LOCUS BC063784 2084 bp mRNA linear HUM 23-DEC-2003
DEFINITION Homo sapiens splicing factor 4, mRNA (cDNA clone IMAGE:6205595),
partial cds.
ACCESSION BC063784
VERSION BC063784.1
KEYWORDS .
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2084)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 2084)
AUTHORS Strausberg,R.
TITLE Direct Submission
JOURNAL Submitted (08-DEC-2003) National Institutes of Health, Mammalian
Gene Collection (MGC), Cancer Genomics Office, National Cancer
Institute, 31 Center Drive, Room 11A03, Bethesda, MD 20892-2590,
USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: Dr. James R. Lupski
cDNA Library Preparation: Life Technologies, Inc.
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Genome Sequence Centre,
BC Cancer Agency, Vancouver, BC, Canada
info@bcgsc.bc.ca
Steven Jones, Jennifer Asano, Ian Bosdet, Yaron Butterfield,
Susanna Chan, Readman Chiu, Chris Fjell, Erin Garland, Ran Guin,
Letticia Hsiao, Martin Krzywinski, Reta Kutsche, Oliver Lee, Soo
Sen Lee, Victor Ling, Carrie Mathewson, Candice McLeavy, Steven
Ness, Pawan Pandoh, Anna-Liisa Prabhu, Parvaneh Saeedi, Jacqueline
Schein, Duane Smailus, Michael Smith, Lorraine Spence, Jeff Stott,
Michael Thorne, Miranada Tsai, Natasja van den Bosch, Jill Vardy,
George Yang, Scott Zuyderduyn, Marco Marra.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 141 Row: h Column: 21
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 26665863.
FEATURES Location/Qualifiers
source 1..2084
/db_xref="H-InvDB:HIT000261170"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="IMAGE:6205595"
/tissue_type="Peripheral Nervous System, sciatic nerve"
/clone_lib="Lupski_sciatic_nerve"
/lab_host="DH10B"
/note="Vector: pCMV-SPORT6"
gene <1..2084
/gene="SF4"
/gene_synonym="DKFZp434E2216"
/gene_synonym="F23858"
/gene_synonym="RBP"
/db_xref="GeneID:57794"
/db_xref="MIM:607992"
CDS <1..1932
/gene="SF4"
/gene_synonym="DKFZp434E2216"
/gene_synonym="F23858"
/gene_synonym="RBP"
/codon_start=1
/product="SF4 protein"
/protein_id="AAH63784.1"
/db_xref="GeneID:57794"
/db_xref="MIM:607992"
/translation="LKMDNRDVAGKANRWFGVAPPKSGKMNMNILHQEELIAQKKREI
EAKMEQKAKQNQVASPQPPHPGEITNAHNSSCISNKFANDGSFLQQFLKLQKAQTSTD
APTSAPSAPPSTPTPSAGKRSLLISRRTGLGLASLPGPVKSYSHAKQLPVAHRPSVFQ
SPDEDEEEDYEQWLEIKVSPPEGAETRKVIEKLARFVAEGGPELEKVAMEDYKDNPAF
AFLHDKNSREFLYYRKKVAEIRKEAQKSQAASQKVSPPEDEEVKNLAEKLARFIADGG
PEVETIALQNNRENQAFSFLYEPNSQGYKYYRQKLEEFRKAKASSTGSFTAPDPGLKR
KSPPEALSGSLPPATTCPASSTPAPTIIPAPAAPGKPASAATVKRKRKSRWGPEEDKV
ELPPAELVQRDVDASPSPLSVQDLKGLGYEKGKPVGLVGVTELSDAQKKQLKEQQEMQ
QMYDMIMQHKRAMQDMQLLWEKAVQQHQHGYDSDEEVDSELGTWEHQLRRMEMDKTRE
WAEQLTKMGRGKHFIGDFLPPDELEKFMETFKALKEGREPDYSEYKEFKLTVENIGYQ
MLMKMGWKEGEGLGSEGQGIKNPVNKGTTTVDGAGFGIDRPAELSKEDDEYEAFRKRM
MLAYRFRPNPLNNPRRPYY"
misc_feature 559..723
/gene="SF4"
/gene_synonym="DKFZp434E2216"
/gene_synonym="F23858"
/gene_synonym="RBP"
/note="Surp; Region: Surp module. This domain is also
known as the SWAP domain. SWAP stands for
Suppressor-of-White-APricot. It has been suggested that
these domains may be RNA binding"
