LOCUS BC063513 1797 bp mRNA linear HUM 19-MAR-2007
DEFINITION Homo sapiens keratin 8, mRNA (cDNA clone MGC:75150 IMAGE:3879823),
complete cds.
ACCESSION BC063513
VERSION BC063513.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1797)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 1797)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (08-DEC-2003) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: DCTD/DTP/Gazdar
cDNA Library Preparation: Life Technologies, Inc.
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: National Institutes of Health Intramural
Sequencing Center (NISC),
Gaithersburg, Maryland;
Web site: http://www.nisc.nih.gov/
Contact: nisc_mgc@nhgri.nih.gov
Akhter,N., Ayele,K., Beckstrom-Sternberg,S.M., Benjamin,B.,
Blakesley,R.W., Bouffard,G.G., Breen,K., Brinkley,C., Brooks,S.,
Dietrich,N.L., Granite,S., Guan,X., Gupta,J., Haghighi,P.,
Hansen,N., Ho,S.-L., Karlins,E., Kwong,P., Laric,P., Legaspi,R.,
Maduro,Q.L., Masiello,C., Maskeri,B., Mastrian,S.D.,McCloskey,J.C.,
McDowell,J., Pearson,R., Stantripop,S., Thomas,P.J., Touchman,J.W.,
Tsurgeon,C., Vogt,J.L., Walker,M.A., Wetherby,K.D., Wiggins,L.,
Young,A., Zhang,L.-H. and Green,E.D.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 134 Row: c Column: 19
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 68226427.
FEATURES Location/Qualifiers
source 1..1797
/db_xref="H-InvDB:HIT000260971"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:75150 IMAGE:3879823"
/tissue_type="Lung, large cell carcinoma"
/clone_lib="NIH_MGC_68"
/lab_host="DH10B"
/note="Vector: pCMV-SPORT6"
gene 1..1797
/gene="KRT8"
/gene_synonym="CARD2"
/gene_synonym="CK8"
/gene_synonym="CYK8"
/gene_synonym="K2C8"
/gene_synonym="K8"
/gene_synonym="KO"
/db_xref="GeneID:3856"
/db_xref="HGNC:HGNC:6446"
/db_xref="MIM:148060"
CDS 69..1520
/gene="KRT8"
/gene_synonym="CARD2"
/gene_synonym="CK8"
/gene_synonym="CYK8"
/gene_synonym="K2C8"
/gene_synonym="K8"
/gene_synonym="KO"
/codon_start=1
/product="keratin 8"
/protein_id="AAH63513.2"
/db_xref="GeneID:3856"
/db_xref="HGNC:HGNC:6446"
/db_xref="MIM:148060"
/translation="MSIRVTQKSYKVSTSGPRAFSSRSYTSGPGSRISSSSFSRVGSS
NFRGGLGGGYGGASGMGGITAVTVNQSLLSPLVLEVDPNIQAVRTQEKEQIKTLNNKF
ASFIDKVRFLEQQNKMLETKWSLLQQQKTARSNMDNMFESYINNLRRQLETLGQEKLK
LEAELGNMQGLVEDFKNKYEDEINKRTEMENEFVLIKKDVDEAYMNKVELESRLEGLT
DEINFLRQLYEEEIRELQSQISDTSVVLSMDNSRSLDMDSIIAEVKAQYEDIANRSRA
EAESMYQIKYEELQSLAGKHGDDLRRTKTEISEMNRNISRLQAEIEGLKGQRASLEAA
IADAEQRGELAIKDANAKLSELEAALQRAKQDMARQLREYQELMNVKLALDIEIATYR
KLLEGEESRLESGMQNMSIHTKTTSGYAGGLSSAYGGLTSPGLSYSLGSSFGSGAGSS
SFSRTSSSRAVVVKKIETRDGKLVSESSDVLPK"
BASE COUNT 442 a 513 c 534 g 308 t
ORIGIN
1 gcagcttctc cgctccttct aggatctccg cctggttcgg cccgcctgcc tccactcctg
61 cctccaccat gtccatcagg gtgacccaga agtcctacaa ggtgtccacc tctggccccc
121 gggccttcag cagccgctcc tacacgagtg ggcccggttc ccgcatcagc tcctcgagct
181 tctcccgagt gggcagcagc aactttcgcg gtggcctggg cggcggctat ggtggggcca
241 gcggcatggg aggcatcacc gcagttacgg tcaaccagag cctgctgagc ccccttgtcc
301 tggaggtgga ccccaacatc caggccgtgc gcacccagga gaaggagcag atcaagaccc
361 tcaacaacaa gtttgcctcc ttcatagaca aggtacggtt cctggagcag cagaacaaga
421 tgctggagac caagtggagc ctcctgcagc agcagaagac ggctcgaagc aacatggaca
481 acatgttcga gagctacatc aacaacctta ggcggcagct ggagactctg ggccaggaga
541 agctgaagct ggaggcggag cttggcaaca tgcaggggct ggtggaggac ttcaagaaca
601 agtatgagga tgagatcaat aagcgtacag agatggagaa cgaatttgtc ctcatcaaga
661 aggatgtgga tgaagcttac atgaacaagg tagagctgga gtctcgcctg gaagggctga
721 ccgacgagat caacttcctc aggcagctgt atgaagagga gatccgggag ctgcagtccc
781 agatctcgga cacatctgtg gtgctgtcca tggacaacag ccgctccctg gacatggaca
841 gcatcattgc tgaggtcaag gcacagtacg aggatattgc caaccgcagc cgggctgagg
901 ctgagagcat gtaccagatc aagtatgagg agctgcagag cctggctggg aagcacgggg
961 atgacctgcg gcgcacaaag actgagatct ctgagatgaa ccggaacatc agccggctcc
1021 aggctgagat tgagggcctc aaaggccaga gggcttccct ggaggccgcc attgcagatg
1081 ccgagcagcg tggagagctg gccattaagg atgccaacgc caagttgtcc gagctggagg
1141 ccgccctgca gcgggccaag caggacatgg cgcggcagct gcgtgagtac caggagctga
1201 tgaacgtcaa gctggccctg gacatcgaga tcgccaccta caggaagctg ctggagggcg
1261 aggagagccg gctggagtct gggatgcaga acatgagtat tcatacgaag accaccagcg
1321 gctatgcagg tggtctgagc tcggcctatg ggggcctcac aagccccggc ctcagctaca
1381 gcctgggctc cagctttggc tctggcgcgg gctccagctc cttcagccgc accagctcct
1441 ccagggccgt ggttgtgaag aagatcgaga cacgtgatgg gaagctggtg tctgagtcct
1501 ctgacgtcct gcccaagtga acagctgcgg cagcccctcc cagcctaccc ctcctgcgct
1561 gccccagagc ctgggaagga ggccgctatg cagggtagca ctgggaacag gagacccacc
1621 tgaggctcag ccctagccct cagcccacct ggggagttta ctacctgggg accccccttg
1681 cccatgcctc cagctacaaa acaattcaat tgcttttttt ttttggtcca aaataaaacc
1741 tcagctagct ctgccaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaa
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