LOCUS BC065282 2598 bp mRNA linear HUM 21-JUL-2006
DEFINITION Homo sapiens phosphatidylinositol glycan anchor biosynthesis, class
O, mRNA (cDNA clone MGC:74535 IMAGE:6172460), complete cds.
ACCESSION BC065282
VERSION BC065282.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2598)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 2598)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (12-JAN-2004) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC
cDNA Library Preparation: Life Technologies, Inc.
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: National Institutes of Health Intramural
Sequencing Center (NISC),
Gaithersburg, Maryland;
Web site: http://www.nisc.nih.gov/
Contact: nisc_mgc@nhgri.nih.gov
Akhter,N., Ayele,K., Beckstrom-Sternberg,S.M., Benjamin,B.,
Blakesley,R.W., Bouffard,G.G., Breen,K., Brinkley,C., Brooks,S.,
Dietrich,N.L., Granite,S., Guan,X., Gupta,J., Haghighi,P.,
Hansen,N., Ho,S.-L., Karlins,E., Kwong,P., Laric,P., Legaspi,R.,
Maduro,Q.L., Masiello,C., Maskeri,B., Mastrian,S.D.,McCloskey,J.C.,
McDowell,J., Pearson,R., Stantripop,S., Thomas,P.J., Touchman,J.W.,
Tsurgeon,C., Vogt,J.L., Walker,M.A., Wetherby,K.D., Wiggins,L.,
Young,A., Zhang,L.-H. and Green,E.D.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 140 Row: p Column: 7
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 38045916.
FEATURES Location/Qualifiers
source 1..2598
/db_xref="H-InvDB:HIT000261845"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:74535 IMAGE:6172460"
/tissue_type="Eye, retinoblastoma"
/clone_lib="NIH_MGC_67"
/lab_host="DH10B"
/note="Vector: pCMV-SPORT6"
gene 1..2598
/gene="PIGO"
/gene_synonym="DKFZp434M222"
/gene_synonym="FLJ00135"
/gene_synonym="MGC20536"
/gene_synonym="MGC3079"
/gene_synonym="RP11-182N22.4"
/db_xref="GeneID:84720"
/db_xref="HGNC:HGNC:23215"
CDS 166..2184
/gene="PIGO"
/gene_synonym="DKFZp434M222"
/gene_synonym="FLJ00135"
/gene_synonym="MGC20536"
/gene_synonym="MGC3079"
/gene_synonym="RP11-182N22.4"
/codon_start=1
/product="phosphatidylinositol glycan anchor biosynthesis,
class O"
/protein_id="AAH65282.1"
/db_xref="GeneID:84720"
/db_xref="HGNC:HGNC:23215"
/translation="MQKASVLLFLAWVCFLFYAGIALFTSGFLLTRLELTNHSSCQEP
PGPGSLPWGSQGKPGACWMASRFSRVVLVLIDALRFDFAQPQHSHVPREPPVSLPFLG
KLSSLQRILEIQPHHARLYRSQVDPPTTTMQRLKALTTGSLPTFIDAGSNFASHAIVE
DNLIKQLTSAGRRVVFMGDDTWKDLFPGAFSKAFFFPSFNVRDLDTVDNGILEHLYPT
MDSGEWDVLIAHFLGVDHCGHKHGPHHPEMAKKLSQMDQVIQGLVERLENDTLLVVAG
DHGMTTNGDHGGDSELEVSAALFLYSPTAVFPSTPPEEPEVIPQVSLVPTLALLLGLP
IPFGNIGEVMAELFSGGEDSQPHSSALAQASALHLNAQQVSRFLHTYSAATQDLQAKE
LHQLQNLFSKASADYQWLLQSPKGAEATLPTVIAELQQFLRGARAMCIESWARFSLSF
LLLHLLAAGIPVTTPGPFTVPWQAVSAWALMATQTFYSTGHQPVFPAIHWHAAFVGFP
EGHGSCTWLPALLVGANTFASHLLFAVGCPLLLLWPFLCESQGLRKRQQPPGNEADAR
VRPEEEEEPLMEMRLRDAPQHFYAALLQLGLKYLFILGIQILACALAASILRRHLMVW
KVFAPKFIFEAVGFIVSSVGLLLGIALVMRVDGAVSSWFRQLFLAQQR"
BASE COUNT 537 a 755 c 685 g 621 t
ORIGIN
1 cctgcggctt cgatcccttt ccactgggta ttgagattgg cgaggggaga ggcgcagaaa
