LOCUS BC063790 2731 bp mRNA linear HUM 02-JAN-2004
DEFINITION Homo sapiens hypothetical protein FLJ30058, mRNA (cDNA clone
MGC:70537 IMAGE:6196091), complete cds.
ACCESSION BC063790
VERSION BC063790.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2731)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 2731)
AUTHORS Strausberg,R.
TITLE Direct Submission
JOURNAL Submitted (08-DEC-2003) National Institutes of Health, Mammalian
Gene Collection (MGC), Cancer Genomics Office, National Cancer
Institute, 31 Center Drive, Room 11A03, Bethesda, MD 20892-2590,
USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: Dr. James R. Lupski
cDNA Library Preparation: Life Technologies, Inc.
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Genome Sequence Centre,
BC Cancer Agency, Vancouver, BC, Canada
info@bcgsc.bc.ca
Steven Jones, Jennifer Asano, Ian Bosdet, Yaron Butterfield,
Susanna Chan, Readman Chiu, Chris Fjell, Erin Garland, Ran Guin,
Letticia Hsiao, Martin Krzywinski, Reta Kutsche, Oliver Lee, Soo
Sen Lee, Victor Ling, Carrie Mathewson, Candice McLeavy, Steven
Ness, Pawan Pandoh, Anna-Liisa Prabhu, Parvaneh Saeedi, Jacqueline
Schein, Duane Smailus, Michael Smith, Lorraine Spence, Jeff Stott,
Michael Thorne, Miranada Tsai, Natasja van den Bosch, Jill Vardy,
George Yang, Scott Zuyderduyn, Marco Marra.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 141 Row: g Column: 1
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 21450648.
FEATURES Location/Qualifiers
source 1..2731
/db_xref="H-InvDB:HIT000261176"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:70537 IMAGE:6196091"
/tissue_type="Peripheral Nervous System, sympathetic
trunk"
/clone_lib="Lupski_sympathetic_trunk"
/lab_host="DH10B"
/note="Vector: pCMV-SPORT6"
gene 1..2731
/gene="FLJ30058"
/db_xref="GeneID:158763"
CDS 353..1588
/gene="FLJ30058"
/codon_start=1
/product="FLJ30058 protein"
/protein_id="AAH63790.1"
/db_xref="GeneID:158763"
/translation="MQVEEATGQAAGRRRGNVVRRVFGRIRRFFSRRRNEPTLPREFT
RRGRRGAVSVDSLAELEDGALLLQTLQLSKISFPIGQRLLGSKRKMSLNPIAKQIPQV
VEACCQFIEKHGLSAVGIFTLEYSVQRVRQLREEFDQGLDVVLDDNQNVHDVAALLKE
FFRDMKDSLLPDDLYMSFLLTATLKPQDQLSALQLLVYLMPPCHSDTLERLLKALHKI
TENCEDSIGIDGQLVPGNRMTSTNLALVFGSALLKKGKFGKRESRKTKLGIDHYVASV
NVVRAMIDNWDVLFQVPPHIQRQVAKRVWKSSPEALDFIRRRNLRKIQSARIKMEEDA
LLSDPVETSAEARAAVLAQSKPSDEGSSEEPAVPSGTARSHDDEEGAGNPPIPEQDRP
LLRVPREKEAKTGVSYFFP"
misc_feature 650..1111
/gene="FLJ30058"
/note="RhoGAP; Region: RhoGAP domain. GTPase activator
proteins towards Rho/Rac/Cdc42-like small GTPases"
/db_xref="CDD:pfam00620"
BASE COUNT 728 a 646 c 668 g 689 t
ORIGIN
1 attgtatgct actggcacct tgagaaggtt gaaagcaatg agtgtcttgg gaggagcccc
61 aggacacaac cccgaccgca ggacgaagat ggtatcgata cacagcctct ctgagctgga
121 gcgtctgaag ctgcaagaga ctgcttacca cgaactcgtg gccagacatt tcctctccga
181 attcaaacct gacagagctc tgcctattga ccgtccgaac accttggata agtggtttct
241 gattttgaga ggacagcaga gggctgtatc acacaagaca tttggcatta gcctggaaga
