaagcct gaaaggaccc tttcccttat aagtaattta atccaatttc
5161 tccccatttt atagatgagg aaactgaggc tcagatcaga tgagaactca cttaaatcca
5221 ctcaatgtgt agatggtaga gctgggacta gcaacattgc tgcagcccat tgttggcctc
5281 tctcttcact ttatcattgc ccaagaatga ggatatgcag taaacagaat tcaggcaaga
5341 tacctctaag ctgttttgaa ccctctgata ttttgtattt atgtgtttgt ctgtctcccc
5401 ctactagaat gtaagctcca tggggcaggg acttcactgt attttgttca tagtgtatcc
5461 ccagagcctg gaccagtgct tggcacatag gagatggcaa taaatgcttg tagaattaat
5521 aaacaaggtg aaggagagag ctaatgaaat gaaaaaaaaa aaaaaaaaa
//