LOCUS BC065266 3778 bp mRNA linear HUM 26-APR-2004
DEFINITION Homo sapiens glucosidase, alpha; neutral AB, mRNA (cDNA clone
MGC:70558 IMAGE:6155944), complete cds.
ACCESSION BC065266
VERSION BC065266.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 3778)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 3778)
AUTHORS Strausberg,R.
TITLE Direct Submission
JOURNAL Submitted (12-JAN-2004) National Institutes of Health, Mammalian
Gene Collection (MGC), Cancer Genomics Office, National Cancer
Institute, 31 Center Drive, Room 11A03, Bethesda, MD 20892-2590,
USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC
cDNA Library Preparation: Life Technologies, Inc.
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: National Institutes of Health Intramural
Sequencing Center (NISC),
Gaithersburg, Maryland;
Web site: http://www.nisc.nih.gov/
Contact: nisc_mgc@nhgri.nih.gov
Akhter,N., Ayele,K., Beckstrom-Sternberg,S.M., Benjamin,B.,
Blakesley,R.W., Bouffard,G.G., Breen,K., Brinkley,C., Brooks,S.,
Dietrich,N.L., Granite,S., Guan,X., Gupta,J., Haghighi,P.,
Hansen,N., Ho,S.-L., Karlins,E., Kwong,P., Laric,P., Legaspi,R.,
Maduro,Q.L., Masiello,C., Maskeri,B., Mastrian,S.D.,McCloskey,J.C.,
McDowell,J., Pearson,R., Stantripop,S., Thomas,P.J., Touchman,J.W.,
Tsurgeon,C., Vogt,J.L., Walker,M.A., Wetherby,K.D., Wiggins,L.,
Young,A., Zhang,L.-H. and Green,E.D.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 140 Row: l Column: 13
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 21361455.
FEATURES Location/Qualifiers
source 1..3778
/db_xref="H-InvDB:HIT000261829"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:70558 IMAGE:6155944"
/tissue_type="Uterus, leiomyosarcoma"
/clone_lib="NIH_MGC_71"
/lab_host="DH10B"
/note="Vector: pCMV-SPORT6"
gene 1..3778
/gene="GANAB"
/gene_synonym="G2AN"
/gene_synonym="GluII"
/gene_synonym="KIAA0088"
/db_xref="GeneID:23193"
/db_xref="MIM:104160"
CDS 186..2729
/gene="GANAB"
/gene_synonym="G2AN"
/gene_synonym="GluII"
/gene_synonym="KIAA0088"
/codon_start=1
/product="GANAB protein"
/protein_id="AAH65266.1"
/db_xref="GeneID:23193"
/db_xref="MIM:104160"
/translation="MTRFRIDELEPRRPRYRVPDVLVADPPIARLSVSGRDENSVELT
MAEGPYKIILTARPFRLDLLEDRSLLLSVNARGLLEFEHQRAPRVSQGSKDPAEGDGA
QPEETPRDGDKPEETQGKAEKDEPGAWEETFKTHSDSKPYGPMSVGLDFSLPGMEHVY
GIPEHADNLRLKVTEGGEPYRLYNLDVFQYELYNPMALYGSVPVLLAHNPHRDLGIFW
LNAAETWVDISSNTAGKTLFGKMMDYLQGSGETPQTDVRWMSETGIIDVFLLLGPSIS
DVFRQYASLTGTQALPPLFSLGYHQSCWNYRDEADVLEVDQGFDDHNLPCDVIWLDIE
HADGKRYFTWDPSRFPQPRTMLERLASKRWKLVAIVDPHIKVDSGYRVHEELRNLGLY
VKTRDGSDYEGWCWPGSAGYPDFTNPTMRAWWANMFSYDNYEGSAPNLFVWNDMNEPS
