LOCUS BC062688 1217 bp mRNA linear HUM 03-JAN-2005
DEFINITION Homo sapiens protein kinase, cGMP-dependent, type I, mRNA (cDNA
clone IMAGE:30350012), complete cds.
ACCESSION BC062688
VERSION BC062688.1
KEYWORDS .
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1217)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 1217)
AUTHORS Director MGC Project.
TITLE Direct Submission
JOURNAL Submitted (25-NOV-2003) National Institutes of Health, Mammalian
Gene Collection (MGC), Cancer Genomics Office, National Cancer
Institute, 31 Center Drive, Room 11A03, Bethesda, MD 20892-2590,
USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: Dr. Michael Brownstein and Dr. Miklos Palkovits
cDNA Library Preparation: CLONTECH Laboratories, Inc.
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Genome Sequence Centre,
BC Cancer Agency, Vancouver, BC, Canada
info@bcgsc.bc.ca
Steve Jones, Sarah Barber, Mabel Brown-John, Yaron Butterfield,
Andy Chan, Steve S. Chand, William Chow, Alison Cloutier, Ruth
Featherstone, Malachi Griffith, Obi Griffith, Ran Guin, Nancy Liao,
Kim MacDonald, Amara Masson, Mike R. Mayo, Josh Moran, Ryan Morin,
Teika Olson, Diana Palmquist, Anca Petrescu, Anna Liisa Prahbu,
Parvaneh Saeedi, JR Santos, Angelique Schnerch, Ursula Skalska,
Duane Smailus, Jeff Stott, Miranda Tsai, George Yang, Jacquie
Schein, Asim Siddiqui, Rob Holt, Marco Marra.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 51 Row: b Column: 15
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 10835241
This clone has the following problem: The cds is short compared to
the longest cds in the locus.
FEATURES Location/Qualifiers
source 1..1217
/db_xref="H-InvDB:HIT000260579"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="IMAGE:30350012"
/tissue_type="Glandular pool- thyroid, parathyroid,
adrenal cortex, pineal gland, submandibular gland."
/clone_lib="NIH_MGC_184"
/lab_host="DH10B"
/note="Vector: pDNR-LIB"
gene 1..1217
/gene="PRKG1"
/gene_synonym="CGKI"
/gene_synonym="cGKI-alpha"
/gene_synonym="cGKI-BETA"
/gene_synonym="PGK"
/gene_synonym="PRKG1B"
/gene_synonym="PRKGR1B"
/db_xref="GeneID:5592"
/db_xref="MIM:176894"
CDS 28..966
/gene="PRKG1"
/gene_synonym="CGKI"
/gene_synonym="cGKI-alpha"
/gene_synonym="cGKI-BETA"
/gene_synonym="PGK"
/gene_synonym="PRKG1B"
/gene_synonym="PRKGR1B"
/codon_start=1
/product="PRKG1 protein"
/protein_id="AAH62688.1"
/db_xref="GeneID:5592"
/db_xref="MIM:176894"
/translation="MGTLRDLQYALQEKIEELRQRDALIDELELELDQKDELIQKLQN
ELDKYRSVIRPATQQAQKQSASTLQGEPRTKRQAISAEPTAFDIQDLSHVTLPFYPKS
PQSKDLIKEAILDNDFMKNLELSQIQEIVDCMYPVEYGKDSCIIKEGDVGSLVYVMED
GKVEVTKEGVKLCTMGPGKVFGELAILYNCTRTATVKTLVNVKLWAIDRQCFQTIMMR
TGLIKHTEYMEFLKSVPTFQSLPEEILSKLADVLEETHYENGEYIIRQGARGDTFFII
SKGTVNVTREDSPSEDPVFLRTLGKGDWFGEKALQG"
BASE COUNT 367 a 249 c 310 g 291 t
ORIGIN
1 ggggggcccc cggcagcccg gaggagcatg ggcaccttgc gggatttaca gtacgcgctc
61 caggagaaga tcgaggagct gaggcagcgg gatgctctca tcgacgagct ggagctggag
121 ttggatcaga aggacgaact gatccagaag ctgcagaacg agctggacaa gtaccgctcg
181 gtgatccgac cagccaccca gcaggcgcag aagcagagcg cgagcacctt gcagggcgag
241 ccgcgcacca agcggcaggc gatctccgcc gagcccaccg ccttcgacat ccaggatctc
301 agccatgtga ccctgccctt ctaccccaag agcccacagt ccaaggatct tataaaggaa
361 gctatccttg acaatgactt tatgaagaac ttggagctgt cgcagatcca ggagattgtg
421 gattgtatgt acccggtgga gtatggcaag gacagttgca tcatcaaaga aggagacgtg
481 gggtcactgg tgtatgtcat ggaagatggt aaggttgaag ttacaaaaga aggtgtgaag
541 ttgtgtacca tgggtccagg aaaagtgttt ggggaattgg ctattcttta caactgtacc
601 cggacagcga ccgtcaagac tcttgtaaat gtaaaactct gggccattga tcgacaatgt
661 tttcaaacaa taatgatgag gacaggactc atcaagcata ccgagtatat ggaattttta
721 aaaagcgttc caacattcca gagccttcct gaagagatcc tcagcaagct tgctgatgtc
781 cttgaagaga cccactatga aaatggagaa tatattatca ggcaaggtgc aagaggggac
841 accttcttta tcatcagcaa aggaacggta aatgtcactc gtgaagactc accgagtgaa
901 gacccagtct ttcttagaac tttaggaaaa ggagactggt ttggagagaa agccttgcag
961 gggtaagtag atcatgtgtt atacaggttt ttgtttgagt gctacataaa tttctgtctg
1021 aaattttgag ctcctagcac aataggcttt ttatttagtg cttatctcaa tatcaaatat
1081 ggacccttga tgataaatac atttttattt attttcagct ggacttggaa tctgtttgaa
1141 tacgttgtgg aataaattcc ttaatttcta ataaatcagt gttatatcaa aaaaaaaaaa
1201 aaaaaaaaaa aaaaaaa
//