LOCUS BC064027 1731 bp mRNA linear HUM 18-JAN-2005
DEFINITION Homo sapiens dihydropyrimidine dehydrogenase, mRNA (cDNA clone
IMAGE:6173084), complete cds.
ACCESSION BC064027
VERSION BC064027.1
KEYWORDS .
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1731)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 1731)
AUTHORS Director MGC Project.
TITLE Direct Submission
JOURNAL Submitted (11-DEC-2003) National Institutes of Health, Mammalian
Gene Collection (MGC), Cancer Genomics Office, National Cancer
Institute, 31 Center Drive, Room 11A03, Bethesda, MD 20892-2590,
USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC
cDNA Library Preparation: Life Technologies, Inc.
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Genome Sequence Centre,
BC Cancer Agency, Vancouver, BC, Canada
info@bcgsc.bc.ca
Steve Jones, Sarah Barber, Mabel Brown-John, Yaron Butterfield,
Andy Chan, Steve S. Chand, William Chow, Alison Cloutier, Ruth
Featherstone, Malachi Griffith, Obi Griffith, Ran Guin, Nancy Liao,
Kim MacDonald, Amara Masson, Mike R. Mayo, Josh Moran, Ryan Morin,
Teika Olson, Diana Palmquist, Anca Petrescu, Anna Liisa Prahbu,
Parvaneh Saeedi, JR Santos, Angelique Schnerch, Ursula Skalska,
Duane Smailus, Jeff Stott, Miranda Tsai, George Yang, Jacquie
Schein, Asim Siddiqui, Rob Holt, Marco Marra.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 141 Row: a Column: 3
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 4557874
This clone has the following problem: The cds is short compared to
the longest cds in the locus.
FEATURES Location/Qualifiers
source 1..1731
/db_xref="H-InvDB:HIT000261272"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="IMAGE:6173084"
/tissue_type="Uterus, leiomyosarcoma"
/clone_lib="NIH_MGC_71"
/lab_host="DH10B"
/note="Vector: pCMV-SPORT6"
gene 1..1731
/gene="DPYD"
/gene_synonym="DHP"
/gene_synonym="DPD"
/db_xref="GeneID:1806"
/db_xref="MIM:274270"
CDS 64..585
/gene="DPYD"
/gene_synonym="DHP"
/gene_synonym="DPD"
/codon_start=1
/product="DPYD protein"
/protein_id="AAH64027.1"
/db_xref="GeneID:1806"
/db_xref="MIM:274270"
/translation="MAPVLSKDSADIESILALNPRTQTHATLCSTSAKKLDKKHWKRN
PDKNCFNCEKLENNFDDIKHTTLGERGALREAMRCLKCADAPCQKSCPTNLDIKSFIT
SIANKNYYGAAKMIFSDNPLGLTCGMVCPTSDLCVGGCNLYATEEGPINIGGLQQFAT
ETLILAFSLMNHL"
BASE COUNT 527 a 310 c 358 g 536 t
ORIGIN
1 ggctggagct tgaggacgca aggagggttt gtcactggca gactcgagac tgtaggcact
61 gccatggccc ctgtgctcag taaggactcg gcggacatcg agagtatcct ggctttaaat
121 cctcgaacac aaactcatgc aactctgtgt tccacttcgg ccaagaaatt agacaagaaa
181 cattggaaaa gaaatcctga taagaactgc tttaattgtg agaagctgga gaataatttt
241 gatgacatca agcacacgac tcttggtgag cgaggagctc tccgagaagc aatgagatgc
301 ctgaaatgtg cagatgcccc gtgtcagaag agctgtccaa ctaatcttga tattaaatca
361 ttcatcacaa gtattgcaaa caagaactat tatggagctg ctaagatgat attttctgac
421 aacccacttg gtctgacttg tggaatggta tgtccaacct ctgatctttg tgtaggtgga
481 tgcaatttat atgccactga agagggaccc attaatattg gtggattgca gcaatttgct
541 actgagactt tgatcctggc tttctcttta atgaatcatt tgtaaagact ttgattcttc
601 agacactctt gggctctctg acgtctccta tttggagtca acaagcaggt taattcccat
661 gtatcattta aatgatcagc ttatatgaag ggtatagtaa gattttataa tctttggaga
721 ctagttggct agaaggtcaa attaagtgcc cgtcaaccta gatttttagt taatgactta
781 ctgatctggt tttgtcagca taaacacaat gtgttctttc attttgggac ccttcccata
841 atattcctca agcttaagtc tcactttgct cttagtaata aatcagggtt attcccttcc
901 tgctttctga caggttaatt ctaaagaaga gctcctggga ataagaaaat agaaacaatt
961 cgaggttgtg gattgttgca ataaattctg ggatttaatt tcttttctta ccaaattcaa
1021 aattgcatta ggcttacctg accactacga agtatgatct ttactacctg tgaaaatcct
1081 tagttgatgg agaagattca cttgaattcc tcctcacttt tctttgggga cccattgcct
1141 cttcattaaa ccagggcaag ctttcacaga tgcagtaagt gaaaggtcac atagtatgta
1201 tatcaactaa cacagcattt taaaacaaaa tgaattaaaa tgatatcatt agtaattgat
1261 ctggtcatga gaggtgtgtg tgtttgtgtg tgtgtgtgtg tgtgtgtgtg agagagagag
1321 agagaaagag agagagagac agggaaatac attattcctc tggaaactct cacatggttt
1381 taattctttt ctctgggaac actgtccctc tgggaactct tacttggttc taccactcat
1441 tcccagagca gagtctattt aagacagaga catgtgcctt cagaacagaa tttattagaa
1501 actcactaaa atttgagagt ggtcacactt aggtcttgcc ataagtatca cctaatgaag
1561 agttatgcat agattaggac tgcagtagat gcattttaag tgaatcaagg aatttagaat
1621 aggaatgtat ctgcttattt cattccaatt atagtttgta taattgtaat ttcaatatca
1681 aattataaat taaaaatgtc ctcgtgggaa ctactgaaaa aaaaaaaaaa a
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