LOCUS BC065276 4015 bp mRNA linear HUM 28-JUL-2006
DEFINITION Homo sapiens eukaryotic translation initiation factor 4 gamma, 2,
mRNA (cDNA clone MGC:75160 IMAGE:6164568), complete cds.
ACCESSION BC065276
VERSION BC065276.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 4015)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 4015)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (12-JAN-2004) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC/DCTD/DTP
cDNA Library Preparation: Life Technologies, Inc.
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: National Institutes of Health Intramural
Sequencing Center (NISC),
Gaithersburg, Maryland;
Web site: http://www.nisc.nih.gov/
Contact: nisc_mgc@nhgri.nih.gov
Akhter,N., Ayele,K., Beckstrom-Sternberg,S.M., Benjamin,B.,
Blakesley,R.W., Bouffard,G.G., Breen,K., Brinkley,C., Brooks,S.,
Dietrich,N.L., Granite,S., Guan,X., Gupta,J., Haghighi,P.,
Hansen,N., Ho,S.-L., Karlins,E., Kwong,P., Laric,P., Legaspi,R.,
Maduro,Q.L., Masiello,C., Maskeri,B., Mastrian,S.D.,McCloskey,J.C.,
McDowell,J., Pearson,R., Stantripop,S., Thomas,P.J., Touchman,J.W.,
Tsurgeon,C., Vogt,J.L., Walker,M.A., Wetherby,K.D., Wiggins,L.,
Young,A., Zhang,L.-H. and Green,E.D.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 140 Row: n Column: 17
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 4503538.
FEATURES Location/Qualifiers
source 1..4015
/db_xref="H-InvDB:HIT000261839"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:75160 IMAGE:6164568"
/tissue_type="Skin, melanotic melanoma."
/clone_lib="NIH_MGC_72"
/lab_host="DH10B"
/note="Vector: pCMV-SPORT6"
gene 1..4015
/gene="EIF4G2"
/gene_synonym="AAG1"
/gene_synonym="DAP5"
/gene_synonym="NAT1"
/gene_synonym="p97"
/db_xref="GeneID:1982"
/db_xref="HGNC:HGNC:3297"
/db_xref="MIM:602325"
CDS 1130..3235
/gene="EIF4G2"
/gene_synonym="AAG1"
/gene_synonym="DAP5"
/gene_synonym="NAT1"
/gene_synonym="p97"
/codon_start=1
/product="EIF4G2 protein"
/protein_id="AAH65276.1"
/db_xref="GeneID:1982"
/db_xref="HGNC:HGNC:3297"
/db_xref="MIM:602325"
/translation="MLGNIKFIGELGKLDLIHESILHKCIKTLLEKKKRVQLKDMGED
LECLCQIMRTVGPRLDHERAKSLMDQYFARMCSLMLSKELPARIRFLLQDTVELREHH
WVPRKAFLDNGPKTINQIRQDAVKDLGVFIPAPMAQGMRSDFFLEGPFMPPRMKMDRD
PLGGLADMFGQMPGSGIGTGPGVIQDRFSPTMGRHRSNQLFNGHGGHIMPPTQSQFGE
MGGKFMKSQGLSQLYHNQSQGLLSQLQGQSKDMPPRFSKKGQLNADEISLRPAQSFLM
NKNQVPKLQPQITMIPPSAQPPRTQTPPLGQTPQLGLKTNPPLIQEKPAKTSKKPPPS
KEELLKLTETVVTEYLNSGNANEAVNGVREMRAPKHFLPEMLSKVIILSLDRSDEDKE
KASSLISLLKQEGIATSDNFMQAFLNVLDQCPKLEVDIPLVKSYLAQFAARAIISELV
