LOCUS BC064370 7276 bp mRNA linear HUM 15-JUL-2006
DEFINITION Homo sapiens PRP8 pre-mRNA processing factor 8 homolog (S.
cerevisiae), mRNA (cDNA clone MGC:74762 IMAGE:5587081), complete
cds.
ACCESSION BC064370
VERSION BC064370.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 7276)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 7276)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (15-DEC-2003) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC
cDNA Library Preparation: Life Technologies, Inc.
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: National Institutes of Health Intramural
Sequencing Center (NISC),
Gaithersburg, Maryland;
Web site: http://www.nisc.nih.gov/
Contact: nisc_mgc@nhgri.nih.gov
Akhter,N., Ayele,K., Beckstrom-Sternberg,S.M., Benjamin,B.,
Blakesley,R.W., Bouffard,G.G., Breen,K., Brinkley,C., Brooks,S.,
Dietrich,N.L., Granite,S., Guan,X., Gupta,J., Haghighi,P.,
Hansen,N., Ho,S.-L., Karlins,E., Kwong,P., Laric,P., Legaspi,R.,
Maduro,Q.L., Masiello,C., Maskeri,B., Mastrian,S.D.,McCloskey,J.C.,
McDowell,J., Pearson,R., Stantripop,S., Thomas,P.J., Touchman,J.W.,
Tsurgeon,C., Vogt,J.L., Walker,M.A., Wetherby,K.D., Wiggins,L.,
Young,A., Zhang,L.-H. and Green,E.D.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 139 Row: e Column: 11
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 17999536.
FEATURES Location/Qualifiers
source 1..7276
/db_xref="H-InvDB:HIT000261333"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:74762 IMAGE:5587081"
/tissue_type="Testis, embryonal carcinoma"
/clone_lib="NIH_MGC_92"
/lab_host="DH10B"
/note="Vector: pCMV-SPORT6"
gene 1..7276
/gene="PRPF8"
/gene_synonym="HPRP8"
/gene_synonym="PRP8"
/gene_synonym="PRPC8"
/db_xref="GeneID:10594"
/db_xref="HGNC:HGNC:17340"
/db_xref="MIM:607300"
CDS 80..7087
/gene="PRPF8"
/gene_synonym="HPRP8"
/gene_synonym="PRP8"
/gene_synonym="PRPC8"
/codon_start=1
/product="PRP8 pre-mRNA processing factor 8 homolog (S.
cerevisiae)"
/protein_id="AAH64370.1"
/db_xref="GeneID:10594"
/db_xref="HGNC:HGNC:17340"
/db_xref="MIM:607300"
/translation="MAGVFPYRGPGNPVPGPLAPLPDYMSEEKLQEKARKWQQLQAKR
YAEKRKFGFVDAQKEDMPPEHVRKIIRDHGDMTNRKFRHDKRVYLGALKYMPHAVLKL
LENMPMPWEQIRDVPVLYHITGAISFVNEIPWVIEPVYISQWGSMWIMMRREKRDRRH
FKRMRFPPFDDEEPPLDYADNILDVEPLEAIQLELDPEEDAPVLDWFYDHQPLRDSRK
YVNGSTYQHWQFTLPMMSTLYRLANQLLTDLVDDNYFYLFDLKAFFTSKALNMAIPGG
PKFEPLVRDINLQDEDWNEFNDINKIIIRQPIRTEYKIAFPYLYNNLPHHVHLTWYHT
PNVVFIKTEDPDLPAFYFDPLINPISHRHSVKSQEPLPDDDEEFELPEFVEPFLKDTP
LYTDNTANGIALLWAPRPFNLRSGRTRRALDIPLVKNWYREHCPAGQPVKVRVSYQKL
LKYYVLNALKHRPPKAQKKRYLFRSFKATKFFQSTKLDWVEVGLQVCRQGYNMLNLLI
HRKNLNYLHLDYNFNLKPVKTLTTKERKKSRFGNAFHLCREVLRLTKLVVDSHVQYRL
