LOCUS BC066656 2764 bp mRNA linear HUM 15-JUL-2006
DEFINITION Homo sapiens heat shock protein 90kDa beta (Grp94), member 1, mRNA
(cDNA clone MGC:75130 IMAGE:6165138), complete cds.
ACCESSION BC066656
VERSION BC066656.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2764)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 2764)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (01-MAR-2004) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC/DCTD/DTP
cDNA Library Preparation: Life Technologies, Inc.
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: National Institutes of Health Intramural
Sequencing Center (NISC),
Gaithersburg, Maryland;
Web site: http://www.nisc.nih.gov/
Contact: nisc_mgc@nhgri.nih.gov
Akhter,N., Ayele,K., Beckstrom-Sternberg,S.M., Benjamin,B.,
Blakesley,R.W., Bouffard,G.G., Breen,K., Brinkley,C., Brooks,S.,
Dietrich,N.L., Granite,S., Guan,X., Gupta,J., Haghighi,P.,
Hansen,N., Ho,S.-L., Karlins,E., Kwong,P., Laric,P., Legaspi,R.,
Maduro,Q.L., Masiello,C., Maskeri,B., Mastrian,S.D.,McCloskey,J.C.,
McDowell,J., Pearson,R., Stantripop,S., Thomas,P.J., Touchman,J.W.,
Tsurgeon,C., Vogt,J.L., Walker,M.A., Wetherby,K.D., Wiggins,L.,
Young,A., Zhang,L.-H. and Green,E.D.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 140 Row: n Column: 8
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 4507676.
FEATURES Location/Qualifiers
source 1..2764
/db_xref="H-InvDB:HIT000262289"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:75130 IMAGE:6165138"
/tissue_type="Skin, melanotic melanoma."
/clone_lib="NIH_MGC_72"
/lab_host="DH10B"
/note="Vector: pCMV-SPORT6"
gene 1..2764
/gene="HSP90B1"
/gene_synonym="ECGP"
/gene_synonym="GP96"
/gene_synonym="GRP94"
/db_xref="GeneID:7184"
/db_xref="HGNC:HGNC:12028"
/db_xref="MIM:191175"
CDS 97..2508
/gene="HSP90B1"
/gene_synonym="ECGP"
/gene_synonym="GP96"
/gene_synonym="GRP94"
/codon_start=1
/product="heat shock protein 90kDa beta (Grp94), member 1"
/protein_id="AAH66656.1"
/db_xref="GeneID:7184"
/db_xref="HGNC:HGNC:12028"
/db_xref="MIM:191175"
/translation="MRALWVLGLCCVLLTFGSVRADDEVDVDGTVEEDLGKSREGSRT
DDEVVQREEEAIQLDGLNASQIRELREKSEKFAFQAEVNRMMKLIINSLYKNKEIFLR
ELISNASDALDKIRLISLTDENALSGNEELTVKIKCDKEKNLLHVTDTGVGMTREELV
KNLGTIAKSGTSEFLNKMTEAQEDGQSTSELIGQFGVGFYSAFLVADKVIVTSKHNND
TQHIWESDSNEFSVIADPRGNTLGRGTTITLVLKEEASDYLELDTIKNLVKKYSQFIN
FPIYVWSSKTETVEEPMEEEEAAKEEKEESDDEAAVEEEEEEKKPKTKKVEKTVWDWE
LMNDIKPIWQRPSKEVEEDEYKAFYKSFSKESDDPMAYIHFTAEGEVTFKSILFVPTS
APRGLFDEYGSKKSDYIKLYVRRVFITDDFHDMMPKYLNFVKGVVDSDDLPLNVSRET
LQQHKLLKVIRKKLVRKTLDMIKKIADDKYNDTFWKEFGTNIKLGVIEDHSNRTRLAK
LLRFQSSHHPTDITSLDQYVERMKEKQDKIYFMAGSSRKEAESSPFVERLLKKGYEVI
YLTEPVDEYCIQALPEFDGKRFQNVAKEGVKFDESEKTKESREAVEKEFEPLLNWMKD
KALKDKIEKAVVSQRLTESPCALVASQYGWSGNMERIMKAQAYQTGKDISTNYYASQK
KTFEINPRHPLIRDMLRRIKEDEDDKTVLDLAVVLFETATLRSGYLLPDTKAYGDRIE
RMLRLSLNIDPDAKVEEEPEEEPEETAEDTTEDTEQDEDEEMDVGTDEEEETAKESTA
EKDEL"
BASE COUNT 930 a 487 c 663 g 684 t
ORIGIN
1 ccgcgcggct ggaggtgtga ggatccgaac ccaggggtgg ggggtggagg cggctcctgc
61 gatcgaaggg gacttgagac tcaccggccg cacgccatga gggccctgtg ggtgctgggc