SISELAQPLESGTHFPLFLLCLQQLAKLQDREWLTELFQQSKVNMQKMLPEIDQNKDR
MLEILEGKGLSFLFPLLKLEKELLKQIKLDPSPQTIYKWIKDNISPKLHVDKGFVNIL
MTSFLQYISSEVNPPSDETDSSSAPSKEQLEQEKQLLLSFKPVMQKFLHDHVDLQVSA
LYALQVHCYNSNFPKGMLLRFFVHFYDMEIIEEEAFLAWKEDITQEFPGKGKALFQVN
QWLTWLETAEEEESEEEAD"
BASE COUNT 1213 a 848 c 876 g 1078 t
ORIGIN
1 gggaggaatg ctccgctctt ttgttcccag gaagggaaaa acggggcgga ggttcctgct
61 gccatgttgt ggccctggtc ttccgcctgc aagtgctagt cctgtacacc cggccccctc
121 tcgcgcgtct ttccacgagc tctccacggg gaggaggcga aagggtggac aggggaaaac
181 tgctgaagag ctttcggaga gacctcatct agtcgctggc ctcgtcaagg cagtaaaaat
241 tcctagggca gacattttcc acgtgatgtt gctcgtgtct gtagttagac ttttctattg
301 atctcagtgc tcagttttaa agatagtaga atgtggcttt ttcaaaagat tcgttctgag
361 ccgtttaaaa tgctcttgtg ttctacgaag tctctttgca tccttgaaca ccggggaatt
421 tccaggtatt tcatttctcc tgtcccctcc cctccccacc ccatctatta atattattct
481 tttgaagatt cttcgttgtc aagccgccaa agtggagagt gcgattgcag aagggggtgc
541 ttctcgtttc agtgcttctt cgggcggagg aggaagtagg ggtgcacctc agcactatcc
601 caagactgct ggcaacagcg agttcctggg gaaaacccca gggcaaaacg ctcagaaatg
661 gattcctgca cgaagcacta gacgagatga caactccgca gcaaacaact ccgcaaacga
721 aaaagaacga catgatgcaa tcttcaggaa agtaagaggc atactaaata agcttactcc
781 tgaaaagttt gacaagctat gccttgagct cctcaatgtg ggtgtagagt ctaaactcat
841 ccttaaaggg gtcatactgc tgattgtgga caaagcccta gaagagccaa agtatagctc
901 actgtatgct cagctatgtc tgcgattggc agaagatgca ccaaactttg atggcccagc
961 agcagagggt caaccaggac agaagcaaag caccacattc agacgcctcc taatttccaa
1021 attacaagat gaatttgaaa accgaactag aaatgttgat gtctatgata agcgtgaaaa
1081 tcccctcctc cccgaggagg aggaacagag agccattgct aagatcaaga tgttgggaaa
1141 catcaaattc attggagagc ttggcaagct tgatcttatt cacgaatcta tccttcataa
1201 gtgcatcaaa acacttttgg aaaagaagaa gagagtccaa ctcaaagata tgggagagga
1261 tttggagtgc ctctgtcaga taatgaggac agtgggacct agattagacc atgaacgagc
1321 caagtcctta atggatcagt actttgcccg aatgtgctcc ttgatgttaa gtaaggaatt
1381 gccagcaagg attcgtttcc tgctgcagga taccgtagag ttgcgagaac accattgggt
1441 tcctcgcaag gcttttcttg acaatggacc aaagacgatc aatcaaattc gtcaagatgc
1501 agtaaaagat ctaggggtgt ttattcctgc tcctatggct caagggatga gaagtgactt
1561 ctttctggag ggaccgttca tgccacccag gatgaaaatg gatagggacc cacttggagg
1621 acttgctgat atgtttggac aaatgccagg tagcggaatt ggtactggtc caggagttat
1681 ccaggataga ttttcaccca ccatgggacg tcatcgttca aatcaactct tcaatggcca
1741 tgggggacac atcatgcctc ccacacaatc gcagtttgga gagatgggag gcaagtttat
1801 gaaaagccag gggctaagcc agctctacca taaccagagt cagggactct tatcccagct
