LOCUS BC065529 3550 bp mRNA linear HUM 04-AUG-2008
DEFINITION Homo sapiens amyloid beta (A4) precursor protein, mRNA (cDNA clone
MGC:75167 IMAGE:6152423), complete cds.
ACCESSION BC065529
VERSION BC065529.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 3550)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 3550)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (26-JAN-2004) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC
cDNA Library Preparation: Life Technologies, Inc.
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: National Institutes of Health Intramural
Sequencing Center (NISC),
Gaithersburg, Maryland;
Web site: http://www.nisc.nih.gov/
Contact: nisc_mgc@nhgri.nih.gov
Akhter,N., Ayele,K., Beckstrom-Sternberg,S.M., Benjamin,B.,
Blakesley,R.W., Bouffard,G.G., Breen,K., Brinkley,C., Brooks,S.,
Dietrich,N.L., Granite,S., Guan,X., Gupta,J., Haghighi,P.,
Hansen,N., Ho,S.-L., Karlins,E., Kwong,P., Laric,P., Legaspi,R.,
Maduro,Q.L., Masiello,C., Maskeri,B., Mastrian,S.D.,McCloskey,J.C.,
McDowell,J., Pearson,R., Stantripop,S., Thomas,P.J., Touchman,J.W.,
Tsurgeon,C., Vogt,J.L., Walker,M.A., Wetherby,K.D., Wiggins,L.,
Young,A., Zhang,L.-H. and Green,E.D.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 140 Row: k Column: 21
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 41406053.
FEATURES Location/Qualifiers
source 1..3550
/db_xref="H-InvDB:HIT000261936"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:75167 IMAGE:6152423"
/tissue_type="Eye, retinoblastoma"
/clone_lib="NIH_MGC_67"
/lab_host="DH10B"
/note="Vector: pCMV-SPORT6"
gene 1..3550
/gene="APP"
/gene_synonym="AAA"
/gene_synonym="ABETA"
/gene_synonym="ABPP"
/gene_synonym="APPI"
/gene_synonym="CTFgamma"
/gene_synonym="CVAP"
/gene_synonym="PN2"
/db_xref="GeneID:351"
/db_xref="HGNC:HGNC:620"
/db_xref="MIM:104760"
CDS 132..2387
/gene="APP"
/gene_synonym="AAA"
/gene_synonym="ABETA"
/gene_synonym="ABPP"
/gene_synonym="APPI"
/gene_synonym="CTFgamma"
/gene_synonym="CVAP"
/gene_synonym="PN2"
/codon_start=1
/product="amyloid beta (A4) precursor protein"
/protein_id="AAH65529.1"
/db_xref="GeneID:351"
/db_xref="HGNC:HGNC:620"
/db_xref="MIM:104760"
/translation="MLPGLALLLLAAWTARALEVPTDGNAGLLAEPQIAMFCGRLNMH
MNVQNGKWDSDPSGTKTCIDTKEGILQYCQEVYPELQITNVVEANQPVTIQNWCKRGR
KQCKTHPHFVIPYRCLVGEFVSDALLVPDKCKFLHQERMDVCETHLHWHTVAKETCSE
KSTNLHDYGMLLPCGIDKFRGVEFVCCPLAEESDNVDSADAEEDDSDVWWGGADTDYA
DGSEDKVVEVAEEEEVAEVEEEEADDDEDDEDGDEVEEEAEEPYEEATERTTSIATTT
TTTTESVEEVVREVCSEQAETGPCRAMISRWYFDVTEGKCAPFFYGGCGGNRNNFDTE
EYCMAVCGSAIPTTAASTPDAVDKYLETPGDENEHAHFQKAKERLEAKHRERMSQVMR
EWEEAERQAKNLPKADKKAVIQHFQEKVESLEQEAANERQQLVETHMARVEAMLNDRR
RLALENYITALQAVPPRPRHVFNMLKKYVRAEQKDRQHTLKHFEHVRMVDPKKAAQIR
SQVMTHLRVIYERMNQSLSLLYNVPAVAEEIQDEVDELLQKEQNYSDDVLANMISEPR
ISYGNDALMPSLTETKTTVELLPVNGEFSLDDLQPWHSFGADSVPANTENEVEPVDAR
PAADRGLTTRPGSGLTNIKTEEISEVKMDAEFRHDSGYEVHHQKLVFFAEDVGSNKGA
IIGLMVGGVVIATVIVITLVMLKKKQYTSIHHGVVEVDAAVTPEERHLSKMQQNGYEN
PTYKFFEQMQN"
BASE COUNT 1003 a 780 c 917 g 850 t
ORIGIN
1 gtaggcgaga gcacgcggag gagcgtgcgc gggggccccg ggagacggcg gcggtggcgg
61 cgcgggcaga gcaaggacgc ggcggatccc actcgcacag cagcgcactc ggtgccccgc
121 gcagggtcgc gatgctgccc ggtttggcac tgctcctgct ggccgcctgg acggctcggg
181 cgctggaggt acccactgat ggtaatgctg gcctgctggc tgaaccccag attgccatgt
241 tctgtggcag actgaacatg cacatgaatg tccagaatgg gaagtgggat tcagatccat
301 cagggaccaa aacctgcatt gataccaagg aaggcatcct gcagtattgc caagaagtct
361 accctgaact gcagatcacc aatgtggtag aagccaacca accagtgacc atccagaact
421 ggtgcaagcg gggccgcaag cagtgcaaga cccatcccca ctttgtgatt ccctaccgct
481 gcttagttgg tgagtttgta agtgatgccc ttctcgttcc tgacaagtgc aaattcttac