GNVDAFQLADGLQYIFAHVGQLTGMYRYKYKLMRQIRMCKDLKHLIYYRFNTGPVGKG
PGCGFWAAGWRVWLFFMRGITPLLERWLGNLLARQFEGRHSKGVAKTVTKQRVESHFD
LELRAAVMHDILDMMPEGIKQNKARTILQHLSEAWRCWKANIPWKVPGLPTPIENMIL
RYVKAKADWWTNTAHYNRERIRRGATVDKTVCKKNLGRLTRLYLKAEQERQHNYLKDG
PYITAEEAVAVYTTTVHWLESRRFSPIPFPPLSYKHDTKLLILALERLKEAYSVKSRL
NQSQREELGLIEQAYDNPHEALSRIKRHLLTQRAFKEVGIEFMDLYSHLVPVYDVEPL
EKITDAYLDQYLWYEADKRRLFPPWIKPADTEPPPLLVYKWCQGINNLQDVWETSEGE
CNVMLESRFEKMYEKIDLTLLNRLLRLIVDHNIADYMTAKNNVVINYKDMNHTNSYGI
IRGLQFASFIVQYYGLVMDLLVLGLHRASEMAGPPQMPNDFLSFQDIATEAAHPIRLF
CRYIDRIHIFFRFTADEARDLIQRYLTEHPDPNNENIVGYNNKKCWPRDARMRLMKHD
VNLGRAVFWDIKNRLPRSVTTVQWENSFVSVYSKDNPNLLFNMCGFECRILPKCRTSY
EEFTHKDGVWNLQNEVTKERTAQCFLRVDDESMQRFHNRVRQILMASGSTTFTKIVNK
WNTALIGLMTYFREAVVNTQELLDLLVKCENKIQTRIKIGLNSKMPSRFPPVVFYTPK
ELGGLGMLSMGHVLIPQSDLRWSKQTDVGITHFRSGMSHEEDQLIPNLYRYIQPWESE
FIDSQRVWAEYALKRQEAIAQNRRLTLEDLEDSWDRGIPRINTLFQKDRHTLAYDKGW
RVRTDFKQYQVLKQNPFWWTHQRHDGKLWNLNNYRTDMIQALGGVEGILEHTLFKGTY
FPTWEGLFWEKASGFEESMKWKKLTNAQRSGLNQIPNRRFTLWWSPTINRANVYVGFQ
VQLDLTGIFMHGKIPTLKISLIQIFRAHLWQKIHESIVMDLCQVFDQELDALEIETVQ
KETIHPRKSYKMNSSCADILLFASYKWNVSRPSLLADSKDVMDSTTTQKYWIDIQLRW
GDYDSHDIERYARAKFLDYTTDNMSIYPSPTGVLIAIDLAYNLHSAYGNWFPGSKPLI
QQAMAKIMKANPALYVLRERIRKGLQLYSSEPTEPYLSSQNYGELFSNQIIWFVDDTN
VYRVTIHKTFEGNLTTKPINGAIFIFNPRTGQLFLKIIHTSVWAGQKRLGQLAKWKTA
EEVAALIRSLPVEEQPKQIIVTRKGMLDPLEVHLLDFPNIVIKGSELQLPFQACLKVE
KFGDLILKATEPQMVLFNLYDDWLKTISSYTAFSRLILILRALHVNNDRAKVILKPDK
TTITEPHHIWPTLTDEEWIKVEVQLKDLILADYGKKNNVNVASLTQSEIRDIILGMEI
SAPSQQRQQIAEIEKQTKEQSQLTATQTRTVNKHGDEIITSTTSNYETQTFSSKTEWR
VRAISAANLHLRTNHIYVSSDDIKETGYTYILPKNVLKKFICISDLRAQIAGYLYGVS
PPDNPQVKEIRCIVMVPQWGTHQTVHLPGQLPQHEYLKEMEPLGWIHTQPNESPQLSP
QDVTTHAKIMADNPSWDGEKTIIITCSFTPGSCTLTAYKLTPSGYEWGRQNTDKGNNP
KGYLPSHYERVQMLLSDRFLGFFMVPAQSSWNYNFMGVRHDPNMKYELQLANPKEFYH
EVHRPSHFLNFALLQEGEVYSADREDLYA"
BASE COUNT 1841 a 1975 c 1821 g 1639 t
ORIGIN
1 cggagcggac gcagtctggc cgccattgcg ctgcggggaa agcggcctct tgtgtgaggg
61 cctgtgggat tctccggata tggccggagt gtttccttat cgagggccgg gtaacccggt
121 gcctggccct ctagccccgc taccggacta catgtcggag gagaagctgc aggagaaagc
181 tcgaaaatgg cagcaattgc aggccaagcg ctatgcagaa aagcggaagt ttgggtttgt
241 ggatgcccag aaggaagaca tgcccccaga acatgtcagg aagatcattc gagaccatgg
301 agacatgacc aacaggaagt tccgccatga caaaagggtt tacttgggtg ccctaaagta
361 catgccccac gcagtcctca aactcctgga gaacatgcct atgccttggg agcagattcg
421 ggatgtgcct gtgctgtacc acatcactgg agccatttcc ttcgtcaatg agattccctg
481 ggtcattgaa cctgtctaca tctcccagtg ggggtcaatg tggattatga tgcgccgaga
541 aaaaagagat aggaggcatt tcaagaggat gcgttttccc ccttttgatg atgaggagcc
601 gcccttggac tatgctgaca acatcctaga tgttgagcca ctggaggcca ttcagctaga