121 ctctgctgcg tcctgctgac cttcgggtcg gtcagagctg acgatgaagt tgatgtggat
181 ggtacagtag aagaggatct gggtaaaagt agagaaggat caaggacgga tgatgaagta
241 gtacagagag aggaagaagc tattcagttg gatggattaa atgcatcaca aataagagaa
301 cttagagaga agtcggaaaa gtttgccttc caagccgaag ttaacagaat gatgaaactt
361 atcatcaatt cattgtataa aaataaagag attttcctga gagaactgat ttcaaatgct
421 tctgatgctt tagataagat aaggctaata tcactgactg atgaaaatgc tctttctgga
481 aatgaggaac taacagtcaa aattaagtgt gataaggaga agaacctgct gcatgtcaca
541 gacaccggtg taggaatgac cagagaagag ttggttaaaa accttggtac catagccaaa
601 tctgggacaa gcgagttttt aaacaaaatg actgaagcac aggaagatgg ccagtcaact
661 tctgaattga ttggccagtt tggtgtcggt ttctattccg ccttccttgt agcagataag
721 gttattgtca cttcaaaaca caacaacgat acccagcaca tctgggagtc tgactccaat
781 gaattttctg taattgctga cccaagagga aacactctag gacggggaac gacaattacc
841 cttgtcttaa aagaagaagc atctgattac cttgaattgg atacaattaa aaatctcgtc
901 aaaaaatatt cacagttcat aaactttcct atttatgtat ggagcagcaa gactgaaact
961 gttgaggagc ccatggagga agaagaagca gccaaagaag agaaagaaga atctgatgat
1021 gaagctgcag tagaggaaga agaagaagaa aagaaaccaa agactaaaaa agttgaaaaa
1081 actgtctggg actgggaact tatgaatgat atcaaaccaa tatggcagag accatcaaaa
1141 gaagtagaag aagatgaata caaagctttc tacaaatcat tttcaaagga aagtgatgac
1201 cccatggctt atattcactt tactgctgaa ggggaagtta ccttcaaatc aattttattt
1261 gtacccacat ctgctccacg tggtctgttt gacgaatatg gatctaaaaa gagcgattac
1321 attaagctct atgtgcgccg tgtattcatc acagacgact tccatgatat gatgcctaaa
1381 tacctcaatt ttgtcaaggg tgtggtggac tcagatgatc tccccttgaa tgtttcccgc
1441 gagactcttc agcaacataa actgcttaag gtgattagga agaagcttgt tcgtaaaacg
1501 ctggacatga tcaagaagat tgctgatgat aaatacaatg atactttttg gaaagaattt
1561 ggtaccaaca tcaagcttgg tgtgattgaa gaccactcga atcgaacacg tcttgctaaa
1621 cttcttaggt tccagtcttc tcatcatcca actgacatta ctagcctaga ccagtatgtg
1681 gaaagaatga aggaaaaaca agacaaaatc tacttcatgg ctgggtccag cagaaaagag
1741 gctgaatctt ctccatttgt tgagcgactt ctgaaaaagg gctatgaagt tatttacctc
1801 acagaacctg tggatgaata ctgtattcag gcccttcccg aatttgatgg gaagaggttc
1861 cagaatgttg ccaaggaagg agtgaagttc gatgaaagtg agaaaactaa ggagagtcgt
1921 gaagcagttg agaaagaatt tgagcctctg ctgaattgga tgaaagataa agcccttaag
1981 gacaagattg aaaaggctgt ggtgtctcag cgcctgacag aatctccgtg tgctttggtg
2041 gccagccagt acggatggtc tggcaacatg gagagaatca tgaaagcaca agcgtaccaa
2101 acgggcaagg acatctctac aaattactat gcgagtcaga agaaaacatt tgaaattaat
2161 cccagacacc cgctgatcag agacatgctt cgacgaatta aggaagatga agatgataaa
2221 acagttttgg atcttgctgt ggttttgttt gaaacagcaa cgcttcggtc agggtatctt
2281 ttaccagaca ctaaagcata tggagataga atagaaagaa tgcttcgcct cagtttgaac
2341 attgaccctg atgcaaaggt ggaagaagag cctgaagaag aacctgaaga gacagcagaa
2401 gacacaacag aagacacaga gcaagacgaa gatgaagaaa tggatgtggg aacagatgaa
2461 gaagaagaaa cagcaaagga atctacagct gaaaaagatg aattgtaaat tatactctca
2521 ccatttggat cctgtgtgga gagggaatgt gaaatttaca tcatttcttt ttgggagaga
2581 cttgttttgg atgcccccta atccccttct cccctgcact gtaaaatgtg ggattatggg
2641 tcacaggaaa aagtgggttt tttagttgaa ttttttttaa cattcctcat gaatgtaaat
2701 ttgtactatt taactgacta ttcttgatgt aaaatcttgt catgtgtata aaaaaaaaaa
2761 aaaa
//