541 accaggagag gatggatgtt tgcgaaactc atcttcactg gcacaccgtc gccaaagaga
601 catgcagtga gaagagtacc aacttgcatg actacggcat gttgctgccc tgcggaattg
661 acaagttccg aggggtagag tttgtgtgtt gcccactggc tgaagaaagt gacaatgtgg
721 attctgctga tgcggaggag gatgactcgg atgtctggtg gggcggagca gacacagact
781 atgcagatgg gagtgaagac aaagtagtag aagtagcaga ggaggaagaa gtggctgagg
841 tggaagaaga agaagccgat gatgacgagg acgatgagga tggtgatgag gtagaggaag
901 aggctgagga accctacgaa gaagccacag agagaaccac cagcattgcc accaccacca
961 ccaccaccac agagtctgtg gaagaggtgg ttcgagaggt gtgctctgaa caagccgaga
1021 cggggccgtg ccgagcaatg atctcccgct ggtactttga tgtgactgaa gggaagtgtg
1081 ccccattctt ttacggcgga tgtggcggca accggaacaa ctttgacaca gaagagtact
1141 gcatggccgt gtgtggcagc gccattccta caacagcagc cagtacccct gatgccgttg
1201 acaagtatct cgagacacct ggggatgaga atgaacatgc ccatttccag aaagccaaag
1261 agaggcttga ggccaagcac cgagagagaa tgtcccaggt catgagagaa tgggaagagg
1321 cagaacgtca agcaaagaac ttgcctaaag ctgataagaa ggcagttatc cagcatttcc
1381 aggagaaagt ggaatctttg gaacaggaag cagccaacga gagacagcag ctggtggaga
1441 cacacatggc cagagtggaa gccatgctca atgaccgccg ccgcctggcc ctggagaact
1501 acatcaccgc tctgcaggct gttcctcctc ggcctcgtca cgtgttcaat atgctaaaga
1561 agtatgtccg cgcagaacag aaggacagac agcacaccct aaagcatttc gagcatgtgc
1621 gcatggtgga tcccaagaaa gccgctcaga tccggtccca ggttatgaca cacctccgtg
1681 tgatttatga gcgcatgaat cagtctctct ccctgctcta caacgtgcct gcagtggccg
1741 aggagattca ggatgaagtt gatgagctgc ttcagaaaga gcaaaactat tcagatgacg
1801 tcttggccaa catgattagt gaaccaagga tcagttacgg aaacgatgct ctcatgccat
1861 ctttgaccga aacgaaaacc accgtggagc tccttcccgt gaatggagag ttcagcctgg
1921 acgatctcca gccgtggcat tcttttgggg ctgactctgt gccagccaac acagaaaacg
1981 aagttgagcc tgttgatgcc cgccctgctg ccgaccgagg actgaccact cgaccaggtt
2041 ctgggttgac aaatatcaag acggaggaga tctctgaagt gaagatggat gcagaattcc
2101 gacatgactc aggatatgaa gttcatcatc aaaaattggt gttctttgca gaagatgtgg
2161 gttcaaacaa aggtgcaatc attggactca tggtgggcgg tgttgtcata gcgacagtga
2221 tcgtcatcac cttggtgatg ctgaagaaga aacagtacac atccattcat catggtgtgg
2281 tggaggttga cgccgctgtc accccagagg agcgccacct gtccaagatg cagcagaacg
2341 gctacgaaaa tccaacctac aagttctttg agcagatgca gaactagacc cccgccacag
2401 cagcctctga agttggacag caaaaccatt gcttcactac ccatcggtgt ccatttatag
2461 aataatgtgg gaagaaacaa acccgtttta tgatttactc attatcgcct tttgacagct
2521 gtgctgtaac acaagtagat gcctgaactt gaattaatcc acacatcagt aatgtattct
2581 atctctcttt acattttggt ctctatacta cattattaat gggttttgtg tactgtaaag
2641 aatttagctg tatcaaacta gtgcatgaat agattctctc ctgattattt atcacatagc
2701 cccttagcca gttgtatatt attcttgtgg tttgtgaccc aattaagtcc tactttacat
2761 atgctttaag aatcgatggg ggatgcttca tgtgaacgtg ggagttcagc tgcttctctt
2821 gcctaagtat tcctttcctg atcactatgc attttaaagt taaacatttt taagtatttc
2881 agatgcttta gagagatttt ttttccatga ctgcatttta ctgtacagat tgctgcttct
2941 gctatatttg tgatatagga attaagagga tacacacgtt tgtttcttcg tgcctgtttt
3001 atgtgcacac attaggcatt gagacttcaa gcttttcttt ttttgtccac gtatctttgg
3061 gtctttgata aagaaaagaa tccctgttca ttgtaagcac ttttacgggg cgggtgggga
3121 ggggtgctct gctggtcttc aattaccaag aattctccaa aacaattttc tgcaggatga
3181 ttgtacagaa tcattgctta tgacatgatc gctttctaca ctgtattaca taaataaatt
3241 aaataaaata accccgggca agacttttct ttgaaggatg actacagaca ttaaataatc
3301 gaagtaattt tgggtgggga gaagaggcag attcaatttt ctttaaccag tctgaagttt
3361 catttatgat acaaaagaag atgaaaatgg aagtggcaat ataaggggat gaggaaggca
3421 tgcctggaca aacccttctt ttaagatgtg tcttcaattt gtataaaatg gtgttttcat
3481 gtaaataaat acattcttgg aggaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
3541 aaaaaaaaaa
//