LOCUS BC066953 1391 bp mRNA linear HUM 19-MAR-2007
DEFINITION Homo sapiens pyruvate dehydrogenase (lipoamide) alpha 2, mRNA (cDNA
clone MGC:87402 IMAGE:5273609), complete cds.
ACCESSION BC066953
VERSION BC066953.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1391)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 1391)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (01-MAR-2004) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: Miklos Palkovits, M.D., Ph.D.
cDNA Library Preparation: Michael J. Brownstein (NHGRI) & Shiraki
Toshiyuki and Piero Carninci (RIKEN)
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Sequencing Group at the Stanford Human Genome
Center, Stanford University School of Medicine, Stanford, CA 94305
Web site: http://www-shgc.stanford.edu
Contact: (Dickson, Mark) mcd@paxil.stanford.edu
Dickson, M., Schmutz, J., Grimwood, J., Rodriquez, A., and Myers,
R. M.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 167 Row: o Column: 14
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 38492354.
FEATURES Location/Qualifiers
source 1..1391
/db_xref="H-InvDB:HIT000262373"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:87402 IMAGE:5273609"
/tissue_type="Testis"
/clone_lib="NIH_MGC_97"
/lab_host="DH10B"
/note="Vector: pBluescriptR"
gene 1..1391
/gene="PDHA2"
/db_xref="GeneID:5161"
/db_xref="HGNC:HGNC:8807"
/db_xref="MIM:179061"
CDS 46..1212
/gene="PDHA2"
/codon_start=1
/product="pyruvate dehydrogenase (lipoamide) alpha 2"
/protein_id="AAH66953.2"
/db_xref="GeneID:5161"
/db_xref="HGNC:HGNC:8807"
/db_xref="MIM:179061"
/translation="MLAAFISRVLRRVAQKSARRVLVASRNSSNDATFEIKKCDLYLL
EEGPPVTTVLTRAEGLKYYRMMLTVRRMELKADQLYKQKFIRGFCHLCDGQEACCVGL
EAGINPSDHVITSYRAHGVCYTRGPSVRSILAELTGRRGGCAKGKGGSMHMYTKNFYG
GNGIVGAQGPLGAGIALACKYKGNDEICLTLYGDGAANQGQIAEAFNMAALWKLPCVF
ICENNLYGMGTSTERAAASPDYYKRGNFIPGLKVDGMDVLCVREATKFAANYCRSGKG
PILMELQTYRYHGHSMSDPGVSYRTREEIQEVRSKRDPIIILQDRMVNSKLATVEELK
EIGAEVRKEIDDAAQFATTDPEPHLEELGHHIYSSDSSFEVRGANPWIKFKSVS"
BASE COUNT 413 a 272 c 360 g 346 t
ORIGIN
1 gtcgttacgg gacgccgctg ccatctacag cactccgtga agaatatgct ggccgccttc
61 atctcccgcg tgttgaggcg agttgcccag aaatcagctc gcagagtgct ggtggcatcc
121 cgtaactcct caaatgacgc tacatttgaa attaagaaat gtgatcttta tctgttggaa
181 gagggtcccc ctgtcactac agtgctcact agggcggagg ggcttaaata ctacaggatg
241 atgctgactg ttcgccgcat ggaattgaag gcagatcagc tgtacaaaca gaaattcatt
301 cgcggtttct gtcacctgtg cgatggtcag gaagcttgtt gcgtgggcct tgaggccggc
361 ataaacccct cggatcacgt cattacatcc tatagggctc atggtgtgtg ctatactcgg
421 ggaccttctg tccgatccat tctcgcagag ctgacgggaa gaagaggagg ttgtgctaaa
481 ggaaaaggag gatcgatgca tatgtatacc aagaacttct atgggggcaa tggcatcgtc
541 ggtgcacagg gccccctggg cgctggcatt gctctggcct gtaaatataa aggaaacgat
601 gagatctgtt tgactttata tggggatggc gctgcgaatc aggggcagat agccgaagct
661 ttcaatatgg cagctttatg gaaattacct tgtgttttca tctgtgagaa taacctatat
721 ggaatgggaa catctactga gagagcagca gccagccctg attactacaa gaggggcaat
781 tttatccctg ggctaaaggt cgatggaatg gatgttctgt gtgttcgtga ggcaacaaaa
841 tttgcagcta actactgtag atctggaaag gggcccatac tgatggagct gcaaacctac
901 cgttatcatg gacacagtat gagtgatcct ggagtcagtt atcgtacacg agaagaaatt
961 caggaagtaa gaagtaagag ggatcctata ataattctcc aagatagaat ggtaaacagc
1021 aagctcgcca ctgtggaaga attaaaggaa attggggctg aggtgaggaa agaaattgat
1081 gatgctgccc agtttgctac cactgatcct gagccacatt tggaagaatt aggccatcac
1141 atctacagca gtgattcatc ttttgaagtt cgtggtgcaa atccatggat caagtttaag
1201 tccgtcagtt aaagggaggc tacgtgtgaa tttatcatca gtctctcaat ggaatgttca
1261 tggtcaaatt taagaaactg tgttctcaac tcaaggagga ataaaactca taaaacaaaa
1321 gccttgtaag catttattaa aagagattat taaaagagat tgaaaaaaaa aaaagaaaaa
1381 aaagaaaaaa a
//