LOCUS BC063137 2291 bp mRNA linear HUM 15-JUL-2006
DEFINITION Homo sapiens pyruvate dehydrogenase kinase, isozyme 2, mRNA (cDNA
clone MGC:72153 IMAGE:4272593), complete cds.
ACCESSION BC063137
VERSION BC063137.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2291)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 2291)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (02-DEC-2003) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: CLONTECH
cDNA Library Preparation: CLONTECH Laboratories, Inc.
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Genome Sequence Centre,
BC Cancer Agency, Vancouver, BC, Canada
info@bcgsc.bc.ca
Martin Hirst, Thomas Zeng, Ryan Morin, Michelle Moksa, Johnson
Pang, Diana Mah, Jing Wang, Kieth Fichter, Eric Chuah, Allen
Delaney, Rob Kirkpatrick, Agnes Baross, Sarah Barber, Mabel
Brown-John, Steve S. Chand, William Chow, Ryan Babakaiff, Dave
Wong, Corey Matsuo, Jaclyn Beland, Susan Gibson, Luis delRio, Ruth
Featherstone, Malachi Griffith, Obi Griffith, Ran Guin, Nancy Liao,
Kim MacDonald, Mike R. Mayo, Josh Moran, Diana Palmquist, JR
Santos, Duane Smailus, Jeff Stott, Miranda Tsai, George Yang,
Jacquie Schein, Asim Siddiqui,Steven Jones, Rob Holt, Marco Marra.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 51 Row: f Column: 21
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 31543388.
FEATURES Location/Qualifiers
source 1..2291
/db_xref="H-InvDB:HIT000260784"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:72153 IMAGE:4272593"
/tissue_type="Prostate"
/clone_lib="NIH_MGC_83"
/lab_host="DH10B"
/note="Vector: pDNR-LIB"
gene 1..2291
/gene="PDK2"
/db_xref="GeneID:5164"
/db_xref="HGNC:HGNC:8810"
/db_xref="MIM:602525"
CDS 76..1299
/gene="PDK2"
/codon_start=1
/product="pyruvate dehydrogenase kinase, isozyme 2"
/protein_id="AAH63137.1"
/db_xref="GeneID:5164"
/db_xref="HGNC:HGNC:8810"
/db_xref="MIM:602525"
/translation="MRWVWALLKNASLAGAPKYIEHFSKFSPSPLSMKQFLDFGSSNA
CEKTSFTFLRQELPVRLANIMKEINLLPDRVLSTPSVQLVQSWYVQSLLDIMEFLDKD
PEDHRTLSQFTDALVTIRNRHNDVVPTMAQGVLEYKDTYGDDPVSNQNIQYFLDRFYL
SRISIRMLINQHTLIFDGSTNPAHPKHIGSIDPNCNVSEVVKDAYDMAKLLCDKYYMA
SPDLEIQEINAANSKQPIHMVYVPSHLYHMLFELFKNAMRATVESHESSLILPPIKVM
VALGEEDLSIKMSDRGGGVPLRKIERLFSYMYSTAPTPQPGTGGTPLAGFGYGLPISR
LYAKYFQRDLQLFSMEGFGTDAVIYLKALSTDSVERLPVYNKSAWRHYQTIQEAGDWC
VPSTEPKNTSTYRVS"
BASE COUNT 483 a 744 c 606 g 458 t
ORIGIN
1 gggggtcgct gggccgaaag gtgcgcgagc gctgcccgcg cggggaccac aacccaagtc
61 gcggccgccg cagccatgcg ctgggtgtgg gcgctgctga agaatgcgtc cctggcaggg
121 gcgcccaagt acatagagca cttcagcaag ttctccccgt ccccgctgtc catgaagcag
181 tttctggact tcggatccag caatgcctgt gagaaaacct ccttcacctt cctcaggcag
241 gagctgcctg tgcgcctggc caacatcatg aaagagatca acctgcttcc cgaccgagtg
301 ctgagcacac cctctgtgca gctggtgcag agctggtatg tccagagcct cctggacatc
361 atggagttcc tggacaagga tcccgaggac catcgcaccc tgagccagtt cactgacgcc
421 ctggtcacca tccggaaccg gcacaacgac gtggtgccca ccatggcaca aggcgtgctt
481 gagtacaagg acacctacgg cgatgacccc gtctccaacc agaacatcca gtacttcctg
541 gaccgcttct acctcagccg catctccatc cgcatgctca tcaaccagca caccctcatc
601 tttgatggca gcaccaaccc agcccatccc aaacacatcg gcagcatcga ccccaactgc
661 aacgtctctg aggtggtcaa agatgcctac gacatggcta agctcctgtg tgacaagtat
721 tacatggcct cacctgacct ggagatccag gagatcaatg cagccaactc caaacagccg
781 attcacatgg tctacgtccc ctcccacctc taccacatgc tctttgagct cttcaagaat
841 gccatgaggg cgactgtgga aagccatgag tccagcctca ttctcccacc catcaaggtc
901 atggtggcct tgggtgagga agatctgtcc atcaagatga gtgaccgagg tgggggtgtt
961 cccttgagga agattgagcg actcttcagc tacatgtact ccacagcacc caccccccag
1021 cctggcaccg ggggaacgcc gctggctggc tttggttatg ggctccccat ttcccgcctc
1081 tacgccaagt acttccagag agacctgcag ctcttctcca tggaaggctt tgggaccgat
1141 gctgtcatct atctcaaggc cctgtccacg gactcggtgg agcgcctgcc tgtctacaac
1201 aagtcagcct ggcgccacta ccagaccatc caggaggccg gcgactggtg tgtgcccagc
1261 acggagccca agaacacgtc cacgtaccgc gtcagctaag ggccgccgtg catctgcacc
1321 tgagaggacg gactgccgcc tctgggcccc cccaccgtgg tgcccctcac catcctcctg
1381 ggggagcagg gggtgggttc tccctgatga ccaggttctg tctctatgga agtcactgcg
1441 gtgataggtc tgtgatggtc cctaagtgcc agtccatctc tgtggagacc cctcggtggc
1501 ctccctgtct ctgtgggcga tgcctgaggg ttagggatgt ctccaccctg atggggtgtc
1561 ccagagacat tttcccatgg cagtcctcct ctctgagacc agggctgtca cttttctgcc
1621 aggggtactg ggtccccctc agcaccctcc acagcacagg ccttccaagt ggatgtcccg
1681 ttgccttatt cccccagccc acaaaggcac cctggccttg gcctgctgaa gtgttaggaa
1741 gagggtgggt gccctccaga cctggggact gagtggggaa aggagttaca cccgtgagtg
1801 gggaatgagg ctggtcctgc agcctctccc tccgctcagg gcttgaaggt cggtggcgga
1861 gggggtggct ctcacagggc ccaactctaa agtggaagaa ccttgttaga ccgagagctt
1921 gccatccagc caagctgctc gaggccctgc agtggccttg gcaatgtctg tgccacctcc
1981 tgagccctcc cagcatgtcc tcacatgctc atgcccaccc gctcctccac aagcctagtc
2041 catcctgcct gagctccagc ccccagcccc cactgtgccc agacatgtgt gctcagggtg
2101 gctttctccc taggaccttc tgtgtatata gttagtttta taaccctgaa tgcccccacc
2161 cttcccctaa gcacacaggg gttaaagctg tgtgtccctc ccagtggctg tggcagtgac
2221 agtgacaccc acacccacag taaagaggag actgaatgat caaaaaaaaa aaaaaaaaaa
2281 aaaaaaaaaa a
//