661 gctggaccct gaggaggacg cccctgtgtt ggactggttc tatgaccacc agccgttgag
721 ggacagcagg aagtatgtaa atggctccac ttaccagcac tggcagttca cactacctat
781 gatgtcgact ctctaccgcc tggctaatca gctcctgaca gacttggtgg atgacaacta
841 cttctacctg tttgatttga aggccttctt tacgtccaag gcactcaata tggccattcc
901 tggaggcccc aaatttgaac ctcttgttcg agacatcaac ctacaggatg aagactggaa
961 tgaattcaat gatattaaca agattatcat ccggcagcct atccgcactg agtacaagat
1021 tgcttttcct tacttgtaca acaatcttcc acaccatgtc cacctcacct ggtaccatac
1081 tcccaatgtt gtattcatca aaactgagga tcctgacttg ccagctttct actttgaccc
1141 tttgatcaac ccaatctccc ataggcactc agtcaagagc caggaaccat tgccggatga
1201 tgatgaggaa tttgagctcc cggagtttgt ggagcccttc ctgaaggaca cacccctcta
1261 tacagacaat acagccaatg gcattgccct gctctgggcc ccgcggccct tcaacctacg
1321 ctctggtcgc acccgtcggg ccctggacat accccttgtc aagaactggt atcgggagca
1381 ttgtcctgcc gggcagcctg tgaaagtgag ggtctcctac cagaagctgc ttaagtacta
1441 tgtgctgaat gccctgaagc atcggccccc taaggctcaa aagaagaggt atttgttccg
1501 ctccttcaaa gccaccaaat tctttcagtc cacaaagctg gactgggtgg aggttgggct
1561 ccaggtttgc cgccagggct acaacatgct caaccttctc attcaccgca aaaacctcaa
1621 ctacctgcac ctggactaca acttcaacct caagcctgtg aaaacgctca ccaccaagga
1681 aagaaagaaa tctcgttttg ggaatgcttt ccacctgtgt cgggaagttc tgcgtttgac
1741 taagctggtg gtggatagtc acgtgcagta tcggctgggc aatgtggatg ccttccagct
1801 ggcagatgga ttgcagtata tatttgccca tgttgggcag ttgacgggca tgtatcgata
1861 caaatacaag ctgatgcgac agattcgcat gtgcaaggac ctgaagcatc tcatctatta
1921 tcgtttcaac acgggccctg tagggaaggg tcctggctgt ggcttctggg ctgccggttg
1981 gcgagtctgg ctctttttca tgcgtggcat taccccttta ttagagcgat ggcttggcaa
2041 cctcctggcc cggcagtttg aaggtcgaca ctcaaagggg gtggcaaaga cagtaacaaa
2101 gcagcgagtg gagtcacatt ttgaccttga gctgcgggca gctgtgatgc atgatattct
2161 ggacatgatg cctgagggga tcaagcagaa caaggcccgg acaatcctgc agcacctcag
2221 tgaagcctgg cgctgctgga aagccaacat tccctggaag gtccctgggc tgccgacgcc
2281 catagagaat atgatccttc gatacgtgaa ggccaaggct gactggtgga ccaacactgc
2341 ccactacaac cgagaacgga tccgccgagg ggccactgtg gacaagactg tttgtaaaaa
2401 gaatctgggc cgcctcaccc ggctctatct gaaggcagaa caggagcggc agcacaacta
2461 cctgaaggac gggccttaca tcacagcgga ggaagcagtg gcagtatata ccaccacagt
2521 gcattggttg gaaagccgca ggttttcacc catcccattc cccccactct cctataagca
2581 tgacaccaag ttgctcatct tggcattgga gcggctcaag gaagcttata gtgtgaagtc
2641 tcggttgaac cagtctcaga gggaggagct aggtctgatc gagcaggcct acgataaccc
2701 ccacgaggcg ctgtcccgca tcaagcgtca cctcctcaca cagagagcct tcaaagaggt
2761 gggcattgag ttcatggatc tgtatagcca cctcgttcca gtatatgatg ttgagcccct
2821 ggagaagata actgatgctt acctggacca gtacctgtgg tatgaagccg acaagcgccg
