LOCUS BC064838 2940 bp mRNA linear HUM 18-AUG-2008
DEFINITION Homo sapiens makorin ring finger protein 1, mRNA (cDNA clone
MGC:74655 IMAGE:6173715), complete cds.
ACCESSION BC064838
VERSION BC064838.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2940)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 2940)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (02-JAN-2004) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC
cDNA Library Preparation: Life Technologies, Inc.
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Genome Sequence Centre,
BC Cancer Agency, Vancouver, BC, Canada
info@bcgsc.bc.ca
Martin Hirst, Thomas Zeng, Ryan Morin, Michelle Moksa, Johnson
Pang, Diana Mah, Jing Wang, Kieth Fichter, Eric Chuah, Allen
Delaney, Rob Kirkpatrick, Agnes Baross, Sarah Barber, Mabel
Brown-John, Steve S. Chand, William Chow, Ryan Babakaiff, Dave
Wong, Corey Matsuo, Jaclyn Beland, Susan Gibson, Luis delRio, Ruth
Featherstone, Malachi Griffith, Obi Griffith, Ran Guin, Nancy Liao,
Kim MacDonald, Mike R. Mayo, Josh Moran, Diana Palmquist, JR
Santos, Duane Smailus, Jeff Stott, Miranda Tsai, George Yang,
Jacquie Schein, Asim Siddiqui,Steven Jones, Rob Holt, Marco Marra.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 141 Row: a Column: 4
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 21359891.
FEATURES Location/Qualifiers
source 1..2940
/db_xref="H-InvDB:HIT000261595"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:74655 IMAGE:6173715"
/tissue_type="Uterus, leiomyosarcoma"
/clone_lib="NIH_MGC_71"
/lab_host="DH10B"
/note="Vector: pCMV-SPORT6"
gene 1..2940
/gene="MKRN1"
/gene_synonym="RNF61"
/db_xref="GeneID:23608"
/db_xref="HGNC:HGNC:7112"
/db_xref="MIM:607754"
CDS 4..1452
/gene="MKRN1"
/gene_synonym="RNF61"
/codon_start=1
/product="makorin ring finger protein 1"
/protein_id="AAH64838.1"
/db_xref="GeneID:23608"
/db_xref="HGNC:HGNC:7112"
/db_xref="MIM:607754"
/translation="MAEAATPGTTATTSGAGAAAATAAAASPTPIPTVTAPSLGAGGG
GGGSDGSGGGWTKQVTCRYFMHGVCKEGDNCRYSHDLSDSPYSVVCKYFQRGYCIYGD
RCRYEHSKPLKQEEATATELTTKSSLAASSSLSSIVGPLVEMNTGEAESRNSNFATVG
AGSEDWVNAIEFVPGQPYCGRTAPSCTEAPLQGSVTKEESEKEQTAVETKKQLCPYAA
VGECRYGENCVYLHGDSCDMCGLQVLHPMDAAQRSQHIKSCIEAHEKDMELSFAVQRS
KDMVCGICMEVVYEKANPSERRFGILSNCNHTYCLKCIRKWRSAKQFESKIIKSCPEC
RITSNFVIPSEYWVEEKEEKQKLILKYKEAMSNKACRYFDEGRGSCPFGGNCFYKHAY
PDGRREEPQRQKVGTSSRYRAQRRNHFWELIEERENSNPFDNDEEEVVTFELGEMLLM
LLAAGGDDELTDSEDEWDLFHDELEDFYDLDL"
BASE COUNT 778 a 615 c 767 g 780 t
ORIGIN
1 gtaatggcgg aggctgcaac tcccggaaca acagccacaa catcaggagc aggagcggca
61 gcggcgacgg cggcagcagc ctcccccacc ccgatcccca cagtcaccgc cccgtccctg
121 ggggcgggcg gagggggcgg cggcagcgac ggcagcggcg gcggctggac taaacaggtc
181 acctgcaggt attttatgca tggggtttgt aaggaaggag acaactgtcg ctactcgcat
241 gacctctctg acagtccgta tagtgtagtg tgcaagtatt ttcagcgagg gtactgtatt
301 tatggagacc gctgcagata tgaacatagc aaaccattga aacaggaaga agcaactgct
361 acagagctaa ctacaaagtc atcccttgct gcttcctcaa gtctctcatc gatagttgga
