LOCUS BC063501 4476 bp mRNA linear HUM 30-MAR-2005
DEFINITION Homo sapiens pleckstrin homology domain containing, family G (with
RhoGef domain) member 4, mRNA (cDNA clone MGC:75210 IMAGE:5527705),
complete cds.
ACCESSION BC063501
VERSION BC063501.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 4476)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 4476)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (08-DEC-2003) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC/DCTD/DTP
cDNA Library Preparation: Life Technologies, Inc.
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: National Institutes of Health Intramural
Sequencing Center (NISC),
Gaithersburg, Maryland;
Web site: http://www.nisc.nih.gov/
Contact: nisc_mgc@nhgri.nih.gov
Akhter,N., Ayele,K., Beckstrom-Sternberg,S.M., Benjamin,B.,
Blakesley,R.W., Bouffard,G.G., Breen,K., Brinkley,C., Brooks,S.,
Dietrich,N.L., Granite,S., Guan,X., Gupta,J., Haghighi,P.,
Hansen,N., Ho,S.-L., Karlins,E., Kwong,P., Laric,P., Legaspi,R.,
Maduro,Q.L., Masiello,C., Maskeri,B., Mastrian,S.D.,McCloskey,J.C.,
McDowell,J., Pearson,R., Stantripop,S., Thomas,P.J., Touchman,J.W.,
Tsurgeon,C., Vogt,J.L., Walker,M.A., Wetherby,K.D., Wiggins,L.,
Young,A., Zhang,L.-H. and Green,E.D.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 135 Row: p Column: 2.
FEATURES Location/Qualifiers
source 1..4476
/db_xref="H-InvDB:HIT000260960"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:75210 IMAGE:5527705"
/tissue_type="Skin, melanotic melanoma."
/clone_lib="NIH_MGC_72"
/lab_host="DH10B"
/note="Vector: pCMV-SPORT6"
gene 1..4476
/gene="PLEKHG4"
/gene_synonym="DKFZP434I216"
/db_xref="GeneID:25894"
CDS 211..3786
/gene="PLEKHG4"
/gene_synonym="DKFZP434I216"
/codon_start=1
/product="PLEKHG4 protein"
/protein_id="AAH63501.1"
/db_xref="GeneID:25894"
/translation="MERPLENGDESPDSQGHATDWRFAVCSFRDAWEEEEPASQMHVK
DPGPPRPPAGATQDEELQGSPLSRKFQLPPAADESGDAQRGTVESSSVLSEGPGPSGV
ESLLCPMSSHLSLAQGESDTPGVGLVGDPGPSRAMPSGLSPGALDSDPVGLGDPLSEI
SKLLEAAPSGSGLPKPADCLLAQDLCWELLASGMATLPGTRDVQGRAVLLLCAHSPAW
LQSECSSQELIRLLLYLRSIPRPEVQALGLTVLVDARICAPSSSLFSGLSQLQEAAPG
AVYQVLLVGSTLLKEVPSGLQLEQLPSQSLLTHIPTAGLPTSLGGGLPYCHQAWLDFR
RRLEALLQNCQAACALLQGAIESVKAVPQPMEPGEVGQLLQQTEVLMQQVLDSPWLAW
LQCQGGRELTWLKQEVPEVTLSPDYRTAMDKADELYDRVDGLLHQLTLQSNQRIQALE
LVQTLEARESGLHQIEVWLQQVGWPALEEAGEPSLDMLLQAQGSFQELYQVAQEQVRQ
GEKFLQPLTGWEAAELDPPGARFLALRAQLTEFSRALAQRCQRLADAERLFQLFREAL
TWAEEGQRVLAELEQERPGVVLQQLQLHWTRHPDLPPAHFRKMWALATGLGSEAIRQE
CRWAWARCQDTWLALDQKLEASLKLPPVGSTASLCVSQVPAAPAHPPLRKAYSFDRNL
GQSLSEPACHCHHAATIAACRRPEAGGGALPQASPTVPPPGSSDPRSLNRLQLVLAEM
VATEREYVRALEYTMENYFPELDRPDVPQGLRGQRAHLFGNLEKLRDFHCHFFLRELE
ACTRHPPRVAYAFLRHRVQFGMYALYSKNKPRSDALMSSYGHTFFKDKQQALGDHLDL
ASYLLKPIQRMGKYALLLQELARACGGPTQELSALREAQSLVHFQLRHGNDLLAMDAI
QGCDVNLKEQGQLVRQDEFVVRTGRHKSVRRIFLFEELLLFSKPRHGPTGVDTFAYKR
