LOCUS BC062554 3823 bp mRNA linear SYN 02-SEP-2016
DEFINITION Synthetic construct Homo sapiens EH-domain containing 2, mRNA (cDNA
clone IMAGE:6455420), **** WARNING: chimeric clone ****.
ACCESSION BC062554
VERSION BC062554.1
KEYWORDS HTC.
SOURCE synthetic construct
ORGANISM synthetic construct
other sequences; artificial sequences.
REFERENCE 1 (bases 1 to 3823)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 3823)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (24-NOV-2003) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC
cDNA Library Preparation: Rubin Laboratory
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: National Institutes of Health Intramural
Sequencing Center (NISC),
Gaithersburg, Maryland;
Web site: http://www.nisc.nih.gov/
Contact: nisc_mgc@nhgri.nih.gov
Akhter,N., Ayele,K., Beckstrom-Sternberg,S.M., Benjamin,B.,
Blakesley,R.W., Bouffard,G.G., Breen,K., Brinkley,C., Brooks,S.,
Dietrich,N.L., Granite,S., Guan,X., Gupta,J., Haghighi,P.,
Hansen,N., Ho,S.-L., Karlins,E., Kwong,P., Laric,P., Legaspi,R.,
Maduro,Q.L., Masiello,C., Maskeri,B., Mastrian,S.D.,McCloskey,J.C.,
McDowell,J., Pearson,R., Stantripop,S., Thomas,P.J., Touchman,J.W.,
Tsurgeon,C., Vogt,J.L., Walker,M.A., Wetherby,K.D., Wiggins,L.,
Young,A., Zhang,L.-H. and Green,E.D.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 50 Row: n Column: 16
This clone was selected for full length sequencing because it
passed the following selection criteria: GenomeScan gene prediction
This clone has the following problem: chimeric.
FEATURES Location/Qualifiers
source 1..3823
/db_xref="H-InvDB:HIT000260466"
/organism="synthetic construct"
/mol_type="mRNA"
/isolation_source="Homo sapiens; Lung, epidermoid
carcinoma"
/db_xref="taxon:32630"
/clone="IMAGE:6455420"
/clone_lib="NIH_MGC_101"
/lab_host="DH10B"
/note="Vector: pOTB7"
BASE COUNT 769 a 1239 c 1068 g 747 t
ORIGIN
1 caaattcccg gccagcggag cggcgagggt ggggactcac gggaagttaa acagcctcgt
61 cggcgtcctc gaggctccaa aaccaggctc taggcgggga cgactgcagc cgttatggag
121 gccaccgcgg ctacggccgc ggctgaggcc tccccaggtg gagcggtggc ctggagggga
181 atcttgatcc tgggccagcc acctgtcaag aggaggcgga gcgtcatgcc tctggaagac
241 tggatgaata ttctccagga gcctgacggg acgtgcgccg ccttgttggt gttgatcagt
301 ccaccaagga gaagcagctg gtcgcagtgg ccgtacctgt aatcccagcg ctttgggagg
361 ccgcggcggg aggatcgctt gagcccggga gttcgagacc agcctgggca acatagtgag
421 aaacccgtcc ctcgtgccga attcggcacg aggctgaagc ggggcggggc acggggccag
481 cagcccgagg ccatccgcac ggtgacctcg gccctcaagg agctgtaccg cacgaagctg
541 ctgccgctgg aggagcacta ccgctttggg gccttccact cgccggccct ggaggacgca
601 gacttcgacg gcaagcccat ggtgctggtg gccggccagt acagcacggg caagaccagc
661 ttcatccagt acctgctgga gcaggaggtg cccggctccc gcgtggggcc tgagcccacc
721 accgactgct ttgtggccgt catgcacggg gacactgagg gcaccgtgcc cggcaacgcc
781 ctcgtcgtgg acccggacaa gcccttccgc aaactcaacc ctttcggaaa caccttcctc
841 aacaggttca tgtgtgccca gctccctaat caggtcctgg agagcatcag catcatcgac
901 accccgggta tcctgtcggg tgccaagcag agagtgagcc gcggctacga cttcccggcc
961 gtgctgcgct ggttcgcgga gcgcgtggac ctcatcatcc tgctctttga tgcgcacaag
1021 ctggagatct cggacgagtt ctcggaggcc atcggcgcgt tgcggggcca tgaggacaag
1081 atccgcgtgg tgctcaacaa ggccgacatg gtggagacgc agcagctgat gcgcgtctac