61 cggcgtactc tagtgcagtg gttcaatctc ggctcactgc aacctccgcc tccccgattc
121 aagcgattct cctgcgtcag cctccccagt agctgagact acaggatgca gaaagcctca
181 gtgttgctct tcctggcctg ggtctgcttc ctcttctacg ctggcattgc cctcttcacc
241 agtggcttcc tgctcacccg tttggagctc accaaccata gcagctgcca agagccccca
301 ggccctgggt ccctgccatg ggggagccaa gggaaacctg gggcctgctg gatggcttcc
361 cgattttcgc gggttgtgtt ggtgctgata gatgctctgc gatttgactt cgcccagccc
421 cagcattcac acgtgcctag agagcctcct gtctccctac ccttcctggg caaactaagc
481 tccttgcaga ggatcctgga gattcagccc caccatgccc ggctctaccg atctcaggtt
541 gaccctccta ccaccaccat gcagcgcctc aaggccctca ccactggctc actgcctacc
601 tttattgatg ctggtagtaa cttcgccagc cacgccatag tggaagacaa tctcattaag
661 cagctcacca gtgcaggaag gcgtgtagtc ttcatgggag atgatacctg gaaagacctt
721 ttccctggtg ctttctccaa agctttcttc ttcccatcct tcaatgtcag agacctagac
781 acagtggaca atggcatcct ggaacacctc taccccacca tggacagtgg tgaatgggac
841 gtgctgattg ctcacttcct gggtgtggac cactgtggcc acaagcatgg ccctcaccac
901 cctgaaatgg ccaagaaact tagccagatg gaccaggtga tccagggact tgtggagcgt
961 ctggagaatg acacactgct ggtagtggct ggggaccatg ggatgaccac aaatggagac
1021 catggagggg acagtgagct ggaggtctca gctgctctct ttctgtatag ccccacagca
1081 gtcttcccca gcaccccacc agaggagcca gaggtgattc ctcaagttag ccttgtgccc
1141 acgctggccc tgctgctggg cctgcccatc ccatttggga atatcgggga agtgatggct
1201 gagctattct cagggggtga ggactcccag ccccactcct ctgctttagc ccaagcctca
1261 gctctccatc tcaatgctca gcaggtgtcc cgatttcttc atacctactc agctgctact
1321 caggaccttc aagctaagga gcttcatcag ctgcagaacc tcttctccaa ggcctctgct
1381 gactaccagt ggcttctcca gagccccaag ggggctgagg cgacactgcc gactgtgatt
1441 gctgagctgc agcagttcct gcggggagct cgggccatgt gcatcgagtc ttgggctcgt
1501 ttctctctga gcttccttct cctacatctg cttgctgctg ggatacccgt caccacccct
1561 ggtcctttta ctgtgccatg gcaggcagtc tcggcttggg ccctcatggc cacacagacc
1621 ttctactcca caggccacca gcctgtcttt ccagccatcc attggcatgc agccttcgtg
1681 ggattcccag agggtcatgg ctcctgtact tggctgcctg ctttgctagt gggagccaac
1741 acctttgcct cccacctcct ctttgcagta ggttgcccac tgctcctgct ctggcctttc
1801 ctgtgtgaga gtcaagggct gcggaagaga cagcagcccc cagggaatga agctgatgcc
1861 agagtcagac ccgaggagga agaggagcca ctgatggaga tgcggctccg ggatgcgcct
1921 cagcacttct atgcagcact gctgcagctg ggcctcaagt acctctttat ccttggtatt
1981 cagattctgg cctgtgcctt ggcagcctcc atccttcgca ggcatctcat ggtctggaaa
2041 gtgtttgccc ctaagttcat atttgaggct gtgggcttca ttgtgagcag cgtgggactt
2101 ctcctgggca tagctttggt gatgagagtg gatggtgctg tgagctcctg gttcaggcag
2161 ctatttctgg cccagcagag gtagcctagt ctgtgattac tggcacttgg ctacagagag
2221 tgctggagaa cagtgtagcc tggcctgtac aggtactgga tgatctgcaa gacaggctca
2281 gccatactct tactatcatg cagccagggg ccgctgacat ctaggacttc attattctat
2341 aattcaggac cacagtggag tatgatccct aactcctgat ttggatgcat ctgagggaca
2401 aggggggcgg tctccgaagt ggaataaaat aggccgggcg tggtgacttg cacctataat
2461 cccagcactt tgggaggcag aggtgggagg attgcttggt cccaggagtt caagaccagc
2521 ctgtggaaca taacaagacc ccgtctctac tatttaaaaa aaagtgtaat aaaatgataa
2581 tataaaaaaa aaaaaaaa
//