LOCUS BC065525 3777 bp mRNA linear HUM 27-JAN-2004
DEFINITION Homo sapiens adducin 2 (beta), mRNA (cDNA clone MGC:74968
IMAGE:6142886), complete cds.
ACCESSION BC065525
VERSION BC065525.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 3777)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 3777)
AUTHORS Strausberg,R.
TITLE Direct Submission
JOURNAL Submitted (26-JAN-2004) National Institutes of Health, Mammalian
Gene Collection (MGC), Cancer Genomics Office, National Cancer
Institute, 31 Center Drive, Room 11A03, Bethesda, MD 20892-2590,
USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC
cDNA Library Preparation: Life Technologies, Inc.
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: National Institutes of Health Intramural
Sequencing Center (NISC),
Gaithersburg, Maryland;
Web site: http://www.nisc.nih.gov/
Contact: nisc_mgc@nhgri.nih.gov
Akhter,N., Ayele,K., Beckstrom-Sternberg,S.M., Benjamin,B.,
Blakesley,R.W., Bouffard,G.G., Breen,K., Brinkley,C., Brooks,S.,
Dietrich,N.L., Granite,S., Guan,X., Gupta,J., Haghighi,P.,
Hansen,N., Ho,S.-L., Karlins,E., Kwong,P., Laric,P., Legaspi,R.,
Maduro,Q.L., Masiello,C., Maskeri,B., Mastrian,S.D.,McCloskey,J.C.,
McDowell,J., Pearson,R., Stantripop,S., Thomas,P.J., Touchman,J.W.,
Tsurgeon,C., Vogt,J.L., Walker,M.A., Wetherby,K.D., Wiggins,L.,
Young,A., Zhang,L.-H. and Green,E.D.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 140 Row: i Column: 19
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 9257177.
FEATURES Location/Qualifiers
source 1..3777
/db_xref="H-InvDB:HIT000261932"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:74968 IMAGE:6142886"
/tissue_type="Eye, retinoblastoma"
/clone_lib="NIH_MGC_67"
/lab_host="DH10B"
/note="Vector: pCMV-SPORT6"
gene 1..3777
/gene="ADD2"
/gene_synonym="ADDB"
/db_xref="GeneID:119"
/db_xref="MIM:102681"
CDS 266..2446
/gene="ADD2"
/gene_synonym="ADDB"
/codon_start=1
/product="ADD2 protein"
/protein_id="AAH65525.1"
/db_xref="GeneID:119"
/db_xref="MIM:102681"
/translation="MSEETVPEAASPPPPQGQPYFDRFSEDDPEYMRLRNRAADLRQD
FNLMEQKKRVTMILQSPSFREELEGLIQEQMKKGNNSSNIWALRQIADFMASTSHAVF
PTSSMNVSMMTPINDLHTADSLNLAKGERLMRCKISSVYRLLDLYGWAQLSDTYVTLR
VSKEQDHFLISPKGVSCSEVTASSLIKVNILGEVVEKGSSCFPVDTTGFCLHSAIYAA
RPDVRCIIHLHTPATAAVSAMKWGLLPVSHNALLVGDMAYYDFNGEMEQEADRINLQK
CLGPTCKILVLRNHGVVALGDTVEEAFYKIFHLQAACEIQVSALSSAGGVENLILLEQ
EKHRPHEVGSVQWAGSTFGPMQKSRLGEHEFEALMRMLDNLGYRTGYTYRHPFVQEKT
KHKSEVEIPATVTAFVFEEDGAPVPALRQHAQKQQKEKTRWLNTPNAYLRVNVADEVQ
RSMGSPRPKTTWMKADEVEKSSSGMPIRIENPNQFVPLYTDPQEVLEMRNKIREQNRQ
DVKSAGPQSQLLASVIAEKSRSPSTESQLMSKGDEDTKDDSEETVPNPFSQLTDQELE
EYKKEVERKKLELDGEKETAPEEPGSPAKSAPASPVQSPAKEAETKSPLVSPSKSLEE
GTKKTETSKAATTEPETTQPEGVVVNGREEEQTAEEILSKGLSQMTTSADTDVDTSKD
KTESVTSGPMSPEGSPSKSPSKKKKKFRTPSFLKKSKKKEKVES"
misc_feature 653..1285
/gene="ADD2"
/gene_synonym="ADDB"
/note="Aldolase_II; Region: Class II Aldolase and Adducin
head (N-terminal) domain. Aldolases are ubiquitous enzymes
catalyzing central steps of carbohydrate metabolism. Based
on enzymatic mechanisms, this superfamily has been divided
into two distinct classes (Class I and II). Class II
enzymes are further divided into two sub-classes A and B.
