LOCUS BC063436 1745 bp mRNA linear HUM 23-DEC-2003
DEFINITION Homo sapiens double C2-like domains, alpha, mRNA (cDNA clone
MGC:70835 IMAGE:5394616), complete cds.
ACCESSION BC063436
VERSION BC063436.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1745)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 1745)
AUTHORS Strausberg,R.
TITLE Direct Submission
JOURNAL Submitted (08-DEC-2003) National Institutes of Health, Mammalian
Gene Collection (MGC), Cancer Genomics Office, National Cancer
Institute, 31 Center Drive, Room 11A03, Bethesda, MD 20892-2590,
USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: DCTD/DTP
cDNA Library Preparation: Life Technologies, Inc.
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: National Institutes of Health Intramural
Sequencing Center (NISC),
Gaithersburg, Maryland;
Web site: http://www.nisc.nih.gov/
Contact: nisc_mgc@nhgri.nih.gov
Akhter,N., Ayele,K., Beckstrom-Sternberg,S.M., Benjamin,B.,
Blakesley,R.W., Bouffard,G.G., Breen,K., Brinkley,C., Brooks,S.,
Dietrich,N.L., Granite,S., Guan,X., Gupta,J., Haghighi,P.,
Hansen,N., Ho,S.-L., Karlins,E., Kwong,P., Laric,P., Legaspi,R.,
Maduro,Q.L., Masiello,C., Maskeri,B., Mastrian,S.D.,McCloskey,J.C.,
McDowell,J., Pearson,R., Stantripop,S., Thomas,P.J., Touchman,J.W.,
Tsurgeon,C., Vogt,J.L., Walker,M.A., Wetherby,K.D., Wiggins,L.,
Young,A., Zhang,L.-H. and Green,E.D.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 135 Row: n Column: 17
This clone was selected for full length sequencing because it
passed the following selection criteria: GenomeScan gene
prediction, Similarity but not identity to protein.
FEATURES Location/Qualifiers
source 1..1745
/db_xref="H-InvDB:HIT000260896"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:70835 IMAGE:5394616"
/tissue_type="Breast, mammary adenocarcinoma."
/clone_lib="NIH_MGC_87"
/lab_host="DH10B"
/note="Vector: pCMV-SPORT6"
gene 1..1745
/gene="DOC2A"
/db_xref="GeneID:8448"
/db_xref="MIM:604567"
CDS 157..1359
/gene="DOC2A"
/codon_start=1
/product="DOC2A protein"
/protein_id="AAH63436.1"
/db_xref="GeneID:8448"
/db_xref="MIM:604567"
/translation="MRGRRGDRMTINIQEHMAINVCPGPIRPIRQISDYFPRGPGPEG
GGGSGGEAPAHLVPLALAPPAALLGATTPEDGAEVDSYDSDDATALGTLEFDLLYDRA
SCTLHCSILRAKGLKPMDFNGLADPYVKLHLLPGACKANKLKTKTQRNTLNPVWNEDL
TYSGITDDDITHKVLRIAVCDEDKLSHNEFIGEIRVPLRRLKPSQKKHFNICLERQVP
LASPSSMSAALRGISCYLKELEQAEQGQGLLEERGRILLSLSYSSRRRGLLVGILRCA
HLAAMDVNGYSDPYVKTYLRPDVDKKSKHKTCVKKKTLNPEFNEEFFYEIELSTLATK
TLEVTVWDYDIGKSNDFIGGVSLGPGARGEARKHWSDCLQQPDAALERWHTLTSELPP
AAGALSSA"
misc_feature 424..771
/gene="DOC2A"
/note="C2_1; Region: Protein kinase C conserved region 2,
subgroup 1"
/db_xref="CDD:cd00276"
misc_feature 964..1317
/gene="DOC2A"
/note="C2_1; Region: Protein kinase C conserved region 2,
subgroup 1"
/db_xref="CDD:cd00276"
BASE COUNT 355 a 569 c 505 g 316 t
ORIGIN
1 aagagatcct cccaccatca cgaggtcagg agatcgagac catcttggct aagaagctcg
61 actccgcgct ctcctcgccg gccgccgccg cccagcctgc gcccgccgtc cccccagtgc
121 ctgggcacct gttggaggca gagccagggg tgctgcatga ggggccgcag gggcgatcgc
181 atgaccatca acatccagga gcacatggcc atcaacgtgt gccccgggcc catccggccc
241 atccgccaga tctctgacta cttcccccgg ggaccaggac ctgaaggggg cggcgggagc
301 ggcggggagg cccccgccca tctggtcccc ctggctctgg ccccccctgc agccctcctt
361 ggggccacca cgcctgagga tggtgcggag gtggacagct atgactcgga tgatgccacc
421 gccctaggca cgctggagtt tgaccttctc tacgaccggg cctcctgcac tctgcactgt
481 agcatcctca gggccaaggg cctcaagccc atggatttca atggcctcgc cgacccctac
541 gtcaagctgc acttgctgcc tggagcctgt aaggccaata agctaaaaac gaagactcag
601 aggaacacac tgaatcccgt gtggaatgag gacctgactt acagcgggat cacagatgac
661 gacatcacgc acaaggtgct caggatcgcc gtctgtgatg aggacaagct gagtcacaat
721 gagtttattg gggagatccg cgtgcccctc cgccgcctca agccttcgca gaagaagcat
781 tttaacatct gcctcgagcg ccaagtcccg ctggcgtccc cctcttccat gtcagcggcg
841 ctgaggggca tctcctgtta tctgaaggag ttggagcagg cggagcaggg gcaggggctg
901 ctggaggagc gtggccgcat cctgctgagt ctcagctaca gctcgcggcg ccggggactg
961 ctggtaggca tcttgcgctg cgcccatctg gctgccatgg acgtcaacgg ttactcggac
1021 ccctacgtca agacgtacct gaggcccgat gtggacaaga aatccaagca taagacgtgt
1081 gtgaagaaga agactctcaa cccagaattt aacgaggagt ttttctacga gatagagctc
1141 tccactctgg ccaccaagac cctggaagtc accgtctggg actatgacat tggcaaatcc
1201 aatgacttca ttggtggcgt gtccctgggg ccaggtgccc gaggcgaggc tcggaagcac
1261 tggagtgact gcctgcagca gccggacgca gccctggagc gctggcacac cctgaccagt
1321 gagctgcccc ctgcggccgg ggctctgtcc tcagcctgag tggacagcag tgtcccggca
1381 caggcccatc gagccgggtc cagtacccaa ccttcgcacg agtgtgttgc acgtttacac
1441 agggtgggct gcccccaccc tgcactacct atttttgtga gtctccgtga cccgggtctg
1501 tctgctcatg aggggctgcg gagttctata ttcacatatg caaacctcct gcctgactcg
1561 ctagtccctg caaatatgca aaccccccat catctgcaca cccgggcagt gctcagagcc
1621 cggccccagg cccccgcgct cctcactcct gcctctccac gctgccccgt ccctctcccc
1681 caacagggag gaggtcggat taggggaggt tcagaggagg agaatgtctc aaaaaaaaaa
1741 aaaaa
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