LOCUS BC065816 1978 bp mRNA linear HUM 03-FEB-2004
DEFINITION Homo sapiens LIM and senescent cell antigen-like domains 2, mRNA
(cDNA clone MGC:75000 IMAGE:6181259), complete cds.
ACCESSION BC065816
VERSION BC065816.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1978)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 1978)
AUTHORS Strausberg,R.
TITLE Direct Submission
JOURNAL Submitted (26-JAN-2004) National Institutes of Health, Mammalian
Gene Collection (MGC), Cancer Genomics Office, National Cancer
Institute, 31 Center Drive, Room 11A03, Bethesda, MD 20892-2590,
USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: Dr. James R. Lupski
cDNA Library Preparation: Life Technologies, Inc.
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Genome Sequence Centre,
BC Cancer Agency, Vancouver, BC, Canada
info@bcgsc.bc.ca
Steve Jones, Sarah Barber, Mabel Brown-John, Yaron Butterfield,
Andy Chan, Steve S. Chand, William Chow, Alison Cloutier, Ruth
Featherstone, Malachi Griffith, Obi Griffith, Ran Guin, Nancy Liao,
Kim MacDonald, Amara Masson, Mike R. Mayo, Josh Moran, Ryan Morin,
Teika Olson, Diana Palmquist, Anca Petrescu, Anna Liisa Prahbu,
Parvaneh Saeedi, JR Santos, Angelique Schnerch, Ursula Skalska,
Duane Smailus, Jeff Stott, Miranda Tsai, George Yang, Jacquie
Schein, Asim Siddiqui, Rob Holt, Marco Marra.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 141 Row: c Column: 4
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 8922203.
FEATURES Location/Qualifiers
source 1..1978
/db_xref="H-InvDB:HIT000262056"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:75000 IMAGE:6181259"
/tissue_type="Peripheral Nervous System, dorsal root
ganglion"
/clone_lib="Lupski_dorsal_root_ganglion"
/lab_host="DH10B"
/note="Vector: pCMV-SPORT6"
gene 1..1978
/gene="LIMS2"
/gene_synonym="FLJ10044"
/gene_synonym="PINCH-2"
/db_xref="GeneID:55679"
/db_xref="MIM:607908"
CDS 85..1110
/gene="LIMS2"
/gene_synonym="FLJ10044"
/gene_synonym="PINCH-2"
/codon_start=1
/product="LIMS2 protein"
/protein_id="AAH65816.1"
/db_xref="GeneID:55679"
/db_xref="MIM:607908"
/translation="MTGSNMSDALANAVCQRCQARFSPAERIVNSNGELYHEHCFVCA
QCFRPFPEGLFYEFEGRKYCEHDFQMLFAPCCGSCGEFIIGRVIKAMNNNWHPGCFRC
ELCDVELADLGFVKNAGRHLCRPCHNREKAKGLGKYICQRCHLVIDEQPLMFRSDAYH
PDHFNCTHCGKELTAEARELKGELYCLPCHDKMGVPICGACRRPIEGRVVNALGKQWH
VEHFVCAKCEKPFLGHRHYEKKGLAYCETHYNQLFGDVCYNCSHVIEGDVVSALNKAW
CVSCFSCSTCNSKLTLKNKFVEFDMKPVCKRCYEKFPLELKKRLKKLSELTSRKAQPK
ATDLNSA"
misc_feature 127..303
/gene="LIMS2"
/gene_synonym="FLJ10044"
/gene_synonym="PINCH-2"
/note="LIM; Region: LIM domain. This family represents two
copies of the LIM structural domain"
/db_xref="CDD:pfam00412"
misc_feature 310..480
/gene="LIMS2"
/gene_synonym="FLJ10044"
/gene_synonym="PINCH-2"
/note="LIM; Region: LIM domain. This family represents two
copies of the LIM structural domain"
/db_xref="CDD:pfam00412"
misc_feature 502..663
/gene="LIMS2"
/gene_synonym="FLJ10044"