1861 gcaaggacag tcgaaggata tgccacctcg gttttctaag aaaggacagc ttaatgcaga
1921 tgagattagc ctgaggcctg ctcagtcgtt cctaatgaat aaaaatcaag tgccaaagct
1981 tcagccccag ataactatga ttcctcctag tgcacaacca ccacgcactc aaacaccacc
2041 tctgggacag acacctcagc ttggtctcaa aactaatcca ccacttatcc aggaaaagcc
2101 tgccaagacc agcaaaaagc caccaccgtc aaaggaagaa ctccttaaac taactgaaac
2161 tgttgtgact gaatatctaa atagtggaaa tgcaaatgag gctgtcaatg gtgtaagaga
2221 aatgagggct cctaaacact ttcttcctga gatgttaagc aaagtaatca tcctgtcact
2281 agatagaagc gatgaagata aagaaaaagc aagttctttg atcagtttac tcaaacagga
2341 agggatagcc acaagtgaca acttcatgca ggctttcctg aatgtattgg accagtgtcc
2401 caaactggag gttgacatcc ctttggtgaa atcctattta gcacagtttg cagctcgtgc
2461 catcatttca gagctggtga gcatttcaga actagctcaa ccactagaaa gtggcaccca
2521 ttttcctctc tttctacttt gtcttcagca gttagctaaa ttacaagatc gagaatggtt
2581 aacagaactt tttcaacaaa gcaaggtcaa tatgcagaaa atgctcccag aaattgatca
2641 gaataaggac cgcatgttgg agattttgga aggaaaggga ctgagtttct tattcccact
2701 cctcaaattg gagaaggaac tgttgaagca aataaagttg gatccatccc ctcaaaccat
2761 atataaatgg attaaagata acatctctcc caaacttcat gtagataaag gatttgtgaa
2821 catcttaatg actagcttct tacagtacat ttctagtgaa gtaaaccccc ccagcgatga
2881 aacagattca tcctctgctc cttccaaaga acagttagag caggaaaaac aactactact
2941 atctttcaag ccagtaatgc agaaatttct tcatgatcac gttgatctac aagtcagtgc
3001 cctgtatgct ctccaggtgc actgctataa cagcaacttc ccaaaaggca tgttacttcg
3061 cttttttgtg cacttctatg acatggaaat tattgaagaa gaagctttct tggcttggaa
3121 agaagatata acccaagagt ttccgggaaa aggcaaggct ttgttccagg tgaatcagtg
3181 gctaacctgg ttagaaactg ctgaagaaga agaatcagag gaagaagctg actaaagaac
3241 cagccaaagc cttaaattgt gcaaaacata ctgttgctat gatgtaactg catttgacct
3301 aaccactgcg aaaattcatt ccgctgtaat gttttcacaa tatttaaagc agaagcacgt
3361 cagttaggat ttccttctgc ataaggtttt tttgtagtgt aatgtcttaa tcatagtcta
3421 ccatcaaata ttttaggagt atctttaatg tttagatagt atattagcag catgcaataa
3481 ttacatcata agttctcaag cagaggcagt ctattgcaag gaccttcttt gctgccagtt
3541 atcataggct gttttaagtt agaaaactga atagcaacac tgaatactgt agaaatgcac
3601 tttgctcagt aatacttgag ttgttgcaat atttgattat ccatttggtt gttacagaaa
3661 aattcttaac tgtaattgat ggttgttgcc gtaatagtat attgcctgta tttctacctc
3721 tagtaatggg ctttatgtgc tagattttaa tatccttgag cctgggcaag tgcacaagtc
3781 tttttaaaag aaacatggtt tacttgcaca aaactgatca gttttgagag atcgttaatg
3841 cccttgaagt ggtttttgtg ggtgtgaaac aaatggtgag aatttgaatt ggtccctcct
3901 attatagtat tgaaattaag tctacttaat ttatcaagtc atgttcatgc cctgatttta
3961 tatacttgta tctatcaata aacattgtga tacttgatgt aaaaaaaaaa aaaaa
//