LOCUS BC066331 789 bp mRNA linear HUM 02-MAR-2004 DEFINITION Homo sapiens coiled-coil-helix-coiled-coil-helix domain containing 2, mRNA (cDNA clone MGC:87328 IMAGE:4792941), complete cds. ACCESSION BC066331 VERSION BC066331.1 KEYWORDS MGC. SOURCE Homo sapiens (human) ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 789) AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G., Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D., Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K., Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F., Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L., Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L., Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S., Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J., Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J., McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S., Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W., Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A., Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S., Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y., Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D., Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M., Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E., Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A. TITLE Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002) PUBMED 12477932 REFERENCE 2 (bases 1 to 789) AUTHORS Strausberg,R. TITLE Direct Submission JOURNAL Submitted (03-FEB-2004) National Institutes of Health, Mammalian Gene Collection (MGC), Cancer Genomics Office, National Cancer Institute, 31 Center Drive, Room 11A03, Bethesda, MD 20892-2590, USA REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov COMMENT Contact: MGC help desk Email: cgapbs-r@mail.nih.gov Tissue Procurement: Miklos Palkovits, M.D., Ph.D. cDNA Library Preparation: Michael J. Brownstein (NHGRI) & Shiraki Toshiyuki and Piero Carninci (RIKEN) cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL) DNA Sequencing by: Sequencing Group at the Stanford Human Genome Center, Stanford University School of Medicine, Stanford, CA 94305 Web site: http://www-shgc.stanford.edu Contact: (Dickson, Mark) mcd@paxil.stanford.edu Dickson, M., Schmutz, J., Grimwood, J., Rodriquez, A., and Myers, R. M. Clone distribution: MGC clone distribution information can be found through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov Series: IRAK Plate: 167 Row: b Column: 7 This clone was selected for full length sequencing because it passed the following selection criteria: matched mRNA gi: 7705850. FEATURES Location/Qualifiers source 1..789 /db_xref="H-InvDB:HIT000262222" /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="MGC:87328 IMAGE:4792941" /tissue_type="Brain, hypothalamus" /clone_lib="NIH_MGC_96" /lab_host="DH10B" /note="Vector: pBluescript" gene 1..789 /gene="CHCHD2" /gene_synonym="C7orf17" /db_xref="GeneID:51142" CDS 41..496 /gene="CHCHD2" /gene_synonym="C7orf17" /codon_start=1 /product="CHCHD2 protein" /protein_id="AAH66331.1" /db_xref="GeneID:51142" /translation="MPRGSRSRTSRMAPPASRAPQMRAAPRPAPVAQPPAAAPPSAVG SSAAAPRQPVLMAQMATTAAGVAVGSAVGHTLGHAITGGFSGGSNAEPARPDITYQEP QGTQPAQQQQPCLYEIKQFLECAQNQGDIKLCEGFNEVLKQCRLANGLA" misc_feature 380..481 /gene="CHCHD2" /gene_synonym="C7orf17" /note="CHCH; Region: CHCH domain. we have identified a conserved motif in the LOC118487 protein that we have called the CHCH motif. Alignment of this protein with related members showed the presence of three subgroups of proteins, which are called the S (Small), N (N-terminal extended) and C (C-terminal extended) subgroups. All three sub-groups of proteins have in common that they contain a predicted conserved [coiled coil 1]-[helix 1]-[coiled coil 2]-[helix 2] domain (CHCH domain). Within each helix of the CHCH domain, there are two cysteines present in a C-X9-C motif. The N-group contains an additional double helix domain, and each helix contains the C-X9-C motif. This family contains a number of characterised proteins: Cox19 protein - a nuclear gene of Saccharomyces cerevisiae, codes for an 11-kDa protein (Cox19p) required for expression of cytochrome oxidase. Because cox19 mutants are able to synthesise the mitochondrial and nuclear gene products of cytochrome oxidase, Cox19p probably functions post-translationally during assembly of the enzyme. Cox19p is present in the cytoplasm and mitochondria, where it exists as a soluble intermembrane protein. This dual location is similar to what was previously reported for Cox17p, a low molecular weight copper protein thought to be required for maturation of the CuA centre of subunit 2 of cytochrome oxidase. Cox19p have four conserved potential metal ligands, these are three cysteines and one histidine. Mrp10 - belongs to the class of yeast mitochondrial ribosomal proteins that are essential for translation. Eukaryotic NADH-ubiquinone oxidoreductase 19 kDa (NDUFA8) subunit" /db_xref="CDD:pfam06747" BASE COUNT 198 a 201 c 207 g 183 t ORIGIN 1 gttgtcacac gtccggaggc ctagccgtcg cgtacctagg atgccgcgtg gaagccgaag 61 ccgcacctcc cgcatggccc ctccggccag ccgggcccct cagatgagag ctgcacccag 121 gccagcacca gtcgctcagc caccagcagc ggcaccccca tctgcagttg gctcttctgc 181 tgctgcgccc cggcagccag ttctgatggc ccagatggca accactgcag ctggcgtggc 241 tgtgggctct gctgtggggc acacattggg tcacgccatt actgggggct tcagtggagg 301 aagtaatgct gagcctgcga ggcctgacat cacttaccag gagcctcagg gaacccagcc 361 agcacagcag cagcagcctt gcctctatga gatcaaacag tttctggagt gtgcccagaa 421 ccagggtgac atcaagctct gtgagggttt caatgaggtg ctgaaacagt gccgacttgc 481 aaacggattg gcctaatgaa gaagttcaac ctggagagat ggaaaatcag ctctcataac 541 taagttaatt tagtataaaa atagaattga tagtgagggt ataaagtgta accatcagtt 601 aaacctctcc tgtcattcct agcttccttg cttcagaatt gaaatggaag tgggtgtccc 661 tactctgtag aatctgggac tgggcaaatg tttgtgtggc ctccttaaac tagctgttat 721 gttatgattt tattctttgt gagttaatta gaataaagtc attttcttcc aaggaaaaaa 781 aaaaaaaaa //