301 ggtcctggtg aacgagttta cccgccgcaa gcatcttgaa ctgacagcca cgatgcaggt
361 tgaagaagcc accggtcagg ctgcgggccg tcgtcgggga aacgtggtgc gaagggtgtt
421 tggccgcatc cggcgctttt tcagtcgcag gcggaatgag cccaccttgc cccgggagtt
481 cactcgccgt gggcgtcgag gtgcagtgtc tgtggatagt ctggctgagc tggaagacgg
541 agccctgctg ctgcagaccc tgcagctttc aaaaatttcc tttccaattg gccaacgact
601 tctgggatcc aaaaggaaga tgagtctcaa tccgattgcg aaacaaatcc cccaggttgt
661 tgaggcttgc tgccaattca ttgaaaaaca tggcttaagc gcagtgggga tttttaccct
721 tgaatactcc gtgcagcgag tgcgtcagct ccgtgaagaa tttgatcaag gtctggatgt
781 agtgctggat gacaatcaga atgtgcatga tgtggctgca ctcctcaagg agtttttccg
841 tgacatgaag gattctctgc tgccagatga tctgtacatg tcattcctcc tgacagcaac
901 tttaaagccc caggatcagc tttctgccct gcagttgctg gtctacctga tgccaccctg
961 ccacagtgat accctggagc gtctgctgaa ggccctgcat aaaatcactg agaactgcga
1021 ggactcaatt ggcattgatg gacagttggt cccaggcaac cgtatgactt ccactaactt
1081 ggccttggtg tttggatctg ctctcctgaa aaaaggaaag tttggcaaga gagagtccag
1141 gaaaacaaag ctggggattg atcactatgt tgcttctgtc aatgtggtcc gtgccatgat
1201 tgataactgg gatgtcctct tccaggtgcc tccccatatt cagaggcagg ttgctaagcg
1261 cgtgtggaag tccagcccgg aagcacttga ttttatcaga cgcaggaact tgaggaagat
1321 ccagagtgca cgcataaaga tggaagagga tgcactactt tctgatccag tggaaacctc
1381 tgctgaagcc cgggctgctg tccttgctca aagcaagcct tctgatgaag gttcctctga
1441 ggagccagct gtgccttccg gcactgcccg ttcccatgac gatgaggaag gagcgggtaa
1501 ccctcccatt ccggagcaag accgcccatt gctccgtgtg ccccgggaga aggaggccaa
1561 aactggcgtc agctacttct ttccttagat gtttttcctt ctataaggtg ccagacaggg
1621 gaaaagggtg ggggtacatc tgggatgtca caggaaacat taaggagaga gttgaaggta
1681 aagatctgaa ggtaagaagg agttccacct gatgctcggg tcaggatgag aattccaaac
1741 acactgccag ccccttcact ggggatgctt ggtctcttct gctggtaaaa gcagagatgt
1801 ttctgtgtca tgcccaagct ccccggtgct accttgcctt tctcttttac ccctgatctt
1861 ggctttctct ctctctctgc agactttcct ttaattgatg tgacatttgt ggtaaacacc
1921 tttcccaggg aacctcacaa atcttgagac gctttccctt ccccaaatgg gattgcatga
1981 tttccctgac tttcctaccc tcctccagag agctcagttg gaaaggccct caagaggcat
2041 gctagaacgt taggtcagcc tactgacagc tgacaaacaa ttaatgcgaa atcatgtcac
2101 accaacccat agccgtgtcc acgcagcaac tccaccacct taggatttcc ccctccaaat
2161 tattcagacc aatggcttgc caaatggcct ctcccaaaat tctgtacagt tttgctcagg
2221 tcacgccaac agggaaacct caagtgtagg tctaattagt gtttctggga tccaaagtta
2281 gaggaaaatt tagattttat tgcctggatc tgctttaaag acaattggtg tttacaccct
2341 cttgtcagca aaacagctag ttaggtaagg acatatagtt ccaagtaggt aaagtcactt
2401 gattacaaat gttcttaact atcgtctctg taattccttt atacaggaca gtacaaaatt
2461 gtgggacatg ctctggtaac acacagatat gggttgcata tgatccagaa ttacagctga
2521 tattatggat gacaactgct aaggtccata aaatgaagac tgtattgtat tgagggatag
2581 aaattgatta tttaatgggt aacaactgct gagctcaaag atttgtgatt gttaaaactt
2641 ctctggcatt taatcattaa taaacatctg tattgtgaca gcagcaaaaa aaaaaaaaaa
2701 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa a
//