LOCUS BC063610 1914 bp mRNA linear HUM 15-JUL-2006
DEFINITION Homo sapiens tubulin, beta 2B, mRNA (cDNA clone MGC:70829
IMAGE:4941267), complete cds.
ACCESSION BC063610
VERSION BC063610.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1914)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 1914)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (08-DEC-2003) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: David N. Louis, M.D.
cDNA Library Preparation: Life Technologies, Inc.
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: National Institutes of Health Intramural
Sequencing Center (NISC),
Gaithersburg, Maryland;
Web site: http://www.nisc.nih.gov/
Contact: nisc_mgc@nhgri.nih.gov
Akhter,N., Ayele,K., Beckstrom-Sternberg,S.M., Benjamin,B.,
Blakesley,R.W., Bouffard,G.G., Breen,K., Brinkley,C., Brooks,S.,
Dietrich,N.L., Granite,S., Guan,X., Gupta,J., Haghighi,P.,
Hansen,N., Ho,S.-L., Karlins,E., Kwong,P., Laric,P., Legaspi,R.,
Maduro,Q.L., Masiello,C., Maskeri,B., Mastrian,S.D.,McCloskey,J.C.,
McDowell,J., Pearson,R., Stantripop,S., Thomas,P.J., Touchman,J.W.,
Tsurgeon,C., Vogt,J.L., Walker,M.A., Wetherby,K.D., Wiggins,L.,
Young,A., Zhang,L.-H. and Green,E.D.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 134 Row: o Column: 12
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 42476191.
FEATURES Location/Qualifiers
source 1..1914
/db_xref="H-InvDB:HIT000261064"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:70829 IMAGE:4941267"
/tissue_type="Brain, anaplastic oligodendroglioma with
1p/19q loss"
/clone_lib="NCI_CGAP_Brn67"
/lab_host="DH10B"
/note="Vector: pCMV-SPORT6"
gene 1..1914
/gene="TUBB2B"
/gene_synonym="bA506K6.1"
/gene_synonym="DKFZp566F223"
/gene_synonym="MGC8685"
/gene_synonym="RP11-506K6.1"
/gene_synonym="TUBB-PARALOG"
/db_xref="GeneID:347733"
/db_xref="HGNC:HGNC:30829"
CDS 95..1432
/gene="TUBB2B"
/gene_synonym="bA506K6.1"
/gene_synonym="DKFZp566F223"
/gene_synonym="MGC8685"
/gene_synonym="RP11-506K6.1"
/gene_synonym="TUBB-PARALOG"
/codon_start=1
/product="tubulin, beta 2B"
/protein_id="AAH63610.1"
/db_xref="GeneID:347733"
/db_xref="HGNC:HGNC:30829"
/translation="MREIVHIQAGQCGNQIGAKFWEVISDEHGIDPTGSYHGDSDLQL
ERINVYYNEATGNKYVPRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWA
KGHYTEGAELVDSVLDVVRKESESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYP
DRIMNTFSVMPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLT
TPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSR
GSQQYRALTVPELTQQMFDSKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQ
NKNSSYFVEWIPNNVKTAVCDIPPRGLKMSATFIGNSTAIQELFKRISEQFTAMFRRK
AFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQDATADEQGEFEEEEGEDEA"
BASE COUNT 458 a 517 c 551 g 388 t
ORIGIN
1 ccgagcgcct tcccggtgac cccgcagtgg gtgtgtgagg ggaggacgga cagacccaga
61 cgccgccgga ccaggaggac gctgacgagg caccatgcgt gagatcgtgc acatccaggc
121 gggccagtgc ggcaaccaga tcggcgccaa gttttgggag gtcatcagtg atgagcatgg
181 gattgacccc actggcagtt accatggaga cagtgatttg cagctggaga gaatcaatgt
241 ttactacaat gaagccactg gtaacaaata tgttcctcgg gccatcctcg tggatctgga
301 gccaggcacg atggattcgg ttaggtctgg accattcggc cagatcttca gaccagacaa
361 tttcgtgttt ggccagagtg gagccgggaa taactgggcc aagggccact acacagaggg
421 agccgagctg gtcgactcgg tcctggatgt ggtgaggaag gagtcagaga gctgtgactg
481 tctccagggc ttccagctga cccactctct ggggggcggc acggggtccg ggatgggcac
541 cctgctcatc agcaagatcc gggaagagta cccagaccgc atcatgaaca ccttcagcgt
601 catgccctca cccaaggtgt cagacacggt ggtggagccc tacaacgcca ccctctcggt
661 ccaccagctg gtggaaaaca cagatgaaac ctactgcatt gacaacgagg ccctgtatga
721 catctgcttc cgcaccctga agctgaccac ccccacctac ggggacctca accacctggt
781 gtcggccacc atgagcgggg tcaccacctg cctgcgcttc ccgggccagc tgaacgcaga
841 cctgcgcaag ctggcggtga acatggtgcc cttccctcgc ctgcacttct tcatgcccgg
901 cttcgcgccc ctgaccagcc ggggcagcca gcagtaccgg gcgctcacgg tgcccgagct
961 cacccagcag atgttcgact ccaagaacat gatggccgcc tgcgacccgc gccacggccg
1021 ctacctgacg gtggctgcca tcttccgggg ccgcatgtcc atgaaggagg tggacgagca
1081 gatgctcaac gtgcagaaca agaacagcag ctacttcgtg gagtggatcc ccaacaacgt
1141 gaagacggcc gtgtgcgaca tcccgccccg cggcctgaag atgtcggcca ccttcatcgg
1201 caacagcacg gccatccagg agctgttcaa gcgcatctcc gagcagttca cggccatgtt
1261 ccggcgcaag gccttcctgc actggtacac gggcgagggc atggacgaga tggagttcac
1321 cgaggccgag agcaacatga acgacctggt gtccgagtac cagcagtacc aggacgccac
1381 ggccgacgaa caaggggagt tcgaggagga ggagggcgag gacgaggcgt agatgccccc
1441 gcgagacggg ttagggaaag cggaggagga aagcgagggg gtggggggct tcccgggacg
1501 ataacctggc agtggaagga aagaagcatg gtctacttta ggtgtgcgct gggtctctgg
1561 tgctcttcac tgttgcctgt cacttttttt tccttttttg taatattgat gacatcaatg
1621 taacatttga gatatttctg aattactgtt gtaatggcta aaatcacata aacgtttgtg
1681 tcggaatggt gtcctctctt tctcttcctt tttctcttta ttaacgattt aaatgtaact
1741 ttctgaacac attgcattga attcttcctt taacaaaaag caaaggcgta ggtaaaagct
1801 caaatgaatt tattctttcg gtatggtaaa attgaaccaa tcacagttaa gatgagagat
1861 caacctgagt tttaaaatac ctttaataaa tattagttga aaaaaaaaaa aaaa
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