LOCUS BC068005 3471 bp mRNA linear HUM 11-AUG-2006
DEFINITION Homo sapiens ligase III, DNA, ATP-dependent, mRNA (cDNA clone
MGC:75006 IMAGE:6092747), complete cds.
ACCESSION BC068005
VERSION BC068005.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 3471)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 3471)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (22-MAR-2004) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC
cDNA Library Preparation: Life Technologies, Inc.
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: National Institutes of Health Intramural
Sequencing Center (NISC),
Gaithersburg, Maryland;
Web site: http://www.nisc.nih.gov/
Contact: nisc_mgc@nhgri.nih.gov
Akhter,N., Ayele,K., Beckstrom-Sternberg,S.M., Benjamin,B.,
Blakesley,R.W., Bouffard,G.G., Breen,K., Brinkley,C., Brooks,S.,
Dietrich,N.L., Granite,S., Guan,X., Gupta,J., Haghighi,P.,
Hansen,N., Ho,S.-L., Karlins,E., Kwong,P., Laric,P., Legaspi,R.,
Maduro,Q.L., Masiello,C., Maskeri,B., Mastrian,S.D.,McCloskey,J.C.,
McDowell,J., Pearson,R., Stantripop,S., Thomas,P.J., Touchman,J.W.,
Tsurgeon,C., Vogt,J.L., Walker,M.A., Wetherby,K.D., Wiggins,L.,
Young,A., Zhang,L.-H. and Green,E.D.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 140 Row: g Column: 14
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 7710127.
FEATURES Location/Qualifiers
source 1..3471
/db_xref="H-InvDB:HIT000262880"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:75006 IMAGE:6092747"
/tissue_type="Pancreas, epithelioid carcinoma"
/clone_lib="NIH_MGC_70"
/lab_host="DH10B"
/note="Vector: pCMV-SPORT6"
gene 1..3471
/gene="LIG3"
/db_xref="GeneID:3980"
/db_xref="HGNC:HGNC:6600"
/db_xref="MIM:600940"
CDS 105..3134
/gene="LIG3"
/codon_start=1
/product="ligase III, DNA, ATP-dependent"
/protein_id="AAH68005.1"
/db_xref="GeneID:3980"
/db_xref="HGNC:HGNC:6600"
/db_xref="MIM:600940"
/translation="MSLAFKIFFPQTLRALSRKELCLFRKHHWRDVRQFSQWSETDLL
HGHPLFLRRKPVLSFQGSHLRSRATYLVFLPGLHVGLCSGPCEMAEQRFCVDYAKRGT
AGCKKCKEKIVKGVCRIGKVVPNPFSESGGDMKEWYHIKCMFEKLERARATTKKIEDL
TELEGWEELEDNEKEQITQHIADLSSKAAGTPKKKAVVQAKLTTTGQVTSPVKGASFV
TSTNPRKFSGFSAKPNNSGEAPSSPTPKRSLSSSKCDPRHKDCLLREFRKLCAMVADN
PSYNTKTQIIQDFLRKGSAGDGFHGDVYLTVKLLLPGVIKTVYNLNDKQIVKLFSRIF
NCNPDDMARDLEQGDVSETIRVFFEQSKSFPPAAKSLLTIQEVDEFLLRLSKLTKEDE
QQQALQDIASRCTANDLKCIIRLIKHDLKMNSGAKHVLDALDPNAYEAFKASRNLQDV
VERVLHNAQEVEKEPGQRRALSVQASLMTPVQPMLAEACKSVEYAMKKCPNGMFSEIK
YDGERVQVHKNGDHFSYFSRSLKPVLPHKVAHFKDYIPQAFPGGHSMILDSEVLLIDN
KTGKPLPFGTLGVHKKAAFQDANVCLFVFDCIYFNDVSLMDRPLCERRKFLHDNMVEI
PNRIMFSEMKRVTKALDLADMITRVIQEGLEGLVLKDVKGTYEPGKRHWLKVKKDYLN
EGAMADTADLVVLGAFYGQGSKGGMMSIFLMGCYDPGSQKWCTVTKCAGGHDDATLAR
LQNELDMVKISKDPSKIPSWLKVNKIYYPDFIVPDPKKAAVWEITGAEFSKSEAHTAD
GISIRFPRCTRIRDDKDWKSATNLPQLKELYQLSKEKADFTVVAGDEGSSTTGGSSEE
NKGPSGSAVSRKAPSKPSASTKKAEGKLSNSNSKDGNMQTAKPSAMKVGEKLATKSSP
VKVGEKRKAADETLCQTKVLLDIFTGVRLYLPPSTPDFSRLRRYFVAFDGDLVQEFDM
TSATHVLGSRDKNPAAQQVSPEWIWACIRKRRLVAPC"
BASE COUNT 932 a 858 c 921 g 760 t
ORIGIN
1 cggatttaaa gagacaggcg ctccaaccgt cgtgggctgc ccgcagcctg taatgagcaa
61 gttccgaggc ctacggtgag cgccggagcc ggagaggcag ctatatgtct ttggctttca
121 agatcttctt tccacaaacc ctccgtgcac tcagccgaaa agaactgtgc ctattccgaa
181 aacatcactg gcgtgatgta agacaattca gccagtggtc agaaacagat ctgcttcatg
241 gacatcccct cttcctgaga agaaagcctg ttctatcatt ccagggaagc catctaagat
301 cacgtgccac ctaccttgtt ttcttgccag ggttgcatgt gggactctgc agtggcccct
361 gtgagatggc tgagcaacgg ttctgtgtgg actatgccaa gcgtggcaca gctggctgca
421 aaaaatgcaa ggaaaagatt gtgaagggcg tatgccgaat tggcaaagtg gtgcccaatc