SFKMADLGLTECCGNSNLRFEIWFRRRKARDTFVLQASSLAIKQAWTADISHLLWRQA
VHNKEVRMAEMVSMGVGNKAFRDIAPSEEAINDRTVNYVLKCREVRSRASIAVAPFDH
DSLYLGASNSLPGDPASCSVLGSLNLHLYRDPALLGLRCPLYPSFPEEAALEAEAELG
GQPSLTAEDSEISSQCPSASGSSGSDSSCVSGQALGRGLEDLPCV"
BASE COUNT 872 a 1355 c 1358 g 891 t
ORIGIN
1 gctgtccgac ttcacggttc cccgcggctc ggcccggggg cgcctgaatt gcagttcctg
61 tgccctggca ctaagactgg cacctcctgc ggcccatgcc cttcgcctgc attgcccact
121 gagtcccact cgactgtctt cagcgcggtt cacactgaga cccagccctc tcccgctggc
181 cccgagcagg cccaccttta ggagggcgtg atggaaaggc ccctggagaa tggggatgag
241 tccccagact ctcagggcca tgccaccgac tggagatttg ctgtgtgcag tttcagggat
301 gcctgggaag aggaggaacc tgcttcccag atgcacgtta aggacccagg tcctccaaga
361 ccaccagccg gggccaccca ggatgaggag ctacagggca gccccttgtc caggaaattc
421 cagttacccc cagctgcaga tgagtcgggg gatgcccaga ggggcacagt agaaagctcc
481 tcagtcctgt cagaagggcc aggcccctct ggagtggaga gtctcctatg ccccatgtcc
541 tcccacctca gcttggcaca gggtgagagt gacaccccag gggtagggtt ggtaggggac
601 ccaggtccaa gcagggcgat gccatctggc ttgagccctg gggcattgga cagcgaccct
661 gtgggccttg gagacccttt atcagaaata tcaaagctgc tggaggcagc ccccagtgga
721 tccggcctcc ctaagcctgc tgactgcctc ctggcccaag acctctgttg ggagctgctg
781 gccagtggta tggccacctt gccagggact cgggatgtcc aaggccgggc agtgctgctt
841 ctgtgtgccc acagcccagc ctggcttcag tctgagtgca gcagccagga actcatccgc
901 ctcctgctgt acctgcgaag catccccagg cccgaagtac aggcactggg actgacagtg
961 ctagttgatg cccgaatttg tgctccaagt tcttccctct tctctgggct cagccaacta
1021 caagaagcag ccccaggggc cgtgtaccag gtgctgctag tgggaagcac gctgctgaag
1081 gaagtgcctt ccgggctgca gctggagcag ttgccttctc agagcctgct gacccacatc
1141 ccaacggcgg ggctgcccac ttcgctagga ggaggcctgc cttactgcca ccaggcctgg
1201 ctggatttcc gaaggcggct ggaagctcta ctacagaact gccaggcagc ttgtgccctg
1261 ctccaggggg ccatcgaaag tgtgaaggct gtgccccagc ccatggagcc tggggaggtc
1321 ggtcagctgc tacagcagac agaggtcctg atgcagcagg tgctagactc gccatggctg
1381 gcatggctac aatgccaggg gggccgggag ctgacatggc tgaagcaaga ggtcccagag
1441 gtgaccctga gcccagacta caggacggca atggacaagg ctgacgagct atatgaccgg
1501 gtggatggat tgctgcacca actgaccctg cagagcaacc agcgaataca ggccctagag
1561 ttggtccaaa cactggaggc ccgggaaagc ggactgcacc agattgaagt gtggctgcag
1621 caggtgggct ggccagcact ggaggaggct ggggagccct cgctggacat gctgctccag
1681 gcccaaggct cttttcagga gctgtaccag gttgcccagg agcaggtcag gcaaggggag
1741 aagtttctgc agccgctgac tggctgggag gcggctgaac tggacccccc tggggcacgc
1801 tttctggccc tgcgagccca gctgactgaa ttctctaggg ctttggccca gcggtgccag
1861 cggctggcgg atgctgagag gctgtttcag ctcttcaggg aggccttgac gtgggctgag
1921 gaggggcagc gagtgttggc agagctggag caggaacgcc cgggggttgt gttgcagcag
1981 ctgcagctgc actggaccag gcaccctgac ttgcctcctg cccacttccg aaagatgtgg
2041 gctctggcca cggggctggg ctcagaggcc atccgccagg agtgccgctg ggcctgggcg