/gene_synonym="PINCH-2"
/note="LIM; Region: LIM domain. This family represents two
copies of the LIM structural domain"
/db_xref="CDD:pfam00412"
misc_feature 676..846
/gene="LIMS2"
/gene_synonym="FLJ10044"
/gene_synonym="PINCH-2"
/note="LIM; Region: LIM domain. This family represents two
copies of the LIM structural domain"
/db_xref="CDD:pfam00412"
misc_feature 853..1023
/gene="LIMS2"
/gene_synonym="FLJ10044"
/gene_synonym="PINCH-2"
/note="LIM; Region: LIM domain. This family represents two
copies of the LIM structural domain"
/db_xref="CDD:pfam00412"
BASE COUNT 359 a 669 c 563 g 387 t
ORIGIN
1 gggcgggcgc tcggctggcg gctgcagcag cagagggaga cccgcggcaa ccccggcaac
61 ccagggctcg gcgtcgctgc caccatgacg ggaagcaata tgtcggacgc cttggccaac
121 gccgtgtgcc agcgctgcca ggcccgcttc tcccccgccg agcgcattgt caacagcaat
181 ggggagctgt accatgagca ctgcttcgtg tgtgcccagt gcttccggcc cttccccgag
241 gggctcttct atgagtttga aggccggaag tactgcgaac acgacttcca aatgctgttt
301 gctccgtgct gtggatcctg cggtgagttc atcattggcc gcgtcatcaa ggccatgaac
361 aacaactggc acccgggctg cttccgctgc gagctgtgtg atgtggagct ggctgacctg
421 ggctttgtga agaatgccgg caggcatctc tgccggcctt gccacaaccg tgagaaggcc
481 aaaggcctgg gcaagtacat ctgccagcgg tgccacctgg tcatcgacga gcagcccctc
541 atgttcagga gcgacgccta ccaccctgac cacttcaact gcacccactg tgggaaggag
601 ctgacagccg aggcccgcga gctgaagggt gagctctact gcctgccctg ccatgacaag
661 atgggcgtcc ccatctgcgg ggcctgccgc cggcccatcg agggccgagt ggtcaacgcg
721 ctgggcaagc agtggcacgt ggagcacttt gtctgtgcca agtgtgagaa gccattcctg
781 gggcaccggc actatgagaa gaagggcctg gcctactgcg agactcacta caaccagctc
841 ttcggggacg tctgctacaa ctgcagccat gtgattgaag gcgatgtggt gtcggccctc
901 aacaaggcct ggtgtgtgag ctgcttctcc tgctccacct gcaacagcaa gctcaccctg
961 aagaacaagt ttgtggagtt cgacatgaag cccgtgtgta agaggtgcta cgagaagttc
1021 ccgctggagc tgaagaagcg gctgaagaag ctgtcggagc tgacctcccg caaggcccag
1081 cccaaggcca cagacctcaa ctctgcctga aggccctctt gcgcagctgc ctctcggccc
1141 ctccgccttc tcccctcctg ctgtccatgc ttggccccct cgtccccatc cacctgtgcc
1201 ctccgcatct taccctccct ttctctttcc tcattgcctt ctcccttcct gttccctcat
1261 ctctgccttc cccatgtctc tcctctcctt ggccgtggct tctgtctgtg aggaggcagg
1321 agctggggag tgggagccta tgaccccacg tctgacagcc atgtccacct gtgcccacag
1381 cttccgccca cagacctcca gggacaggag caaattgcac cacagctccc cgcctggcct
1441 ggccctcccc aggcggctca gtggctcatg ctgtcctgtg agagcccctg ccccagagcg
1501 gccccactaa gcgcatgtgg ctcctgggct acccacagcc agggcagcct gctggagcca
1561 cagggccagg gccatgcaga tggaggcctc tgggagccac ctccaatccc tcaccactca
1621 ctcaaccagt ggcacagtgt ccttgtgccc acactgagcc agcaagtcct gctgtccaca
1681 cccacaagct acctggaggg acaggaccca cctccatcct tcggaaggcc ttcctggaat
1741 cccaccttgg cctccgccct cggttccgcc ccgcccctct ccccccgacc ttggggcttg
1801 tgtcgagccc ttgggtgggg ccaggaggag gtgatggcgt cagaggaggt gtggtcagag
1861 gtgacttgtt cccacctcca gggaggacgc ttcgtcttcg gccagcgcag acctggtgtt
1921 tgtttgttgg gctcacgctt gcacaatgaa ggcttgttca cacaaaaaaa aaaaaaaa
//