This family includes class II A aldolases and adducins
which has not been ascribed any enzymatic function.
Members of this class are primarily bacterial and
eukaryotic in origin and include L-fuculose-1-phosphate,
L-rhamnulose-1-phosphate aldolases and
L-ribulose-5-phosphate 4-epimerases. They all share the
ability to promote carbon-carbon bond cleavage and
stabilize enolate intermediates using divalent cations"
/db_xref="CDD:cd00398"
BASE COUNT 982 a 1023 c 1012 g 760 t
ORIGIN
1 aaaggtttct ggtgatgatg tagcatccga ggctcttagg aaatcggggc tgcaagcact
61 ggcccctgct tggggatggt gggaccctcg cccggagcgt aacgcctgac agtttccacc
121 tggatgtttg aggttgtgta gatgtggccg gcacccttga gagtggagct agggggtgca
181 gactgagcag tgaacagaag gagccttgga cagggctggg ccagcctccc gagttccagg
241 agcgaattgc aaacccaccg ggaaaatgag cgaagagacg gtccccgagg ctgcctcgcc
301 gccgcccccg caggggcagc cttactttga ccgcttctca gaggacgacc ccgagtacat
361 gcgccttcgc aaccgggcgg cggacctgcg gcaggacttc aacctgatgg agcagaagaa
421 gcgcgtcacc atgatcctgc agagtccctc tttcagggag gagctggaag gcctcatcca
481 ggagcagatg aagaagggga acaactcctc caacatctgg gccctgcgac agatcgcgga
541 cttcatggcc agcacctccc acgcagtctt cccgacatct tccatgaatg tctccatgat
601 gacgcctatc aatgacctcc acacagctga ctccctgaac ctggccaaag gggagcggct
661 catgcggtgc aagatcagca gtgtctaccg actcctggac ctctatggct gggcccagct
721 gagtgacacc tatgtcacgt tgagagtcag caaggagcag gaccacttcc tgatcagccc
781 taagggagtt tcttgcagtg aagtcacagc gtccagcctg atcaaggtga acattctggg
841 agaggtggtg gagaagggca gcagctgctt cccagtggac accacaggct tctgtctgca
901 ctcggccatc tatgcagcga ggcccgacgt gcgctgcatc atccacctgc acacaccggc
961 cacagcagcg gtgtcggcca tgaagtgggg cctcctgcct gtctcccaca atgccctgct
1021 ggtgggggac atggcctatt atgacttcaa tggggaaatg gagcaggaag ccgatcggat
1081 caacctgcag aagtgccttg gacccacctg caagatcctg gtgctaagaa accatggagt
1141 ggttgctctg ggtgacacgg tagaggaggc attttacaag atcttccacc tgcaggctgc
1201 atgtgagata caggtgtcgg ctctgtccag tgccggggga gtggagaacc tcatcctcct
1261 ggagcaggag aagcaccggc cccatgaggt gggctccgtg cagtgggccg ggagcacctt
1321 tgggcctatg cagaagagtc ggctggggga gcatgagttt gaggccctca tgaggatgct
1381 ggacaacctg ggctacagaa caggttacac gtatcgccac ccctttgttc aagagaaaac
1441 caaacacaaa agtgaggtgg agattccagc cacggtcaca gccttcgtgt ttgaggagga
1501 cggtgccccg gtgcccgccc tgcgacagca tgcccagaag cagcagaagg agaagacccg
1561 ctggctcaat acgcccaacg cctacctgcg ggtcaatgtg gccgatgagg tccagaggag
1621 catgggcagc ccccgaccca agaccacgtg gatgaaggct gacgaggtgg agaaatccag
