LOCUS BC065761 1071 bp mRNA linear HUM 12-FEB-2004
DEFINITION Homo sapiens eukaryotic translation elongation factor 1 alpha 1,
mRNA (cDNA clone IMAGE:4663222), partial cds.
ACCESSION BC065761
VERSION BC065761.1
KEYWORDS .
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1071)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 1071)
AUTHORS Strausberg,R.
TITLE Direct Submission
JOURNAL Submitted (26-JAN-2004) National Institutes of Health, Mammalian
Gene Collection (MGC), Cancer Genomics Office, National Cancer
Institute, 31 Center Drive, Room 11A03, Bethesda, MD 20892-2590,
USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: CLONTECH
cDNA Library Preparation: CLONTECH Laboratories, Inc.
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Genome Sequence Centre,
BC Cancer Agency, Vancouver, BC, Canada
info@bcgsc.bc.ca
Steve Jones, Sarah Barber, Mabel Brown-John, Yaron Butterfield,
Andy Chan, Steve S. Chand, William Chow, Alison Cloutier, Ruth
Featherstone, Malachi Griffith, Obi Griffith, Ran Guin, Nancy Liao,
Kim MacDonald, Amara Masson, Mike R. Mayo, Josh Moran, Ryan Morin,
Teika Olson, Diana Palmquist, Anca Petrescu, Anna Liisa Prahbu,
Parvaneh Saeedi, JR Santos, Angelique Schnerch, Ursula Skalska,
Duane Smailus, Jeff Stott, Miranda Tsai, George Yang, Jacquie
Schein, Asim Siddiqui, Rob Holt, Marco Marra.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 51 Row: h Column: 6.
FEATURES Location/Qualifiers
source 1..1071
/db_xref="H-InvDB:HIT000262042"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="IMAGE:4663222"
/tissue_type="Lung"
/clone_lib="NIH_MGC_77"
/lab_host="DH10B"
/note="Vector: pDNR-LIB"
gene <1..1071
/gene="EEF1A1"
/gene_synonym="EEF-1"
/gene_synonym="EEF1A"
/gene_synonym="eEF1A-1"
/gene_synonym="EEF1A1L14"
/gene_synonym="EF-Tu"
/gene_synonym="EF1A"
/gene_synonym="GRAF-1EF"
/gene_synonym="MGC16224"
/gene_synonym="PTI-1"
/gene_synonym="PTI1"
/db_xref="GeneID:1915"
/db_xref="MIM:130590"
CDS <1..757
/gene="EEF1A1"
/gene_synonym="EEF-1"
/gene_synonym="EEF1A"
/gene_synonym="eEF1A-1"
/gene_synonym="EEF1A1L14"
/gene_synonym="EF-Tu"
/gene_synonym="EF1A"
/gene_synonym="GRAF-1EF"
/gene_synonym="MGC16224"
/gene_synonym="PTI-1"
/gene_synonym="PTI1"
/codon_start=2
/product="EEF1A1 protein"
/protein_id="AAH65761.1"
/db_xref="GeneID:1915"
/db_xref="MIM:130590"
/translation="GGWKVTRKDGNASGTTLLEALDCILPPTRPTDKPLRLPLQDVYK
IGGIGTVPVGRVETGVLKPGMVVTFAPVNVTTEVKSVEMHHEALSEALPGDNVGFNVK
NVSVKDVRRGNVAGDSKNDPPMEAAGFTAQVIILNHPGQISAGYAPVLDCHTAHIACK
FAELKEKIDRRSGKKLEDGPKFLKSGDAAIVDMVPGKPMCVESFSDYPPLGRFAVRDM
RQTVAVGVIKAVDKKAAGAGKVTKSAQKAQKAK"
misc_feature 146..349
/gene="EEF1A1"
/gene_synonym="EEF-1"
/gene_synonym="EEF1A"
/gene_synonym="eEF1A-1"
/gene_synonym="EEF1A1L14"
/gene_synonym="EF-Tu"
/gene_synonym="EF1A"
/gene_synonym="GRAF-1EF"
/gene_synonym="MGC16224"
/gene_synonym="PTI-1"
/gene_synonym="PTI1"
/note="GTP_EFTU_D2; Region: Elongation factor Tu domain 2.
Elongation factor Tu consists of three structural domains,
this is the second domain. This domain adopts a beta
barrel structure. This the second domain is involved in
binding to charged tRNA. This domain is also found in
other proteins such as elongation factor G and translation
initiation factor IF-2. This domain is structurally
related to pfam03143, and in fact has weak sequence
matches to this domain"
/db_xref="CDD:pfam03144"
misc_feature 365..664
/gene="EEF1A1"
/gene_synonym="EEF-1"
/gene_synonym="EEF1A"
/gene_synonym="eEF1A-1"
/gene_synonym="EEF1A1L14"
/gene_synonym="EF-Tu"
/gene_synonym="EF1A"
/gene_synonym="GRAF-1EF"
/gene_synonym="MGC16224"
/gene_synonym="PTI-1"
/gene_synonym="PTI1"
/note="GTP_EFTU_D3; Region: Elongation factor Tu
C-terminal domain. Elongation factor Tu consists of three
structural domains, this is the third domain. This domain
adopts a beta barrel structure. This the third domain is
involved in binding to both charged tRNA and binding to
EF-Ts pfam00889"
/db_xref="CDD:pfam03143"
BASE COUNT 305 a 222 c 261 g 283 t
ORIGIN
1 ggggggatgg aaagtcaccc gtaaggatgg caatgccagt ggaaccacgc tgcttgaggc
61 tctggactgc atcctaccac caactcgtcc aactgacaag cccttgcgcc tgcctctcca
121 ggatgtctac aaaattggtg gtattggtac tgttcctgtt ggccgagtgg agactggtgt
181 tctcaaaccc ggtatggtgg tcacctttgc tccagtcaac gttacaacgg aagtaaaatc
241 tgtcgaaatg caccatgaag ctttgagtga agctcttcct ggggacaatg tgggcttcaa
301 tgtcaagaat gtgtctgtca aggatgttcg tcgtggcaac gttgctggtg acagcaaaaa
361 tgacccacca atggaagcag ctggcttcac tgctcaggtg attatcctga accatccagg
421 ccaaataagc gccggctatg cccctgtatt ggattgccac acggctcaca ttgcatgcaa
481 gtttgctgag ctgaaggaaa agattgatcg ccgttctggt aaaaagctgg aagatggccc
541 taaattcttg aagtctggtg atgctgccat tgttgatatg gttcctggca agcccatgtg
601 tgttgagagc ttctcagact atccaccttt gggtcgcttt gctgttcgtg atatgagaca
661 gacagttgcg gtgggtgtca tcaaagcagt ggacaagaag gctgctggag ctggcaaggt
721 caccaagtct gcccagaaag ctcagaaggc taaatgaata ttatccctaa tacctgccac
781 cccactctta atcagtggtg gaagaacggt ctcagaactg tttgtttcaa ttggccattt
841 aagtttagta gtaaaagact ggttaatgat aacaatgcat cgtaaaacct tcagaaggaa
901 aggagaatgt tttgtggacc actttggttt tcttttttgc gtgtggcagt tttaagttat
961 tagtttttaa aatcagtact ttttaatgga aacaacttga ccaaaaattt gtcacagaat
1021 tttgagaccc attaaaaaag caaaaaaaaa aaaaaaaaaa aaaaaaaaaa a
//