LOCUS BC067298 2379 bp mRNA linear HUM 30-MAR-2004
DEFINITION Homo sapiens RAS guanyl releasing protein 1 (calcium and
DAG-regulated), mRNA (cDNA clone IMAGE:30377671), partial cds.
ACCESSION BC067298
VERSION BC067298.1
KEYWORDS .
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2379)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 2379)
AUTHORS Strausberg,R.
TITLE Direct Submission
JOURNAL Submitted (12-MAR-2004) National Institutes of Health, Mammalian
Gene Collection (MGC), Cancer Genomics Office, National Cancer
Institute, 31 Center Drive, Room 11A03, Bethesda, MD 20892-2590,
USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: Dr. Michael Brownstein
cDNA Library Preparation: Invitrogen Corp
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Genome Sequence Centre,
BC Cancer Agency, Vancouver, BC, Canada
info@bcgsc.bc.ca
Steve Jones, Sarah Barber, Mabel Brown-John, Yaron Butterfield,
Andy Chan, Steve S. Chand, William Chow, Alison Cloutier, Ruth
Featherstone, Malachi Griffith, Obi Griffith, Ran Guin, Nancy Liao,
Kim MacDonald, Amara Masson, Mike R. Mayo, Josh Moran, Ryan Morin,
Teika Olson, Diana Palmquist, Anca Petrescu, Anna Liisa Prahbu,
Parvaneh Saeedi, JR Santos, Angelique Schnerch, Ursula Skalska,
Duane Smailus, Jeff Stott, Miranda Tsai, George Yang, Jacquie
Schein, Asim Siddiqui, Rob Holt, Marco Marra.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 141 Row: m Column: 3
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 6382080.
FEATURES Location/Qualifiers
source 1..2379
/db_xref="H-InvDB:HIT000262543"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="IMAGE:30377671"
/tissue_type="Testis"
/clone_lib="NIH_MGC_180"
/lab_host="DH10B"
/note="Vector: pCMV-SPORT6.1"
gene 1..>2379
/gene="RASGRP1"
/gene_synonym="CALDAG-GEFI"
/gene_synonym="RASGRP"
/gene_synonym="V"
/db_xref="GeneID:10125"
/db_xref="MIM:603962"
CDS 84..>2379
/gene="RASGRP1"
/gene_synonym="CALDAG-GEFI"
/gene_synonym="RASGRP"
/gene_synonym="V"
/codon_start=1
/product="RASGRP1 protein"
/protein_id="AAH67298.1"
/db_xref="GeneID:10125"
/db_xref="MIM:603962"
/translation="MGTLGKAREAPRKPSHGCRAASKARLEAKPANSPFPSHPSLAHI
TQFRMMVSLGHLAKGASLDDLIDSCIQSFDADGNLCRSNQLLQVMLTMHRIVISSAEL
LQKVITLYKDALAKNSPGLCLKICYFVRYWITEFWVMFKMDASLTDTMEEFQELVKAK
GEELHCRLIDTTQINARDWSRKLTQRIKSNTSKKRKVSLLFDHLEPEELSEHLTYLEF
KSFRRISFSDYQNYLVNSCVKENPTMERSIALCNGISQWVQLMVLSRPTPQLRAEVFI
KFIQVAQKLHQLQNFNTLMAVIGGLCHSSISRLKETSSHVPHEINKVLGEMTELLSSS
RNYDNYRRAYGECTDFKIPILGVHLKDLISLYEAMPDYLEDGKVNVHKLLALYNHISE
LVQLQEVAPPLEANKDLVHLLTLSLDLYYTEDEIYELSYAREPRNHRAPPLTPSKPPV
VVDWASGVSPKPDPKTISKHVQRMVDSVFKNYDHDQDGYISQEEFEKIAASFPFSFCV
MDKDREGLISRDEITAYFMRASSIYSKLGLGFPHNFQETTYLKPTFCDNCAGFLWGVI
KQGYRCKDCGMNCHKQCKDLVVFECKKRAKNPVAPTENNTSVGPVSNLCSLGAKDLLH
APEEGPFTFPNGEAVEHGEESKDRTIMLMGVSSQKISLRLKRAVAHKATQTESQPWIG
SEGPSGPFVLSSPRKTAQDTLYVLPSPTSPCPSPVLVRKRAFVKWENKDSLIKSKEEK
KKKKKKKKKKKKKKKKKKKKKKKKK"
misc_feature 243..608
/gene="RASGRP1"
/gene_synonym="CALDAG-GEFI"
/gene_synonym="RASGRP"
/gene_synonym="V"
/note="RasGEFN; Region: Guanine nucleotide exchange factor
for Ras-like GTPases"
/db_xref="CDD:smart00229"
misc_feature 684..1373
/gene="RASGRP1"
/gene_synonym="CALDAG-GEFI"
/gene_synonym="RASGRP"
/gene_synonym="V"
/note="RasGEF; Region: Guanine nucleotide exchange factor
for Ras-like small GTPases. Small GTP-binding proteins of
the Ras superfamily function as molecular switches in
fundamental events such as signal transduction,
cytoskeleton dynamics and intracellular trafficking.
