LOCUS BC067257 1593 bp mRNA linear HUM 19-MAR-2009
DEFINITION Homo sapiens alkB, alkylation repair homolog 3 (E. coli), mRNA
(cDNA clone MGC:74669 IMAGE:6176933), complete cds.
ACCESSION BC067257
VERSION BC067257.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1593)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 1593)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (12-MAR-2004) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: Dr. James R. Lupski
cDNA Library Preparation: Life Technologies, Inc.
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Genome Sequence Centre,
BC Cancer Agency, Vancouver, BC, Canada
info@bcgsc.bc.ca
Martin Hirst, Thomas Zeng, Ryan Morin, Michelle Moksa, Johnson
Pang, Diana Mah, Jing Wang, Kieth Fichter, Eric Chuah, Allen
Delaney, Rob Kirkpatrick, Agnes Baross, Sarah Barber, Mabel
Brown-John, Steve S. Chand, William Chow, Ryan Babakaiff, Dave
Wong, Corey Matsuo, Jaclyn Beland, Susan Gibson, Luis delRio, Ruth
Featherstone, Malachi Griffith, Obi Griffith, Ran Guin, Nancy Liao,
Kim MacDonald, Mike R. Mayo, Josh Moran, Diana Palmquist, JR
Santos, Duane Smailus, Jeff Stott, Miranda Tsai, George Yang,
Jacquie Schein, Asim Siddiqui,Steven Jones, Rob Holt, Marco Marra.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 141 Row: a Column: 14
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 21040274
The stop codon of the CDS annotated on this record is located > 55
bases upstream of a splice junction, and therefore the mRNA is
predicted to be subject to nonsense-mediated mRNA decay (NMD).
FEATURES Location/Qualifiers
source 1..1593
/db_xref="H-InvDB:HIT000262502"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:74669 IMAGE:6176933"
/tissue_type="Peripheral Nervous System, dorsal root
ganglion"
/clone_lib="Lupski_dorsal_root_ganglion"
/lab_host="DH10B"
/note="Vector: pCMV-SPORT6"
gene 1..1593
/gene="ALKBH3"
/gene_synonym="ABH3"
/gene_synonym="DEPC-1"
/gene_synonym="DEPC1"
/gene_synonym="PCA1"
/db_xref="GeneID:221120"
/db_xref="HGNC:HGNC:30141"
/db_xref="MIM:610603"
CDS 377..889
/gene="ALKBH3"
/gene_synonym="ABH3"
/gene_synonym="DEPC-1"
/gene_synonym="DEPC1"
/gene_synonym="PCA1"
/codon_start=1
/product="ALKBH3 protein"
/protein_id="AAH67257.1"
/db_xref="GeneID:221120"
/db_xref="HGNC:HGNC:30141"
/db_xref="MIM:610603"
/translation="MEEKRRRARVQGAWAAPVKSQAIAQPATTAKSHLHQKPGQTWKN
KEHHLSDREFVFKEPQQVVRRAPEPRVIEEGVYEISLSPTGVSRVCLYPGFVDVKEAD
WILEQLCQDVPWKQRTGIREDSILQLTFKKSAPVSGTATAPQSCWYERPSPPHIPGPA
ILTRTRLWAP"
BASE COUNT 449 a 393 c 407 g 344 t
ORIGIN
1 tgcgagggct gccccaagtc ctaccgggtt tgcacgggcg cgcccggctc cgcccgcaag
61 tgcgccttcc tgacttactg ctgggtgcgc ggggctgggg gtgcgagtac cacccctgaa
121 gtctcttcct gggcgacctc cggggcctca ttctaggcct ccttaaagag aaggatctaa
181 attaggaaaa ggaagtgccc ttatccacga ccaagctctt ccacctgcgg agctcgctta
241 gtctgcacct caaccgtgcg gaaagtgact gccctgttta ctgaggaaaa actggggctc
301 agaaagatac catggagtag tttgaaacag gaacaaaatc ttctgaaagc tcggagcaga
361 agcctttttg gtcaacatgg aggaaaaaag acggcgagcc cgagttcagg gagcctgggc
421 tgcccctgtt aaaagccagg ccattgctca gccagctacc actgctaaga gccatctcca
481 ccagaagcct ggccagacct ggaagaacaa agagcatcat ctctctgaca gagagtttgt
541 gttcaaagaa cctcagcagg tagtacgtag agctcctgag ccacgagtga ttgaagaggg
601 tgtgtatgaa atcagcctgt cacccacagg tgtatctagg gtctgtttgt atcctggctt
661 tgttgacgtg aaagaagctg actggatatt ggaacagctt tgtcaagatg ttccctggaa
721 acagaggacc ggcatcagag aggattccat attgcaacta acctttaaga agtcagcacc
781 tgttagtgga accgcgactg ctccgcagag ctgctggtat gagcgcccgt cgccacccca
841 catcccaggc ccagccattc tgacaagaac tagactttgg gctccttgag aatattcagt
901 ttttggcacc ctgtgctgcg cacactaaag aaccgcattg aagagaacac tggccacacc
961 ttcaactcct tactctgcaa tctttatcgc aatgagaagg acagcgtgga ctggcacagt
1021 gatgatgaac cctcactagg gaggtgcccc attattgctt cactaagttt tggtgccaca
1081 cgcacatttg agatgagaaa gaagccacca ccaagaatgg agactacaca tatgtggaaa
1141 gagtgaagat acccttggat catggtacct tgttaatcat ggaaggagcg acacaagctg
1201 actggcagca tcgagtgccc aaagaatacc actctagaga accgagagtg aacctgacct
1261 ttcggacagt ctatccagac cctcgagggg caccctggtg acgtcagagc tttgagagag
1321 aagcttcact gaaacggagc aaaccttcca ctgagaagcc acttcaagag gctggtgctg
1381 ctagatctca tgatgtggct gttgggaaga tggtggggtt tgtttgccag cttggagtcc
1441 tattaaatga aagccagcaa ctcatgttgg taataggtct actgtgggaa cagttatccc
1501 taaccacagc tcaaaatcgc tatcatcttt aggcaaatta aaatctatgt ggcagtgaaa
1561 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaa
//