481 ccttctcaga gtctgggggt gatatgaaag agtggtacca cattaaatgc atgtttgaga
541 aactagagcg ggcccgggcc accacaaaaa aaatcgagga cctcacagag ctggaaggct
601 gggaagagct ggaagataat gagaaggaac agataaccca gcacattgca gatctgtctt
661 ctaaggcagc aggtacacca aagaagaaag ctgttgtcca ggctaagttg acaaccactg
721 gccaggtgac ttctccagtg aaaggcgcct catttgtcac cagtaccaat ccccggaaat
781 tttctggctt ttcagccaag cccaacaact ctggggaagc cccctcgagc cccaccccta
841 agagaagtct gtcttcaagc aaatgtgacc ccaggcataa ggactgtctg ctacgggagt
901 ttcgaaagtt atgcgccatg gtggccgata atcctagcta caacacgaag acccagatca
961 tccaggactt ccttcggaaa ggctcagcag gagatggttt ccacggtgat gtgtacctaa
1021 cagtgaagct gctgctgcca ggagtcatta agactgttta caacttgaac gataagcaga
1081 ttgtgaagct tttcagtcgc atttttaact gcaacccaga tgatatggca cgggacctag
1141 agcagggtga cgtgtcagag acaatcagag tcttctttga gcagagcaag tctttccccc
1201 cagctgccaa gagcctcctt accatccagg aagtggatga gttccttctg cggctgtcca
1261 agctcaccaa ggaggatgag cagcaacagg ccctacagga cattgcctcc aggtgtacag
1321 ccaatgacct taaatgcatc atcaggttga tcaaacatga tctgaagatg aactcaggtg
1381 caaaacatgt gttagacgcc cttgacccca atgcctatga agccttcaaa gcctcgcgca
1441 acctgcagga tgtggtggag cgggtccttc acaacgcgca ggaggtggag aaggagccgg
1501 gccagagacg agctctgagc gtccaggcct cgctgatgac acctgtgcag cccatgttgg
1561 cggaggcctg caagtccgtt gagtatgcaa tgaagaaatg tcccaatggc atgttctctg
1621 agatcaagta cgatggagag cgagtccagg tgcataagaa tggagaccac ttcagctact
1681 tcagccgcag tctcaagccc gtccttcctc acaaggtggc ccactttaag gactacattc
1741 cccaggcttt tcctgggggc cacagcatga tcttggattc tgaagtgctt ctgattgaca
1801 acaagacagg caaaccactg ccctttggga ctctgggagt acacaagaaa gcagccttcc
1861 aggatgctaa tgtctgcctg tttgtttttg attgtatcta ctttaatgat gtcagcttga
1921 tggacagacc tctgtgtgag cggcggaagt ttcttcatga caacatggtt gaaattccaa
1981 accggatcat gttctcagaa atgaagcgag tcacaaaagc tttggacttg gctgacatga
2041 taacccgggt gatccaggag ggattggagg ggctggtgct gaaggatgtg aagggtacat
2101 atgagcctgg gaagcggcac tggctgaaag tgaagaaaga ctatttgaac gagggggcca
2161 tggccgacac agctgacctg gtggtccttg gagccttcta tgggcaaggg agcaaaggcg
2221 gcatgatgtc aatcttcctc atgggctgct acgaccctgg cagccagaag tggtgcacag
2281 tcaccaagtg tgcaggaggc catgatgatg ccacgcttgc ccgcctgcag aatgaactag
2341 acatggtgaa gatcagcaag gaccccagca aaatacccag ctggttgaag gtcaacaaga
2401 tctactatcc tgacttcatc gtcccagacc caaagaaagc tgccgtgtgg gagatcacag
2461 gggctgaatt ctccaaatcg gaggctcata cagctgacgg gatctccatc cgattccctc
2521 gctgcacccg aatccgagat gataaggact ggaaatctgc cactaacctt ccccaactca
2581 aggaactgta ccagttgtcc aaggagaagg cagacttcac tgtagtggct ggagatgagg
2641 ggagctccac tacagggggt agcagtgaag agaataaggg tccctcaggg tctgctgtgt
2701 cccgcaaggc ccccagcaag ccctcagcca gtaccaagaa agcagaaggg aagctgagta
2761 actccaacag caaagatggc aacatgcaga ctgcaaagcc ttccgctatg aaggtggggg
2821 agaagctggc cacaaagtct tctccagtga aagtagggga gaagcggaaa gctgctgatg
2881 agacgctgtg ccaaacaaag gtattgctgg acatcttcac tggggtgcgg ctttacttgc
2941 caccctccac accagacttc agccgtctca gacgctactt tgtggcattc gacggggacc
3001 tggtacagga atttgatatg acttcagcca cgcacgtgct gggtagcagg gacaagaacc
3061 ctgcggccca gcaggtctcc ccagagtgga tttgggcatg tatccggaaa cggagactgg
3121 tagctccctg ctaggtttgc tgtcttccct ctccctcagg ccatactctc ctttaccata
3181 ctattggact ggactcaggc tggaggcaga tagacacagt atagggggaa tgggcttgct
3241 tctcccaaac ccaccagttc tccactgtct cttctggacc aggaattagt tgctgtgggt
3301 gccacagctg aagtcagttt gtcttgctgg tttaaataga tctttcagag ctgggtgctg
3361 ggtttgccat ctttttgttt tctttgaaaa gcagcttagt tacccttttt ataaataaaa
3421 tatcttgcag ttaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa a
//