2881 cctgttccca ccctggatta agcctgcaga cacagaacca cctccgctgc ttgtttacaa
2941 gtggtgtcaa ggcatcaata acctgcagga cgtgtgggag acgagtgaag gcgagtgcaa
3001 tgtcatgctg gaatcccgct ttgagaagat gtatgagaag atcgacttga ctctgctcaa
3061 caggctgctg cgcctcatcg tggaccacaa catagccgac tacatgacag ccaagaacaa
3121 cgtcgtcatc aactataagg acatgaacca tacgaattca tatgggatca tcagaggcct
3181 gcagtttgcc tcattcatcg tgcagtatta tggcctggtg atggatttgc ttgtattggg
3241 attgcaccgg gccagtgaga tggctgggcc ccctcagatg ccaaatgact ttctcagttt
3301 ccaggacata gccactgagg ctgcccaccc catccgtctc ttctgcagat acattgatcg
3361 catccatatt tttttcaggt tcacagcaga tgaggctcgg gacctgattc aacgttacct
3421 gacagagcac cctgacccca ataatgaaaa catcgttggc tataataaca agaagtgctg
3481 gccccgagat gcccgcatgc gcctcatgaa acatgatgtt aacttaggcc gggcggtatt
3541 ctgggacatc aagaaccgct tgccacggtc agtgactaca gttcagtggg agaacagctt
3601 cgtgtctgtg tacagtaagg acaaccccaa cctgctgttc aacatgtgtg gcttcgagtg
3661 ccgcatcctg cctaagtgcc gcaccagcta tgaggagttc acccacaagg acggggtctg
3721 gaacctgcag aatgaggtta ctaaggagcg cacagctcag tgtttcctgc gtgtggacga
3781 tgagtcaatg cagcgcttcc acaaccgcgt gcgtcagatt ctcatggcct ctgggtccac
3841 caccttcacc aagattgtga ataagtggaa tacagctctc attggcctta tgacatactt
3901 tcgggaggct gtggtgaaca cccaagagct cttggactta ctggtgaagt gtgagaacaa
3961 aatccagaca cgtatcaaga ttggactcaa ctccaagatg ccaagtcggt tccccccggt
4021 tgtgttctac acccctaagg agttgggtgg actcggcatg ctctcaatgg gccatgtgct
4081 catcccccaa tccgacctca ggtggtccaa acagacagat gtaggtatca cacactttcg
4141 ttcaggaatg agccatgaag aagaccagct cattcccaac ttgtaccgct acatacagcc
4201 atgggagagc gagttcattg attctcagcg ggtctgggct gagtacgcac tcaagaggca
4261 agaggccatt gctcagaaca gacgcctgac tttagaagac ctagaagatt catgggatcg
4321 tggcattcct cgaatcaata ccctcttcca gaaggaccgg cacacactgg cttatgataa
4381 gggctggcgt gtcagaactg actttaagca gtatcaggtt ttgaagcaga atccgttctg
4441 gtggacacac cagcggcatg atgggaagct ctggaacctg aacaactacc gtacagacat
4501 gatccaggcc ctgggcggtg tggaaggcat tctggaacac acactcttta agggcactta
4561 cttccctacc tgggaggggc ttttctggga gaaggccagt ggctttgagg aatctatgaa
4621 gtggaagaag ctaactaatg ctcagcgatc aggactgaac cagattccca atcgtagatt
4681 caccctctgg tggtccccga ccattaatcg agccaatgta tatgtaggct ttcaggtgca
4741 gctagacctg acgggtatct tcatgcacgg caagatcccc acgctgaaga tctctctcat
4801 ccagatcttc cgagctcact tgtggcagaa gatccatgag agcattgtta tggacttatg
4861 tcaggtgttt gaccaggaac ttgatgcact ggaaattgag acagtacaaa aggagacaat
4921 ccatccccga aagtcatata agatgaactc ttcctgtgca gatatcctgc tctttgcctc
4981 ctataagtgg aatgtctccc ggccctcatt gctggctgac tccaaggatg tgatggacag