1141 ggcgcgctca tgtgggcgct gggcaaggtg gtgggcacgc ccgaggtgct gcgcgtctac
1201 atcggctcct tctggtccca gcccctcctc gtgcccgaca accggcgcct cttcgagctg
1261 gaggagcagg acctcttccg cgacatccag ggcctgcccc ggcacgcagc cttgcgcaag
1321 ctcaacgacc tggtgaagag ggcccggctg gtgcgagttc acgcttacat catcagctac
1381 ctgaagaagg agatgccctc tgtgtttggg aaggagaaca agaagaagca gctgatcctc
1441 aaactgcccg tcatctttgc gaagattcag ctggaacatc acatctcccc tggggacttt
1501 cctgattgcc agaaaatgca ggagctgctg atggcgcacg acttcaccaa gtttcactcg
1561 ctgaagccga agctgctgga ggcactggac gagatgctga cgcacgacat cgccaagctc
1621 atgcccctgc tgcggcagga ggagctggag agcaccgagg tgggcgtgca ggggggcgct
1681 tttgagggca cccacatggg cccgtttgtg gagcggggac ctgacgaggc catggaggac
1741 ggcgaggagg gctcggacga cgaggccgag tgggtggtga ccaaggacaa gtccaaatac
1801 gacgagatct tctacaacct ggcgcctgcc gacggcaagc tgagcggctc caaggccaag
1861 acctggatgg tggggaccaa gctccccaac tcagtgctgg ggcgcatctg gaagctcagc
1921 gatgtggacc gcgacggcat gctggatgat gaagagttcg cgctggccag ccacctcatc
1981 gaggccaagc tggaaggcca cgggctgccc gccaacctgc cccgtcgcct ggtgccaccc
2041 tccaagcgac gccacaaggg ctccgccgag tgagccgggc ccccctccca tggccctgct
2101 gtggctcccc agctccagtc ggctgcacgc acacccctgc tccggctcac acacgccctg
2161 cctgccctcc ctgcccagct gtaaggaccg ggggtctccc tcctcactac cgccagacac
2221 cccggtggaa gcatttagag gggaccacgg gagggacaag gcttctctgt ccgcccttca
2281 cacctccagc ctcacgttca cttaggcaca tcacacacac actggcacac gcaggcatcc
2341 atccatccgt cattcattca aatatttatt gagcacctac tatgtgccca gccctgttct
2401 aggcactggg cattaccata gagaacaaaa tagacaaata catctgccct catggaaggt
2461 gacgttccca ggagagggca cctacacagt cacgcaaaca cacactaatt cctggcaggg
2521 cccccagccc ctcccctggc tgagcagccc tgtggctgaa atgactagca gataaacaga
2581 cccccttctg ctccgcttcc tcctgcccag ccaggcaaca ccctcaaccg gctccatcac
2641 atcctcaggt ctcgggacca tggggggctc agaggggaga cacacctact gcttcctcag
2701 atgggcccct ccgcagcccc ttcccttgct cggggaaagc ccccaattct gcccacaccc
2761 atttatttcc ttccttcctt ccttcttttc tttccttcct tccttctttt ttgtttttgc
2821 ccccaattct gcccataccc atttctttct ttccttcctt ccttcttttt tgtttttgcc
2881 cccagttctg tccacacccc ttccctttcc tgtcctgtcc tttctttctt tcttttttga
2941 tagaatcttg ctctgtcgcc caggctggag tgcagtggtg agatctcagc tcactgcaac
3001 ctccacctcc tgggttgaag tgattctcgt gcctcagcct cctgagtagc tgggactgca
3061 ggcacgcgcc accacgccca gctaattttt gtatttgagt agagacgggg tttcaccatg
3121 ttggccaggc tggtctcgaa ctccgcatct caggtgatct gctcgcctcg gcctcccaaa
3181 gtgatgggat tacaggcatg agccaccgtg cccggcttca cacccatttc tttaaaaagg
3241 atcccgtagc aggcagaaaa gccccttcca tcctgctcct ctgatactgt gcccccttgg
3301 agatatttcc gtcctccacc cacgtgtctg tggctggaac tgcccagcct gctcctggcc
3361 ccctggaagc ctccccacag ctggtaatct ggacttaagg attgctgggc caccgcctct
3421 ctgcctacca ccattccata tttaagtgga gcccctacgt agaaaggccc cggggcttta
3481 ttttagtctc cttttcaggg atgtcgtggg cgggggaggg ggttcttggt gctacagccc
3541 tctccccacc cctaaaggga cgccgacgct gtttgctgcc ttcaccacat attagtgctt
3601 gaccctggca ggggacccca tggaaaagat ggggaagagc aaaatacatg gagacgacgc
3661 accctccagg atgctcgctg ggattcccac gcccaccact gtcccccacc ccatggctgg
3721 gaggggcctc tgaacggaac agtgtcccca cagagcgaat aaagccaagg cttcttccca
3781 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaa
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