VFNGPEVTMLKDAQHYGGWEHRDVHNIYGLYVHMATADGLRQRSGGMERPFVLARAFF
AGSQRFGAVWTGDNTAEWDHLKISIPMCLSLGLVGLSFCGADVGGFFKNPEPELLVRW
YQMGAYQPFFRAHAHLDTGRREPWLLPSQHNDIIRDALGQRYSLLPFWYTLLYQAHRE
GIPVMRPLWVQYPQDVTTFNIDDQYLLGDALLVHPVSDSGAHGVQVYLPGQGEVWYDI
QSYQKHHGPQTLYLPVTLSSIPVFQRGGTIVPRWMRVRRSSECMKDDPITLFVALSPQ
GTAQGELFLDDGHTFNYQTRQEFLLRRFSFSGNTLVSSSADPEGHFETPIWIERVVII
GAGKPAAVVLQTKGSPESRLSFQHDPETSVLVLRKPGINVASDWSIHLR"
misc_feature 549..2609
/gene="GANAB"
/gene_synonym="G2AN"
/gene_synonym="GluII"
/gene_synonym="KIAA0088"
/note="Glyco_hydro_31; Region: Glycosyl hydrolases family
31. Glycosyl hydrolases are key enzymes of carbohydrate
metabolism. Family 31 comprises of enzymes that are, or
similar to, alpha- galactosidases"
/db_xref="CDD:pfam01055"
BASE COUNT 842 a 976 c 1021 g 939 t
ORIGIN
1 aagatggcgg cggtagcggc agtggcggcg cgtaggaggc ggtcttgggc gtctttggta
61 ctggcttttt taggggtctg cctggggatt acccttgctg tggatagaag caactttaag
121 acctgtgaag agagttcttt ctgcaagtgt tgctggtgct agagcttcag gggcttcaaa
181 agaacatgac tcggttcagg attgatgagc tggagcctcg gcgaccccga taccgtgtac
241 cagatgtttt ggtggctgat ccaccaatag cccggctttc tgtctctggt cgtgatgaga
301 acagtgtgga gttaaccatg gctgagggac cctacaagat catcttgaca gcacggccat
361 tccgccttga cctactagag gaccgaagtc ttttgcttag tgtcaatgcc cgaggactct
421 tggagtttga gcatcagagg gcccctaggg tctcgcaagg atcaaaagac ccagctgagg
481 gcgatggggc ccagcctgag gaaacaccca gggatggcga caagccagag gagactcagg
541 ggaaggcaga gaaagatgag ccaggagcct gggaggagac attcaaaact cactctgaca
601 gcaagccgta tggccccatg tctgtgggtt tggacttctc tctgccaggc atggagcatg
661 tctatgggat ccctgagcat gcagacaacc tgaggctgaa ggtcactgag ggtggggagc
721 catatcgcct ctacaatttg gatgtgttcc agtatgagct gtacaaccca atggccttgt
781 atgggtctgt gcctgtgctc ctggcacaca accctcatcg cgacttgggc atcttctggc
841 tcaatgctgc agagacctgg gttgatatat cttccaacac tgccgggaag accctgtttg
901 ggaagatgat ggactacctg cagggctctg gggagacccc acagacagat gttcgctgga
961 tgtcagagac tggcatcatt gacgtcttcc tgctgctggg gccctccatc tctgatgttt
1021 tccggcaata tgctagtctc acaggaaccc aggcgttgcc cccactcttc tccctcggct
1081 accaccagag ctgttggaac taccgggacg aggctgatgt gctggaagtg gatcagggct
1141 ttgatgatca caacctgccc tgtgatgtca tctggctaga cattgaacat gctgatggca
1201 agcggtattt cacctgggac cccagtcgct tccctcagcc ccgcaccatg cttgagcgct
1261 tggcttctaa gaggtggaag ctggtggcca tcgtagaccc ccacatcaag gtggactccg
1321 gctaccgagt tcacgaggag ctgcggaacc tggggctgta tgttaaaacc cgggatggct
1381 ctgactatga gggctggtgc tggccaggct cagctggtta ccctgacttc actaatccca
1441 cgatgagggc ctggtgggct aacatgttca gctatgacaa ttatgagggc tcagctccca
1501 acctctttgt ctggaatgac atgaacgaac catctgtgtt caatggtcct gaggtcacca