/db_xref="CDD:pfam01805"
misc_feature 784..948
/gene="SF4"
/gene_synonym="DKFZp434E2216"
/gene_synonym="F23858"
/gene_synonym="RBP"
/note="Surp; Region: Surp module. This domain is also
known as the SWAP domain. SWAP stands for
Suppressor-of-White-APricot. It has been suggested that
these domains may be RNA binding"
/db_xref="CDD:pfam01805"
misc_feature 1672..1815
/gene="SF4"
/gene_synonym="DKFZp434E2216"
/gene_synonym="F23858"
/gene_synonym="RBP"
/note="G_patch; Region: glycine rich nucleic binding
domain"
/db_xref="CDD:smart00443"
BASE COUNT 551 a 584 c 611 g 338 t
ORIGIN
1 ctcaagatgg acaaccggga tgttgcagga aaggctaacc ggtggtttgg ggttgctccc
61 cctaaatctg gaaaaatgaa catgaacatc cttcaccagg aagagctcat cgctcagaag
121 aaacgggaaa ttgaagccaa aatggaacag aaagccaagc agaatcaggt ggccagccct
181 cagcccccac atcctggcga aatcacaaat gcacacaact cttcctgcat ttccaacaag
241 tttgccaacg atggtagctt cttgcagcag tttctgaagt tgcagaaggc acagaccagc
301 acagacgccc cgaccagtgc gcccagcgcc cctcccagca cacccacccc cagcgctggg
361 aagaggtccc tgctcatcag caggcggaca ggcctggggc tggccagcct gccgggccct
421 gtgaagagct actcccacgc caagcagctg cccgtggcgc accgcccgag tgtcttccag
481 tcccctgacg aggacgagga ggaggactat gagcagtggc tggagatcaa agtttcaccc
541 ccagagggag ccgagactcg gaaagtgata gagaaattgg cccgctttgt ggcagaagga
601 ggccccgagt tagaaaaagt agctatggag gactacaagg ataacccagc atttgcattt
661 ttgcacgata agaatagcag ggaattcctc tactacagga agaaggtggc tgagataaga
721 aaggaagcac agaagtcgca ggcagcctct cagaaagttt cacccccaga ggacgaagag
781 gtcaagaacc ttgcagaaaa gttggccagg ttcatagcgg acgggggtcc cgaggtggaa
841 accattgccc tccagaacaa ccgtgagaac caggcattca gctttctgta tgagcccaat
901 agccaagggt acaagtacta ccgacagaag ctggaggagt tccggaaagc caaggccagc
961 tccacaggca gcttcacagc acctgatccc ggcctgaagc gcaagtcccc tcctgaggcc
1021 ctgtcagggt ccttaccccc agccaccacc tgccccgcct cgtccacgcc tgcgcccact
1081 atcatccctg ctccagctgc ccccgggaag ccagcctccg cagccaccgt gaagaggaag
1141 cggaagagcc ggtgggggcc tgaagaggat aaggtagagc tcccacctgc tgaactggtg
1201 cagagggacg tggatgcctc tccctcgcct ctgtcagttc aggacctcaa ggggctcggc
1261 tatgagaagg ggaagcctgt gggtctagtg ggcgtcacag agctttcaga cgcccagaag
1321 aagcagctga aggagcagca ggagatgcag cagatgtatg acatgatcat gcagcacaag
1381 cgggccatgc aggacatgca gctgctgtgg gagaaggcag tccaacagca ccagcacggc
1441 tatgacagtg atgaggaggt ggacagcgag ctgggcacct gggagcacca gctgcggcgc
1501 atggagatgg ataagaccag ggaatgggcc gagcagctga caaagatggg ccggggcaag
1561 cacttcatcg gagacttcct gcctccagac gagctggaaa agtttatgga gaccttcaag
1621 gccctgaagg agggccgtga gcctgactac tcagagtaca aggagttcaa gctgactgtg
1681 gagaacatcg gctaccagat gctgatgaag atgggctgga aggagggcga ggggctgggc
1741 tcagagggcc agggcatcaa gaacccagtg aacaagggca ccaccacagt ggacggcgct
1801 ggcttcggca ttgaccggcc ggcggagctc tccaaggagg acgacgagta tgaggcgttc
1861 cgcaagagga tgatgctggc ctaccgcttc cggcccaacc ccctgaacaa tcccagacgg
1921 ccttactact gagtgttctg gaaatacata ctttctgaat gaccaaccgt ccctggactg
1981 tggaatgttc cggcctgcat ttctgcccac cccttccgtt gtcacgagtg ccgtgccgtg
2041 taataaagtc ccagtgctca tccaccaaaa aaaaaaaaaa aaaa
//