LOCUS BC063706 989 bp mRNA linear HUM 03-JAN-2005
DEFINITION Homo sapiens spire homolog 2 (Drosophila), mRNA (cDNA clone
IMAGE:4803560), partial cds.
ACCESSION BC063706
VERSION BC063706.1
KEYWORDS .
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 989)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 989)
AUTHORS Director MGC Project.
TITLE Direct Submission
JOURNAL Submitted (08-DEC-2003) National Institutes of Health, Mammalian
Gene Collection (MGC), Cancer Genomics Office, National Cancer
Institute, 31 Center Drive, Room 11A03, Bethesda, MD 20892-2590,
USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: James Cleaver, M.D.
cDNA Library Preparation: Life Technologies, Inc.
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: National Institutes of Health Intramural
Sequencing Center (NISC),
Gaithersburg, Maryland;
Web site: http://www.nisc.nih.gov/
Contact: nisc_mgc@nhgri.nih.gov
Akhter,N., Ayele,K., Beckstrom-Sternberg,S.M., Benjamin,B.,
Blakesley,R.W., Bouffard,G.G., Breen,K., Brinkley,C., Brooks,S.,
Dietrich,N.L., Granite,S., Guan,X., Gupta,J., Haghighi,P.,
Hansen,N., Ho,S.-L., Karlins,E., Kwong,P., Laric,P., Legaspi,R.,
Maduro,Q.L., Masiello,C., Maskeri,B., Mastrian,S.D.,McCloskey,J.C.,
McDowell,J., Pearson,R., Stantripop,S., Thomas,P.J., Touchman,J.W.,
Tsurgeon,C., Vogt,J.L., Walker,M.A., Wetherby,K.D., Wiggins,L.,
Young,A., Zhang,L.-H. and Green,E.D.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 134 Row: n Column: 12
This clone was selected for full length sequencing because it
passed the following selection criteria: Hexamer frequency ORF
analysis.
FEATURES Location/Qualifiers
source 1..989
/db_xref="H-InvDB:HIT000261159"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="IMAGE:4803560"
/tissue_type="Skin, normal"
/clone_lib="NCI_CGAP_Skn3"
/lab_host="DH10B"
/note="Vector: pCMV-SPORT6.1"
gene <1..989
/gene="SPIRE2"
/gene_synonym="Spir-2"
/db_xref="GeneID:84501"
CDS <1..810
/gene="SPIRE2"
/gene_synonym="Spir-2"
/codon_start=1
/product="SPIRE2 protein"
/protein_id="AAH63706.1"
/db_xref="GeneID:84501"
/translation="LAQLRSEVASGLQSATHPPGGTEPPRPRAGSAHVWRPGSRDQGT
CPASVSDPSHPLLSNRGSSGDRPEASMTPDAKHLWLEFSHPVESLALTVEEVMDVRRV
LVKAEMEKFLQNKELFSSLKKGKICCCCRAKFPLFSWPPSCLFCKRAVCTSCSIKMKM
PSKKFGHIPVYTLGFESPQRVSAAKTAPIQRRDIFQSLQGPQWQSVEEAFPHIYSHGC
VLKDVCSECTSFVADVVRSSRKSVDVLNTTPRRSRQTQSLYIPNTRTLDFK"
BASE COUNT 255 a 286 c 263 g 185 t
ORIGIN
1 ctggcccagc tccgaagtga ggtggcctct ggcctgcagt cggccaccca ccccccagga
61 gggacggagc caccacggcc ccgagctggc agtgcgcatg tgtggaggcc cggctcccga
121 gaccagggta cctgtcccgc gagtgtctct gaccccagcc accccctact cagcaaccgg
181 ggctcctcgg gggacagacc cgaggcctcc atgacccccg atgccaaaca cctgtggctg
241 gagttcagcc accccgtgga gagcctggcg ctgactgtgg aagaggtgat ggacgtgcgc
301 cgtgtgctgg tgaaggccga gatggaaaag tttttgcaga acaaggagct cttcagcagt
361 ctgaagaagg ggaagatttg ctgctgctgc cgggccaagt tcccgctgtt ctcgtggccg
421 cccagctgtc tcttctgcaa gagagccgtc tgcacttcct gtagcataaa gatgaagatg
481 ccttctaaga aatttggaca catccctgtc tacacactgg gctttgagag tcctcagagg
541 gtatcagctg ccaaaaccgc gccaatccag agaagagaca tctttcagtc tctgcaaggg
601 ccacagtggc agagcgtgga ggaggcgttc ccccacatct actcccacgg ctgtgtcctg
661 aaggatgtct gcagtgagtg caccagcttt gtggcagacg tggtgcgttc cagccgcaag
721 agcgtggacg tcctcaacac tacgccacga cgcagtcgcc agacccaatc cctctacatc
781 cctaacacca ggactcttga cttcaagtga cagccccagg tggccaggcc tccaggaggc
841 accaggcagg ccctgtatca ggctaggacg ctctgagctg tgcatgtaca tatatacata
901 tatagataca tttataatat atacacacag tctatatatt tataaaaaaa aaaaaaaaaa
961 aaaaaaaaaa aaaaaaaaaa aaaaaaaaa
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