LOCUS BC066980 1939 bp mRNA linear HUM 18-MAR-2009
DEFINITION Homo sapiens chromosome 5 open reading frame 25, mRNA (cDNA clone
MGC:87612 IMAGE:4821958), complete cds.
ACCESSION BC066980
VERSION BC066980.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1939)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 1939)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (01-MAR-2004) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: Miklos Palkovits, M.D., Ph.D.
cDNA Library Preparation: Michael J. Brownstein (NHGRI) & Shiraki
Toshiyuki and Piero Carninci (RIKEN)
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Sequencing Group at the Stanford Human Genome
Center, Stanford University School of Medicine, Stanford, CA 94305
Web site: http://www-shgc.stanford.edu
Contact: (Dickson, Mark) mcd@paxil.stanford.edu
Dickson, M., Schmutz, J., Grimwood, J., Rodriquez, A., and Myers,
R. M.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 167 Row: e Column: 23
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 38348409
The stop codon of the CDS annotated on this record is located > 55
bases upstream of a splice junction, and therefore the mRNA is
predicted to be subject to nonsense-mediated mRNA decay (NMD).
FEATURES Location/Qualifiers
source 1..1939
/db_xref="H-InvDB:HIT000262400"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:87612 IMAGE:4821958"
/tissue_type="Testis"
/clone_lib="NIH_MGC_97"
/lab_host="DH10B"
/note="Vector: pBluescriptR"
gene 1..1939
/gene="C5orf25"
/db_xref="GeneID:375484"
/db_xref="HGNC:HGNC:24779"
CDS 144..1517
/gene="C5orf25"
/codon_start=1
/product="C5orf25 protein"
/protein_id="AAH66980.1"
/db_xref="GeneID:375484"
/db_xref="HGNC:HGNC:24779"
/translation="MEDFIVISDDSGSESSGGARPGRSRRPRRALSRTSGALPRRTVN
KGQKLEPIPHRRLRMVTNTIEENFPLGTVQFLMDFVSPQHYPPREIVAHIIQKILLSG
SETVDVLKEAYMLLMKIQQLHPANAKTVEWDWKLLTYVMEEEGQTLPGRVLFLRYVVQ
TLEDDFQQTLRRQRQHLQQSIANMVLSCDKQPHNVRDVIKWLVKAVTEDGLTQPPNGN
QTSSGTGILKASSSHPSSQPNLTKNTNQLIVCQLQRMLSIAVEVDRTPTCSSNKIAEM
MLGFVLDIPERSQREMFFTTMESHLLRCKVLEIIFLHSCETPTRLPLSLAQALYFLNN
STSLLKCQSDKSQWQTWDELVEHLQFLLSSYQHVLREHLRSSVIDRKDLIIKRIKPKP
QQGDDITVVDVEKQIEAFRSRLIQMLGEPLVPQLQDKVHLLKLLLFYAADLNPDAEPF
QKGWSGS"
BASE COUNT 526 a 507 c 472 g 434 t
ORIGIN
1 gctcggggcg gggccggcgt ggcggtggcc cgggttgtag ccgccgccgc tgccgccgcc
61 cccgccgccg ccacctcaga agcaggaacc gaagtcgtcg ccgccgccgc cactgcctgg
121 gtcttcggtc ggggcccgca gccatggagg atttcatcgt gatctcggac gacagcggct
181 ctgagagctc cgggggcgcc cgcccgggcc ggtcgcggag gccgcgccgg gccctgtcgc
241 gaacctccgg cgcgctgccc cgccggaccg tgaacaaggg tcaaaaatta gaacccatcc
301 ctcatcgaag actaagaatg gtaacaaata ccattgaaga gaattttcct ctggggactg
361 tgcagttttt gatggacttt gtgtcacccc agcattaccc accaagagaa atcgtggctc
421 acatcatcca gaaaatcttg ctcagtggct ctgagactgt ggatgtccta aaggaggcct
481 acatgcttct catgaaaatt caacagctac atccagccaa tgccaagaca gtggagtggg
541 actggaaact gctcacctat gtcatggagg aagagggaca aactctgcct gggcgagtcc
601 ttttcctgcg ttatgtcgtt cagaccctag aagatgactt tcagcagacc ctgaggaggc
661 aacggcagca cctgcagcaa tccattgcaa acatggtgct ttcctgtgac aagcagcccc
721 acaatgtcag ggatgttatc aagtggctgg tcaaagcagt aactgaagat ggattgactc
781 agcccccaaa tggaaatcaa acgtcttcag gaacaggaat cttgaaagcc agcagtagcc
841 acccttcttc ccagcccaac ctgacaaaga acaccaatca gctgattgtg tgccagcttc
901 agaggatgct ctccatagcc gtagaggtgg acaggacccc cacctgcagc tccaataaaa
961 ttgccgagat gatgttgggg tttgtgctgg acattcctga gaggagccag agagaaatgt
1021 tctttactac catggaaagc caccttctgc gctgcaaagt gttagaaatc atattcctcc
1081 acagctgtga gacacccacc cgcctgcctc tgtctctggc ccaggccctc tactttctga
1141 ataattctac gtcactgctc aagtgtcagt cagataaaag ccagtggcag acttgggacg
1201 aattggttga gcatctgcag tttctgctgt ccagttatca acatgtttta agagaacact
1261 taaggagttc cgtgatcgac cgaaaggact taataatcaa aaggattaag cccaaacccc
1321 agcaaggaga tgacatcaca gtggtagacg tagagaagca gattgaggcc ttccgcagcc
1381 gcctgatcca gatgctgggg gagcctcttg tcccccaact ccaagacaaa gtgcacttgt
1441 tgaagctcct gctcttctat gctgcggact tgaaccctga tgcagagccc tttcaaaagg
1501 gctggagcgg ctcctgaggg cctgccaagc actgaatgcc aagaatacct cctgaactct
1561 ctctccaact gctcagaagc tctaaaagca tgaaaagtgg ttaaaatctt acaggaccaa
1621 acctgcatta tttaatcagt taatactctg tgcatcacat gtttaacatt ttcattcaag
1681 atgtggaaaa aatccctctg ctgaacctag tctatacacc aatattatgt cattcaaggt
1741 accgacaacc tgtttcagga gagagacgtt catttttccc taatgaaatg caagcattct
1801 gttagaccta ttatattgcc tgttaatttg actgtaatga atagggggta gaaacaaaag
1861 gatcaagtgt gttataaaac atttgatgtt aaaaggagac aataaaaagg caatggtttt
1921 tccaaaaaaa aaaaaaaaa
//