1681 cagtggcatg ccgattcgca tcgaaaaccc aaaccaattt gtgcctctct atactgaccc
1741 ccaggaagta ctggagatga ggaacaagat tcgagaacaa aaccgacaag atgtgaagtc
1801 agcggggcct cagtcccagc tcctggcgag cgtcattgcc gagaagagcc gaagcccgtc
1861 tacagagagc cagctgatgt ccaagggaga cgaggatacc aaagacgatt cagaggagac
1921 ggtgcccaac cccttcagcc aactcactga ccaggagttg gaggagtaca agaaagaggt
1981 ggagaggaag aaactagaac ttgatggaga gaaagaaact gccccagaag agcctggctc
2041 acctgcaaag tctgcacctg cttctccagt gcagagccca gcgaaggagg cagagacaaa
2101 gagcccttta gtctctcctt ccaagtcttt agaggaaggt actaagaaga cagaaacaag
2161 caaagccgcc accacagagc ccgaaacaac ccagccggaa ggggtggtgg tcaacgggag
2221 ggaggaggag cagacggcag aggaaatcct cagcaaaggc ctgagccaga tgaccaccag
2281 tgctgacacg gatgttgata cctctaagga caaaaccgag tcggtcacca gcggccccat
2341 gtccccagag ggctcacctt ccaagtctcc ctcaaagaag aaaaagaaat tccgaacccc
2401 ctccttcctg aaaaagagca aaaagaagga gaaagtggag tcctgattca tgacaccctt
2461 gggctccctc ctgcctcctc tctctcctcc ccttcccttc tcccatctct gtccctgcaa
2521 gcacagggct aaggagggat agagtaggac cctggaccac attcggaagg ggaacttaga
2581 gatcacccga ccaacccttc gttttacagt tgcccaagag aaatcaggtg acttgcccaa
2641 ggtcacacag ctagttagcg gcagagcctg cactcgaatt caggtctcct gacttccagt
2701 ccagtgctcc ttctactaca caacactgcc tagttgtggg ctgcctttgt ttggatgctg
2761 tccaccaatc tgagcctagg gcaagaaggc cagaaatggg ccgtgagctc tcacaggctc
2821 agactaaatc agaggtcaag gcttcccctg agtaaggtcc atttcttccc aggaatccaa
2881 tctcctgtgg atggagctat ctctacattt aaaaatctct tctcttttcc actttgggtc
2941 cctgccctgc tgctcaaagt gactagccaa attgacccct ccaacagaaa gtaatctttg
3001 ttcccaaggg ctgatggctt agcttgtact accccaaaca ttaaccctga gctttcttca
3061 tggaacctct tgaatgatgg atggaagagc tataagaggt ggtaggcata ggggcaagcc
3121 atgtaagctg aggattgggg atggtttcat caacataaga ggccaggaac ttgacccctt
3181 tgaattgtgc atctcaggca cttcaaaact aaaaccaaat ttagcatagg aaaaagttgt
3241 ttaatgctca gggcagaaat ttggggaagt tgaaatcctc tgttggcttt gggttgtata
3301 aggaggatca aaacaacaga ggaaatgctg actttctagc tttgcatgac acctggagca
3361 atgcactgta cctgcctcac tcctgtccag tggtcaggtt tcccctgacc ttccctcacc
3421 cccagaaaca cttgcttaca gaccgaaact ggcatcttac tcttggcacc ttgacttgca
3481 ccctctgagg ttccaactca gtcattcttt gtccagcaga ggagaatcag aaatgagccc
3541 ttcaggatta atcctcttgc accagctctc agagaaatgc tgggtatccc tgtccttgtc
3601 cctatctgtc catcctgggg cctggtaatg gccacagtta ttgttttaaa tgccaacact
3661 gtcttctcat gttcttccgt ggggcattga ttaatgagca tttgttggct cctaaaaatt
3721 agacaatcca ttctcttgaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaa
//