1561 tgctcaagga tgcccagcat tatgggggct gggagcaccg ggatgtgcat aacatctatg
1621 gcctttatgt gcacatggcg actgctgatg ggctgagaca gcgctctggg ggcatggaac
1681 gcccctttgt cctggccagg gccttcttcg ctggctccca gcgctttgga gccgtgtgga
1741 caggggacaa cactgccgag tgggaccatt tgaagatctc tattcctatg tgtctcagct
1801 tggggctggt gggactttcc ttctgtgggg cggatgtggg tggcttcttc aaaaacccag
1861 agccagagct gcttgtgcgc tggtaccaga tgggtgctta ccagccattc ttccgggcac
1921 atgcccactt ggacactggg cgacgagagc catggctgtt accatctcag cacaatgata
1981 taatccgaga tgccttgggc cagcgatatt ctttgctgcc cttctggtac accctcttat
2041 atcaggccca tcgggaaggc attcctgtca tgaggcccct gtgggtgcag taccctcagg
2101 atgtgactac cttcaatata gatgatcagt acttgcttgg ggatgcgttg ctggttcacc
2161 ctgtatcaga ctctggagcc catggtgtcc aggtctatct gcctggccaa ggggaggtgt
2221 ggtatgacat tcaaagctac cagaagcatc atggtcccca gaccctgtac ctgcctgtaa
2281 ctctaagcag tatccctgtg ttccagcgtg gagggacaat cgtgcctcga tggatgcgag
2341 tgcggcggtc ttcagaatgt atgaaggatg accccatcac tctctttgtt gcacttagcc
2401 ctcagggtac agctcaagga gagctctttc tggatgatgg gcacacgttc aactatcaga
2461 ctcgccaaga gttcctgctg cgtcgattct cattctctgg caacaccctt gtctccagct
2521 cagcagaccc tgaaggacac tttgagacac caatctggat tgagcgggtg gtgataatag
2581 gggctggaaa gccagcagct gtggtactcc agacaaaagg atctccagaa agccgcctgt
2641 ccttccagca tgaccctgag acctctgtgt tggtcctgcg caagcctggc atcaatgtgg
2701 catctgattg gagtattcac ctgcgataac ccaagggatg ttctgggtta gggggaggga
2761 aggggagcat tagtgctgag agatattctt tcttctgcct tggagttcgg ccctccccag
2821 acttcactta tgctagtcta agacccagat tctgccaaca tttgggcagg atgagagggc
2881 tgaccctggg ctccaaattc ctcttgtgat ctcctcacct ctcccactcc attgatacca
2941 actctttccc ttcattcccc caacatcctg ttgctctaac tggagcacat tcacttacga
3001 acaccaggaa accacagggc ccttgtcgcc ccttctcttt cccttattta ggagccctga
3061 actcccccag agtctatcca ttcatgcctc ttgtatgttg atgccacttc ttggaagaag
3121 atgagggcaa tgagttaggg ctccttttcc ccttccctcc caccagattg ctctcccacc
3181 tttcatttct tcctccaggc tttactcccc tttttatgcc ccaccgatac actgggacca
3241 ccccttaccc cggacaggat gaatggatca aaggagtgag gttgctaaag aacatccttt
3301 tccctctcat tctacccttt tcctctcccc gattccttgt agagctgctg caattcttag
3361 aggggcagtt ctacctcctc tgtccctcgg cagaaagacg tttccacacc tcttagggga
3421 tgcgcattaa acttcttttg cccccttctt gtcccctttg aggggcactt aagatggaga
3481 aatcagttgt ggtttcagtg aatcatggtc acctgtattt attgctagga gaagcctgag
3541 ggtgggggga gatgatcatg tgtgctcggg gttggctgga agccctgggt ggggggttgg
3601 gggaggacta atggggagtc ggggaatatt tgtgggtatt ttttttactt cctcttggtt
3661 cccagctgtg acacgttttg atcaaagggg aaacaataaa gggataaacc ataaaaaaaa
3721 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaag
//