5041 caccaccacc cagaaatact ggattgacat ccagttgcgc tggggggact atgattccca
5101 cgacattgag cgctacgccc gggccaagtt cctggactac accaccgaca acatgagtat
5161 ctacccttcg cccacaggtg tactcatcgc cattgacctg gcctataact tgcacagtgc
5221 ctatggaaac tggttcccag gcagcaagcc tctcatacaa caggccatgg ccaagatcat
5281 gaaggcaaac cctgccctgt atgtgttacg tgaacggatc cgcaaggggc tacagctcta
5341 ttcatctgaa cccactgagc cttatttgtc ttctcagaac tatggtgagc tcttctccaa
5401 ccagattatc tggtttgtgg atgacaccaa cgtctacaga gtgactattc acaagacctt
5461 tgaagggaac ttgacaacca agcccatcaa cggagccatc ttcatcttca acccacgcac
5521 agggcagctg ttcctcaaga taatccacac gtccgtgtgg gcgggacaga agcgtttggg
5581 gcagttggct aagtggaaga cagctgagga ggtggccgcc ctgatccgat ctctgcctgt
5641 ggaggagcag cccaagcaga tcattgtcac caggaagggc atgctggacc cactggaggt
5701 gcacttactg gacttcccca atattgtcat caaaggatcg gagctccaac tccctttcca
5761 ggcgtgtctc aaggtggaaa aattcgggga tctcatcctt aaagccactg agccccagat
5821 ggttctcttc aacctctatg acgactggct caagactatt tcatcttaca cggccttctc
5881 ccgtctcatc ctgattctgc gtgccctaca tgtgaacaac gatcgggcaa aagtgatcct
5941 gaagccagac aagactacta ttacagaacc acaccacatc tggcccactc tgactgacga
6001 agaatggatc aaggtcgagg tgcagctcaa ggatctgatc ttggctgact acggcaagaa
6061 aaacaatgtg aacgtggcat cactgacaca atcagaaatt cgagacatca tcctgggtat
6121 ggagatctcg gcaccgtcac agcagcggca gcagatcgct gagatcgaga agcagaccaa
6181 ggaacaatcg cagctgacgg caacacagac tcgcactgtc aacaagcatg gcgatgagat
6241 catcacctcc accaccagca actatgagac ccagactttc tcatccaaga ctgagtggag
6301 ggtcagggcc atctctgctg ccaacctgca cctaaggacc aatcacatct atgtttcatc
6361 tgacgacatc aaggagactg gctacaccta catccttccc aagaatgtgc ttaagaagtt
6421 catctgcata tctgaccttc gggcccaaat tgcaggatac ctatatgggg tgagcccacc
6481 agataacccc caggtgaagg agatccgctg cattgtgatg gtgccgcagt ggggcactca
6541 ccagaccgtg cacctgcctg gccagctgcc ccagcatgag tacctcaagg agatggaacc
6601 cttaggttgg atccacactc agcccaatga gtccccgcag ttatcacccc aggatgtcac
6661 cacccatgcc aagatcatgg ctgacaaccc atcttgggat ggcgagaaga ccattatcat
6721 cacatgcagc ttcacgccag gctcctgtac actgacggcc tacaagctga cccccagtgg
6781 ctacgaatgg ggccgccaga acacagacaa gggcaacaac cccaagggct acctgccttc
6841 acactatgag agggtgcaga tgctgctgtc ggaccgtttc cttggcttct tcatggtccc
6901 tgcccagtcc tcgtggaact acaacttcat gggtgttcgg catgacccca acatgaaata
6961 tgagctacag ctggcgaacc ccaaagagtt ctaccacgag gtgcacaggc cctctcactt
7021 cctcaacttt gctctcctgc aggaggggga ggtttactct gcggatcggg aggacctgta
7081 tgcctgaccg tttccctgcc tcctgcttca gcctcccgag gccgaagcct cagcccctcc
7141 agacaggccg ctgacattca gcagtttggc ctctttccct ctgtctgtgc ttgtgttgtt
7201 gacctcctga tggcttgtca tcctgaataa aatataataa taaattttgt ataaatagga
7261 aaaaaaaaaa aaaaaa
//