421 ccacttgttg aaatgaatac aggcgaagct gagtcaagaa attcaaactt tgcaactgta
481 ggagcaggtt cagaggactg ggtgaatgct attgagtttg ttcctgggca accctactgt
541 ggccgtactg cgccttcctg cactgaagca cccctgcagg gctcagtgac caaggaagaa
601 tcagagaaag agcaaaccgc cgtggagaca aagaagcagc tgtgccccta tgctgcagtg
661 ggagagtgcc gatacgggga gaactgtgtg tatctccacg gagattcttg tgacatgtgt
721 gggctgcagg tcctgcatcc aatggatgct gcccagagat cgcagcatat caaatcgtgc
781 attgaggccc atgagaagga catggagctc tcatttgccg tgcagcgcag caaggacatg
841 gtgtgtggga tctgcatgga ggtggtctat gagaaagcca accccagtga gcgccgcttc
901 gggatcctct ccaactgcaa ccacacctac tgtctcaagt gcattcgcaa gtggaggagt
961 gctaagcaat ttgagagcaa gatcataaag tcctgcccag aatgccggat cacatctaac
1021 tttgtcattc caagtgagta ctgggtggag gagaaagaag agaagcagaa actcattctg
1081 aaatacaagg aggcaatgag caacaaggcg tgcaggtatt ttgatgaagg acgtgggagc
1141 tgcccatttg gagggaactg tttttacaag catgcgtacc ctgatggccg tagagaggag
1201 ccacagagac agaaagtggg aacatcaagc agataccggg cccaacgaag gaaccacttc
1261 tgggaactca ttgaggaaag agagaacagc aacccctttg acaacgatga agaagaggtt
1321 gtcacctttg agctgggcga gatgttgctt atgcttttgg ctgcaggtgg ggacgacgaa
1381 ctaacagact ctgaagatga gtgggacttg tttcatgatg agctggaaga tttttatgac
1441 ttggatctat agcaaccttg cgtggcgtgt gaactggtct gctgacctca gacagcagct
1501 gtcccctgtg gtggtgtggc agtgcctgtg ttctctccta ggcaggcctc tcaactccag
1561 gtgctgtcct aagaattttt acccagggcc tgtcttctca acccctcacc tttccctgag
1621 gagtgtgttg ttttccctgt tgaaaaaagt tacaaaaata aatcttaaag ttagtttttt
1681 gtaacacgaa tttaactgtc agacagttag tgtaggtgtg ttgcgtcatc tgttttcaac
1741 cagattgcat ttatggactt ttcacacact cattttgagg accccaggtt caaaagtaaa
1801 agcagtggcc ctgctttggg gtccaagaat aggagtgatg ggtgaaggga cctaagctgg
1861 ccaatagccc tctgccccag acatgggatg tggatccttg aggtttctgg tgaaatctgc
1921 acatctgtgt ttttatatct gttccctacc ctgtaatccc taccacgtgc acttgttctg
1981 tggttttggt ctcttgttta attgcacaca agtaatacta ctgggtaacc agaatcaggt
2041 gtgaatgtgt tgagattttt ttactgtttt gcatgatagg aaaattgaga aagaatacgt
2101 ataaaagata gagaggcata acatcaatgc agagttggaa gttggctccc aagggctgac
2161 atggtgtgag tgtgtgggtg tgtgataagc ttctcatccc tgcatagatg cagtattctt
2221 agccttagta gaaaaacctg gtttagtggt ttaagccttg tgtggcagat agatcttaaa
2281 gggcaaagca gtatattggt agttgtcaat atagcagtgc tagctctgtc tatataaata
2341 gagaaatggg gttagccata gaggttaaaa ctacctggtt atcccatata ataacacaaa
2401 ctgggtcttg gatacacagt tgtatttaat gttttacgat ctagcctttc cagtacaggc
2461 actttctgag aaacctttgt cctcacttga ggcattttgt tgtcgggttt ttgtgtttgt
2521 ttttgtgggt atttgcctca ttccacccct gagctttcag gtagacagac gtgattcaaa
2581 actctgttct aaggtgttta ttgtagtgga gtaatgggtt tgcagtgata agtcatactt
2641 ttccaccgaa agggagggct tgggaatccc tgagattagc taaagttaag ttgttggaag
2701 aattccttga ttggaaattg tacctttgtg ttttgttgct ctgtttcctg aaaataactc
2761 ggggatgctc ctggtttgtc catctactgc tttgattcct tggatcccac ccattctttc
2821 actttaagaa aaaacaaata attgttgcag aggtctctgt attttgcagc tgcccttttg
2881 taagaagcac ttttcccaaa taaaacaatt aaaaaaaaag ttggcaaaaa aaaaaaaaaa
//