LOCUS BC063035 2061 bp mRNA linear HUM 15-JUL-2006
DEFINITION Homo sapiens GRB2-related adaptor protein, mRNA (cDNA clone
MGC:64880 IMAGE:5757176), complete cds.
ACCESSION BC063035
VERSION BC063035.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2061)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 2061)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (02-DEC-2003) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: Life Technologies, Inc.
cDNA Library Preparation: Life Technologies, Inc.
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Genome Sequence Centre,
BC Cancer Agency, Vancouver, BC, Canada
info@bcgsc.bc.ca
Martin Hirst, Thomas Zeng, Ryan Morin, Michelle Moksa, Johnson
Pang, Diana Mah, Jing Wang, Kieth Fichter, Eric Chuah, Allen
Delaney, Rob Kirkpatrick, Agnes Baross, Sarah Barber, Mabel
Brown-John, Steve S. Chand, William Chow, Ryan Babakaiff, Dave
Wong, Corey Matsuo, Jaclyn Beland, Susan Gibson, Luis delRio, Ruth
Featherstone, Malachi Griffith, Obi Griffith, Ran Guin, Nancy Liao,
Kim MacDonald, Mike R. Mayo, Josh Moran, Diana Palmquist, JR
Santos, Duane Smailus, Jeff Stott, Miranda Tsai, George Yang,
Jacquie Schein, Asim Siddiqui,Steven Jones, Rob Holt, Marco Marra.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 119 Row: i Column: 16
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 50659102.
FEATURES Location/Qualifiers
source 1..2061
/db_xref="H-InvDB:HIT000260742"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:64880 IMAGE:5757176"
/tissue_type="Blood, adult leukocytes"
/clone_lib="NIH_MGC_118"
/lab_host="DH10B"
/note="Vector: pCMV-SPORT6"
gene 1..2061
/gene="GRAP"
/gene_synonym="MGC64880"
/db_xref="GeneID:10750"
/db_xref="HGNC:HGNC:4562"
/db_xref="MIM:604330"
CDS 70..723
/gene="GRAP"
/gene_synonym="MGC64880"
/codon_start=1
/product="GRB2-related adaptor protein"
/protein_id="AAH63035.1"
/db_xref="GeneID:10750"
/db_xref="HGNC:HGNC:4562"
/db_xref="MIM:604330"
/translation="MESVALYSFQATESDELAFNKGDTLKILNMEDDQNWYKAELRGV
EGFIPKNYIRVKPHPWYSGRISRQLAEEILMKRNHLGAFLIRESESSPGEFSVSVNYG
DQVQHFKVLREASGKYFLWEEKFNSLNELVDFYRTTTIAKKRQIFLRDEEPLLKSPGA
CFAQAQFDFSAQDPSQLSFRRGDIIEVLERPDPHWWRGRSCGRVGFFPRSYVQPVHL"
BASE COUNT 469 a 595 c 630 g 367 t
ORIGIN
1 cgactgcaga ggaagcagga agctgtcggc ccagctctga gcccagctgc tggagccccg
61 agcagcggca tggagtccgt ggccctgtac agctttcagg ctacagagag cgacgagctg
121 gccttcaaca agggagacac actcaagatc ctgaacatgg aggatgacca gaactggtac
181 aaggccgagc tccggggtgt cgagggattt attcccaaga actacatccg cgtcaagccc
241 catccgtggt actcgggcag gatttcccgg cagctggccg aagagattct gatgaagcgg
301 aaccatctgg gagccttcct gatccgggag agtgagagct ccccagggga gttctctgtg
361 tctgtgaact atggagacca ggtgcagcac ttcaaggtgc tgcgtgaggc ctcggggaag
421 tacttcctgt gggaggagaa gttcaactcc ctcaacgagc tggtcgactt ctaccgcacc
481 accaccatcg ccaagaagcg gcagatcttc ctgcgcgacg aggagccctt gctcaagtca
541 cctggggcct gctttgccca ggcccagttt gacttctcag cccaggaccc ctcgcagctc
601 agcttccgcc gtggcgacat cattgaggtc ctggagcgcc cagaccccca ctggtggcgg
661 ggccggtcct gcgggcgcgt tggcttcttc ccacggagtt acgtgcagcc cgtgcacctg
721 tgagcagccc ggcggccgat ctggccaacg ggccttttta caggaactga ggtccagaga
781 ggacatggac acccccagct ctgtcagagt cacacggggc tcagtggacg gccttggact
841 gaacgtgggc tcctaactgc ctccggccgc tctgcacaaa ctgggatggc ccaggtcccc
901 cagcaagggt acccagcaag ggtagctccg gggcttcctg gctggttgct ttccattggc
961 tgccagctgt gtgacaccac agggcggagt ctgtggaggc cccgcccacc tcctcttgtc
1021 aatggcccca tctgccagga aggttgagga ctcccaggtt tcacccactg gaggctcaac
1081 ctaaggaacc ctggccatgg tgggtgggtt caccttgggc tgaccaccca ctgggcctgc
1141 ctgcccctcc ctcaaaaggc ccctggagtt gttcaggctg ttgggaggtg gctcagcctc
1201 gaaggacaga ctgcacacct gttgacctaa actcactgga cagactccag gtggacacca
1261 actcctcaac agccccatga cttcagctcc acctggggca cccagcaccc caccacaggg
1321 ctagaggccc taagaccctg aaaaaggggc gggcagtagg gaaggtggag gcacactcct
1381 cttctgttgg acaggtgagc tctgagggca agccctgggc ccagccacgc agagaccctg
1441 gctgccctgg ctcttgtttg tttaggccca ggttctgccc agaccggggc ccagcagagc
1501 tctcaggagg ctgagaaacc tccagggcag acagaaagtt ggggccagag ctaggtttta
1561 gagatcctac aggatcccac atgggacggc tacctaggtg gggcacggcc cctacccgag
1621 aacaggaggg tcctccagag aatgtgccat gatcagggat atgagcaggg cctgcccaga
1681 gcctgggcac ctttgggaga gggaaaagtg aggtgggact tggaaaggct ttgaacgcca
1741 ggctgatggt tgagggatgg tgggcaggga gggagggcga agaggctccc tctggcattt
1801 gggagctggt gggtggaggg aggagccctt ggaggtggct gcagtccctt aatggggggc
1861 cttgctctgg gcatgtgggg ctcttcctcc atccagagct ggcccaagac ccttagaacc
1921 ctgagtgctg gcaaatctca ctgctcctga cccagcctgt gtattcaaaa cgaaaacagt
1981 aaaaccaaca tcaggatgtc cgtcaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
2041 aaaaaaaaaa aaaaaaaaaa a
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