LOCUS BC064931 2345 bp mRNA linear HUM 26-JUN-2007
DEFINITION Homo sapiens primase, DNA, polypeptide 2 (58kDa), mRNA (cDNA clone
MGC:75142 IMAGE:6148494), complete cds.
ACCESSION BC064931
VERSION BC064931.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2345)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 2345)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (02-JAN-2004) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC/DCTD/DTP
cDNA Library Preparation: Life Technologies, Inc.
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: National Institutes of Health Intramural
Sequencing Center (NISC),
Gaithersburg, Maryland;
Web site: http://www.nisc.nih.gov/
Contact: nisc_mgc@nhgri.nih.gov
Akhter,N., Ayele,K., Beckstrom-Sternberg,S.M., Benjamin,B.,
Blakesley,R.W., Bouffard,G.G., Breen,K., Brinkley,C., Brooks,S.,
Dietrich,N.L., Granite,S., Guan,X., Gupta,J., Haghighi,P.,
Hansen,N., Ho,S.-L., Karlins,E., Kwong,P., Laric,P., Legaspi,R.,
Maduro,Q.L., Masiello,C., Maskeri,B., Mastrian,S.D.,McCloskey,J.C.,
McDowell,J., Pearson,R., Stantripop,S., Thomas,P.J., Touchman,J.W.,
Tsurgeon,C., Vogt,J.L., Walker,M.A., Wetherby,K.D., Wiggins,L.,
Young,A., Zhang,L.-H. and Green,E.D.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 140 Row: k Column: 1
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 41349494.
FEATURES Location/Qualifiers
source 1..2345
/db_xref="H-InvDB:HIT000261639"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:75142 IMAGE:6148494"
/tissue_type="Skin, melanotic melanoma."
/clone_lib="NIH_MGC_72"
/lab_host="DH10B"
/note="Vector: pCMV-SPORT6"
gene 1..2345
/gene="PRIM2"
/gene_synonym="p58"
/db_xref="GeneID:5558"
/db_xref="HGNC:HGNC:9370"
/db_xref="MIM:176636"
CDS 80..1609
/gene="PRIM2"
/gene_synonym="p58"
/codon_start=1
/product="primase, DNA, polypeptide 2 (58kDa)"
/protein_id="AAH64931.1"
/db_xref="GeneID:5558"
/db_xref="HGNC:HGNC:9370"
/db_xref="MIM:176636"
/translation="MEFSGRKWRKLRLAGDQRNASYPHCLQFYLQPPSENISLIEFEN
LAIDRVKLLKSVENLGVSYVKGTEQYQSKLESELRKLKFSYRENLEDEYEPRRRDHIS
HFILRLAYCQSEELRRWFIQQEMDLLRFRFSILPKDKIQDFLKDSQLQFEAISDEEKT
LREQEIVASSPSLSGLKLGFESIYKIPFADALDLFRGRKVYLEDGFAYVPLKDIVAII
LNEFRAKLSKALALTARSLPAVQSDERLQPLLNHLSHSYTGQDYSTQGNVGKISLDQI
DLLSTKSFPPCMRQLHKALRENHHLRHGGRMQYGLFLKGIGLTLEQALQFWKQEFIKG
KMDPDKFDKGYSYNIRHSFGKEGKRTDYTPFSCLKIILSNPPSQGDYHGCPFRHSDPE
LLKQKLQSYKISPGGISQILDLVKGTHYQVACQKYFEMIHNVDDCGFSLNHPNQFFCE
SQRILNGGKDIKKEPIQPETPQPKPSVQKTKDASSALASLNSSLEMDMEGLEDYFSED
S"
BASE COUNT 694 a 466 c 490 g 695 t
ORIGIN
1 atgaactctc ccgccacccg ggaacagtgg ctgccaccgt ttgtgttttc ccgagtttga
61 attcttgcag gtgaccaaga tggagttttc tggaagaaag tggaggaagc tgaggttggc