2101 cggtgccagg acacctggct ggccctggac caaaagcttg aggcttcact gaagctacca
2161 ccggtgggca gcacagctag cctgtgtgtc agccaggtcc ccgctgcacc tgcccaccct
2221 cccctgagga aggcctacag cttcgatcgg aatctggggc agagtctcag tgaacctgcc
2281 tgccactgcc accatgcggc cactattgct gcctgccgca gaccagaggc tggaggaggt
2341 gccctgcccc aggcatcccc tactgtgcct ccaccaggca gctctgaccc caggagcctc
2401 aacaggctac agctggtgct ggcagagatg gtggccacgg agcgggagta tgtccgggct
2461 ctagagtaca ctatggagaa ctatttcccc gagctggatc gccccgatgt gccccagggc
2521 ctccgcggtc agcgtgccca cctctttggc aacctggaga agctgcggga cttccactgc
2581 cacttcttcc tgcgtgagct ggaggcttgc acccggcacc caccacgagt ggcctatgcc
2641 ttcctgcgcc atagggtgca gtttgggatg tacgcgctct acagcaagaa taagcctcgc
2701 tccgatgccc tgatgtcaag ctatgggcac accttcttca aggacaagca gcaagcactg
2761 ggggaccacc tggacctggc ctcctacctg ctaaagccca tccagcgcat gggcaagtac
2821 gcactgctgc tgcaggagct ggcacgggcc tgcgggggcc ccacgcagga gctcagtgcg
2881 ctgcgggagg cccagagcct tgtgcacttc cagctgcggc acggaaacga cctgctggcc
2941 atggacgcca tccagggctg tgatgttaac ctcaaggaac aggggcagct ggtgcgacag
3001 gatgagtttg tggtgcgcac tgggcgccac aagtccgtgc gccgcatctt cctttttgag
3061 gagctgctgc tcttcagcaa gcctcgccat gggcccacag gggttgacac atttgcctac
3121 aagcgctcct tcaagatggc agaccttggt ctcactgagt gctgtgggaa cagcaacctg
3181 cgcttcgaga tctggttccg ccgccgcaag gccagggaca cctttgtgct gcaggcctcc
3241 agcctggcta tcaagcaggc ctggacagct gacatctccc acctgctttg gaggcaggcc
3301 gtccacaaca aggaggtgcg catggctgag atggtgtcca tgggtgtggg gaacaaggcc
3361 ttccgagaca ttgctcccag cgaggaagcc atcaacgacc gcaccgtcaa ctatgtcctg
3421 aagtgccgag aagttcgctc tcgggcgtcc attgccgtag ccccgtttga ccatgacagc
3481 ctctacctgg gggcctcgaa ctcccttcct ggagaccctg cctcttgctc tgttctgggg
3541 tccctcaacc tgcacctgta cagagaccca gctcttctgg gtctccgctg tcccctgtat
3601 cccagcttcc cagaggaagc agcactggag gctgaggcag agctgggcgg ccagccctct
3661 ttgactgctg aggactcaga gatctcgtcc caatgcccat cagccagtgg ctccagtggc
3721 tctgacagca gctgtgtgtc agggcaggcc ctgggtaggg gcctggagga cttaccctgt
3781 gtctgagccc gggactggac gagcagtaga tccagcagcc tgcagctcca aggaacattg
3841 cctctctgga tctgctgtga ccagggtgtg gctgacacct gggctacctc caacctacat
3901 gtgcaacgct gttgactacc ctttctgatg tgtgtggcca ttggactaac tggcacgggg
3961 cctctctagg gaagtctggt tgtagagcct gaataggctc ctggccccat gaccccttct
4021 cctgtcccca gctcccatcc cagttgtggg ttaagaatag gctagagcag acattgggtg
4081 tttccatgct gtaggctggt gggggaccat gtgcctctag gcagtgacta gggtgccccc
4141 acccctcagg aagaacacag gtgggctcct agcagctgat ccccaatgcc tggccttaaa
4201 gccgagctca gttaccatag ggacaggtcc acctctactg ggccctcatg cttgcctttc
4261 ctggccccca ggcccagccc ctttttactg gggcagtttc gttattttga cttgatccct
4321 tttgaataac tttcaataga attgtctaaa attatcttac tggttgttag gcctttggtg
4381 tctcagagaa ggagtctagg tctttgatgt gtgatttaat cttttatttg tttataataa
4441 aaaatagact gataaaaaaa aaaaaaaaaa aaaaaa
//