LOCUS BC063379 1831 bp mRNA linear HUM 15-FEB-2005
DEFINITION Homo sapiens dihydrofolate reductase-like 1, mRNA (cDNA clone
MGC:71388 IMAGE:4109721), complete cds.
ACCESSION BC063379
VERSION BC063379.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1831)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 1831)
AUTHORS Director MGC Project.
TITLE Direct Submission
JOURNAL Submitted (08-DEC-2003) National Institutes of Health, Mammalian
Gene Collection (MGC), Cancer Genomics Office, National Cancer
Institute, 31 Center Drive, Room 11A03, Bethesda, MD 20892-2590,
USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC
cDNA Library Preparation: Rubin Laboratory
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: National Institutes of Health Intramural
Sequencing Center (NISC),
Gaithersburg, Maryland;
Web site: http://www.nisc.nih.gov/
Contact: nisc_mgc@nhgri.nih.gov
Akhter,N., Ayele,K., Beckstrom-Sternberg,S.M., Benjamin,B.,
Blakesley,R.W., Bouffard,G.G., Breen,K., Brinkley,C., Brooks,S.,
Dietrich,N.L., Granite,S., Guan,X., Gupta,J., Haghighi,P.,
Hansen,N., Ho,S.-L., Karlins,E., Kwong,P., Laric,P., Legaspi,R.,
Maduro,Q.L., Masiello,C., Maskeri,B., Mastrian,S.D.,McCloskey,J.C.,
McDowell,J., Pearson,R., Stantripop,S., Thomas,P.J., Touchman,J.W.,
Tsurgeon,C., Vogt,J.L., Walker,M.A., Wetherby,K.D., Wiggins,L.,
Young,A., Zhang,L.-H. and Green,E.D.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 50 Row: c Column: 7
This clone was selected for full length sequencing because it
passed the following selection criteria: Hexamer frequency ORF
analysis.
FEATURES Location/Qualifiers
source 1..1831
/db_xref="H-InvDB:HIT000260841"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:71388 IMAGE:4109721"
/tissue_type="Muscle, rhabdomyosarcoma"
/clone_lib="NIH_MGC_17"
/lab_host="DH10B-R"
/note="Vector: pOTB7"
gene 1..1831
/gene="DHFRL1"
/gene_synonym="DHFRP4"
/db_xref="GeneID:200895"
CDS 127..690
/gene="DHFRL1"
/gene_synonym="DHFRP4"
/codon_start=1
/product="DHFRL1 protein"
/protein_id="AAH63379.1"
/db_xref="GeneID:200895"
/translation="MFLLLNCIVAVSQNMGIGKNGDLPRPPLRNEFRYFQRMTTTSSV
EGKQNLVIMGRKTWFSIPEKNRPLKDRINLVLSRELKEPPQGAHFLARSLDDALKLTE
RPELANKVDMIWIVGGSSVYKEAMNHLGHLKLFVTRIMQDFESDTFFSEIDLEKYKLL
PEYPGILSDVQEGKHIKYKFEVCEKDD"
BASE COUNT 563 a 362 c 404 g 502 t
ORIGIN
1 ggtaaaggct gatacttccc aggacgcgga ggcacgtaaa aaaatttgaa gaagggaatt
61 tcgcggcatt cttggcctgg cttcctggcg tagccagcaa gttcggaggt gttaaccgct
121 gctgtcatgt ttcttttgct aaactgcatc gtcgctgtgt cccaaaacat gggcatcggc
181 aagaacgggg acctgcccag gccgccgctc aggaatgaat tcaggtattt ccagagaatg
241 accacaactt cttcagtaga gggtaaacag aatctggtga ttatgggtag gaagacctgg
301 ttctccattc ctgagaagaa tcgaccttta aaggatagaa ttaatttagt tctcagcaga
361 gaactcaagg aacctccaca aggagctcat tttcttgcca gaagtttgga tgatgcctta
421 aaacttactg aacgaccaga attagcaaat aaagtagaca tgatttggat agttggtggc
481 agttctgttt ataaggaagc catgaatcac ctaggccatc ttaaactatt tgtgacaagg
541 atcatgcagg actttgaaag tgacacgttt ttttcagaaa ttgacttgga gaaatataaa
601 cttctgcctg aatacccagg tattctctct gatgtccagg aggggaaaca catcaagtac
661 aaatttgaag tatgtgagaa ggatgattaa tatgaaggtg ttttctggtt taagttgttc
721 cccctccctc tgagaaaagt atgcattttt acattagaaa agggactttt gttgacttca
781 gatctatgga taattatttc taagcaacgt gtttttattc ctcactaatc ttggctatat
841 cagataccat ttatgaaaca ttcttgctat aactgtctct ccaagacccc gactgagtcc
901 ccagcacctg ctacagtgag ctgccattcc acacccatca catgtggcac tcttgccact
961 ccttgacatt gtcaggcttt tctaatgttg gtagtattta ttaaagatga agatgcacat
1021 acccttcagc tgagcagttt cactagtagg aaataccaaa agcttcgtac atgtatatcc
1081 agaggtttgt agacaaatgt tgcagccttt tttgtaacag tgaaaaactg aaaacaacct
1141 ggaagtccag tgatgggaaa atgaatatat ttctgtctta gattggggaa cccaaagcag
1201 attccaagac tgaaatttaa gtgaaagcag tttatttgct aggtcatacc agaagtcatc
1261 aatcgaagta tggagaaatg gaactgagaa ggtaaaaacc agttcaaaat cagtgaggag
1321 gttctcaatg gtaacgagct ccatactgct gagatacagg gaaacggagg ggagaaagct
1381 agagtattta acccccactc cttggttgtc agctccctgt cctgtgtgtg ggcagaacgt
1441 actccaccta ctcaatagca agttccaggt gtttgctgaa agaagctgct gtaatgtacg
1501 ggaacggtga atgccaaaca cttaaagcaa ttccatgttt aagtatgtaa actcttcata
1561 cttttttttt tttttctttt tgagacagaa tttcactctt gttgcccagg cggagtgcaa
1621 tggaacaacc ttggctcacg gcaacttcag ccttcccagc tactcaggag gctaagcaaa
1681 aggatggctt gagcccaaga gttcagagca gcctggccac catagtgaga tcccttctct
1741 taaaaataat aggttattgc cagatttggg gcatttggaa agaagtgcat tgaagataaa
1801 gcaaaaaaaa aaaaaaaaaa aaaaaaaaaa a
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