121 aggtgaccag aggaatgctt cctaccctca ttgccttcag ttttacttgc agccaccttc
181 tgaaaacata tctttaatag aatttgaaaa cttggctatt gatagagtta aattgttaaa
241 atcagttgaa aatcttggag tgagctatgt gaaaggaact gaacaatacc agagtaagtt
301 ggagagtgag cttcggaagc tcaagttttc ctacagagaa aacttagaag atgaatatga
361 accacgaaga agagatcata tttctcattt tattttgcgg cttgcttatt gccagtctga
421 agaacttaga cgctggttca ttcaacaaga aatggatctc cttcgattta gatttagtat
481 tttacccaag gataaaattc aggatttctt aaaggatagc caattgcagt ttgaggctat
541 aagtgatgaa gagaagactc ttcgagaaca ggagattgtt gcctcatcac caagtttaag
601 tggacttaag ttggggttcg agtccattta taagatccct tttgctgatg ctctggattt
661 gtttcgagga aggaaagtct atttggaaga tggctttgct tacgtaccac ttaaggacat
721 tgtggcaatc atcctgaatg aatttagagc caaactgtcc aaggctttgg cattaacagc
781 caggtccttg cctgctgtgc agtctgatga aagacttcag cctctgctca atcacctcag
841 tcattcctac actggccaag attacagtac ccagggaaat gttgggaaga tttctttaga
901 tcagattgat ttgctttcta ccaaatcctt cccaccttgc atgcgtcagt tacataaagc
961 cttgcgggaa aatcaccatc ttcgtcatgg aggccgaatg cagtatggcc tatttctgaa
1021 gggcattggt ttaactttgg aacaggcatt gcagttctgg aagcaagaat ttatcaaagg
1081 aaagatggat ccagacaagt ttgataaagg ttactcttac aacatccgtc acagctttgg
1141 aaaggaaggc aagaggacag actatacacc tttcagttgc ctgaagatta ttctgtccaa
1201 tccaccaagc caaggggatt atcatgggtg cccattccgt cacagtgatc cagagctgct
1261 gaagcaaaag ttgcagtcat acaagatctc tcctggaggg ataagccaga ttttggattt
1321 agtaaagggg acacattacc aggtagcctg tcaaaaatac tttgagatga tacacaatgt
1381 ggatgattgt ggcttttctt tgaatcatcc taatcagttc ttttgtgaga gccaacgtat
1441 tctaaatggt ggtaaagaca taaagaagga acctatccaa ccagaaactc ctcaacccaa
1501 accaagtgtc cagaaaacca aggatgcatc atctgctctg gcctctttaa attcctctct
1561 ggaaatggat atggaaggac tagaagatta ctttagtgaa gattcttagg cagttttata
1621 accctttttc ctcaatagcc tgtttcctgt ttttaagatt ttgcctttgt tgttgaaaaa
1681 gggtttcact ctgtcaccaa ggcttagtgc agtgacacaa ttacagctga ttgcagcctt
1741 gaccttccca gctcaagtga tcctcctacc tcagcctccc aagtagttag gaccacaggt
1801 gtgcacctca tatccagata atttttttca attttttttt gtagaggtgg ggggtctccc
1861 tatgttgccc aggcagatct cagactcctg ggctcaagcg atcctcacac ctcagcgtcc
1921 cagagtgctg ggattacggt tgtgagccac tgtgcctggc cttttttttt ttttttaacc
1981 tttttgttta acttctctct tcactgcatc ccaatccatc tacaggcatg cacacttatt
2041 aggaaaggag gtttgaggta acaacagaga ctttcactat attttgcttt gacagaagga
2101 aagaggagga gtttctatta aaatctgtca cttgagtgat gtcatttaag tcctatttta
2161 ggagataaaa acagctttgg ggactggtta aagtccccca gaaactacaa taaagaacaa
2221 cttttgtttt aactcttaat cactttgtaa ttttgactca atccttttct ggaccatttt
2281 tgttaataaa tatcaaagtg taaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
2341 aaaaa
//