Guanine-nucleotide-exchange factors (GEFs) positively
regulate these GTP-binding proteins in response to a
variety of signals. GEFs catalyze the dissociation of GDP
from the inactive GTP-binding proteins. GTP can then bind
and induce structural changes that allow interaction with
effectors"
/db_xref="CDD:cd00155"
misc_feature 1503..1664
/gene="RASGRP1"
/gene_synonym="CALDAG-GEFI"
/gene_synonym="RASGRP"
/gene_synonym="V"
/note="EFh; Region: EF-hand, calcium binding motif"
/db_xref="CDD:cd00051"
misc_feature 1707..1856
/gene="RASGRP1"
/gene_synonym="CALDAG-GEFI"
/gene_synonym="RASGRP"
/gene_synonym="V"
/note="DAG_PE-bind; Region: Phorbol esters/diacylglycerol
binding domain (C1 domain). This domain is also known as
the Protein kinase C conserved region 1 (C1) domain"
/db_xref="CDD:pfam00130"
BASE COUNT 689 a 599 c 571 g 520 t
ORIGIN
1 caggcggcgg cggtggtggc gggggcgatg cgccgcgccc ggccgcgcta ggtgagccgg
61 caccgggagc gcgggccgcg gccatgggca ccctgggcaa ggcgagagag gctccgcgga
121 aaccttccca tggctgcaga gctgcctcta aagcaagact agaggcaaag ccagccaaca
181 gccccttccc ctcccatccc agcttggccc acatcaccca gttccgaatg atggtgtctc
241 tgggacattt agccaaagga gccagcctgg acgatctcat tgacagctgc attcaatctt
301 ttgatgcaga tggaaacctg tgtcgaagta accaactgtt gcaagtcatg ctgaccatgc
361 accgaattgt catctcctct gcagaactgc tccaaaaagt tatcaccctc tataaggatg
421 ctttggcaaa gaattcacca ggactttgcc tgaagatctg ttattttgta aggtattgga
481 taacagaatt ctgggtcatg tttaaaatgg acgccagctt gacagacact atggaggagt
541 ttcaggaact ggtgaaagct aagggtgagg agttacattg ccgcctgatt gacacaactc
601 aaatcaatgc ccgtgactgg tccaggaaac ttactcaaag gataaaatca aataccagca
661 agaaacggaa agtctccctg ctctttgacc atctggaacc agaagagcta tccgagcacc
721 tcacctacct tgagttcaag tctttccgga ggatatcgtt ctctgattat cagaattacc
781 ttgtaaatag ctgtgtgaag gaaaacccca ccatggagcg atctattgct ctgtgcaacg
841 gcatctccca gtgggtacaa ctgatggttc tcagccgccc cacgccgcag ctccgagcag
901 aagtcttcat caagttcatc caggtggctc agaagctcca ccaactacag aacttcaata
961 cactgatggc tgtgataggt gggctgtgtc acagctcaat ctcgaggctc aaggagacaa
1021 gttcgcatgt cccacatgaa atcaataagg ttctcggtga gatgactgag ctgctgtcct
1081 cctccagaaa ctacgacaat taccggcgag cctatggaga gtgcaccgac ttcaagatcc
1141 ccattctggg tgtgcatctc aaggacctca tctccctgta tgaagccatg cctgactatc
1201 tggaggacgg gaaagtgaac gtccataagc tactggccct atacaatcat atcagtgaat
1261 tggtccagct gcaagaggtg gccccaccct tggaggctaa caaggacttg gtacacttgc
1321 tgacgttatc cctggatctt tactacactg aggatgaaat ctatgagctt tcctatgccc
1381 gggaaccaag gaaccacaga gctccaccac taacaccttc aaagccacca gtagtagtgg
1441 actgggcttc tggagtgtct cccaaacctg atccaaaaac cattagcaaa cacgtccaga
1501 ggatggtgga ttctgtcttc aagaactatg atcacgacca ggatggatac atttctcagg
1561 aagaattcga aaagattgct gcgagttttc cattttcctt ctgtgtgatg gacaaagaca
1621 gggaaggcct catcagcagg gatgagatca cagcctactt catgagagcc agctcaatct
1681 attccaagct gggcctgggc tttcctcaca acttccaaga gaccacctac ctgaagccca
1741 ctttttgtga caactgtgct ggatttctct ggggagtgat caaacaagga tatcgatgta
1801 aagactgcgg gatgaactgt cacaaacaat gcaaagatct ggttgtgttt gagtgtaaga
1861 agcgagccaa gaacccagta gctcccacag agaacaacac ttctgtgggg ccagtgtcca
1921 acctttgctc attgggagcc aaagatctgc tccatgcacc tgaggaagga ccttttacat
1981 tccctaatgg ggaggctgtg gaacatggtg aggagagtaa ggatcggacc atcatgctga
2041 tgggagtgtc ctcacagaag atttctcttc ggctgaagag ggctgttgcc cacaaggcca
2101 cccagactga atcacagcct tggattggca gtgagggccc ttcaggtccc tttgtgctgt
2161 cttccccaag gaagacagcc caggatactc tatatgtgct tcccagtccc acctctccat
2221 gtcctagccc agtcttggtc agaaagcggg cttttgtcaa gtgggagaat aaagactccc
2281 tcataaaatc aaaggaggaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
2341 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaa
//