LOCUS BC062339 2992 bp mRNA linear HUM 21-JUL-2006
DEFINITION Homo sapiens alkB, alkylation repair homolog 5 (E. coli), mRNA
(cDNA clone MGC:71143 IMAGE:6339184), complete cds.
ACCESSION BC062339
VERSION BC062339.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2992)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 2992)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (17-NOV-2003) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC
cDNA Library Preparation: Rubin Laboratory
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: National Institutes of Health Intramural
Sequencing Center (NISC),
Gaithersburg, Maryland;
Web site: http://www.nisc.nih.gov/
Contact: nisc_mgc@nhgri.nih.gov
Akhter,N., Ayele,K., Beckstrom-Sternberg,S.M., Benjamin,B.,
Blakesley,R.W., Bouffard,G.G., Breen,K., Brinkley,C., Brooks,S.,
Dietrich,N.L., Granite,S., Guan,X., Gupta,J., Haghighi,P.,
Hansen,N., Ho,S.-L., Karlins,E., Kwong,P., Laric,P., Legaspi,R.,
Maduro,Q.L., Masiello,C., Maskeri,B., Mastrian,S.D.,McCloskey,J.C.,
McDowell,J., Pearson,R., Stantripop,S., Thomas,P.J., Touchman,J.W.,
Tsurgeon,C., Vogt,J.L., Walker,M.A., Wetherby,K.D., Wiggins,L.,
Young,A., Zhang,L.-H. and Green,E.D.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 50 Row: m Column: 12
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 40353209.
FEATURES Location/Qualifiers
source 1..2992
/db_xref="H-InvDB:HIT000260339"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:71143 IMAGE:6339184"
/tissue_type="Brain, neuroblastoma"
/clone_lib="NIH_MGC_47"
/lab_host="DH10B-R"
/note="Vector: pOTB7"
gene 1..2992
/gene="ALKBH5"
/gene_synonym="FLJ20308"
/gene_synonym="OFOXD"
/db_xref="GeneID:54890"
/db_xref="HGNC:HGNC:25996"
CDS 270..1646
/gene="ALKBH5"
/gene_synonym="FLJ20308"
/gene_synonym="OFOXD"
/codon_start=1
/product="alkB, alkylation repair homolog 5 (E. coli)"
/protein_id="AAH62339.1"
/db_xref="GeneID:54890"
/db_xref="HGNC:HGNC:25996"
/translation="MAAASGYTDLREKLKSMTSRDNYKAGSREAAAAAAAAVAAAAAA
AAAAEPYPVSGAKRKYQEDSDPERSDYEEQQLQKEEEARKVKSGIRQMRLFSQDECAK
IEARIDEVVSRAEKGLYNEHTVDRAPLRNKYFFGEGYTYGAQLQKRGPGQERLYPPGD
VDEIPEWVHQLVIQKLVEHRVIPEGFVNSAVINDYQPGGCIVSHVDPIHIFERPIVSV
SFFSDSALCFGCKFQFKPIRVSEPVLSLPVRRGSVTVLSGYAADEITHCIRPQDIKER
RAVIILRKTRLDAPRLETKSLSSSVLPPSYASDRLSGNNRDPALKPKRSHRKADPDAA
HRPRILEMDKEENRRSVLLPTHRRRGSFSSENYWRKSYESSEDCSEAAGSPGNSGSSL
RSCPSFCFEGFVFVHWGVFVFCFLFFLILYIFPWFCCLLGLKNRIGQDLGSHILGIPP
GWKGCWHQ"
BASE COUNT 614 a 850 c 826 g 702 t
ORIGIN
1 gaggcgctgc tggcgcccct gccccgcggg acgtggagaa ggtggaggag gaagaagccc
61 cgttgtcgcc accgttgcat gacccgccgc tcctgaggcc ctaccccacg cccggaccct
121 cgacgccccc cgccgggtcc cccactcacg catgggggtt cggcgctaag gacccccctc
181 cctccggggg ccccggggcg cgtcccctta gagccatgcc cggctgcccc gcccgccccg
241 gaggacccta gagcagcgtc gtgggggcca tggcggccgc cagcggctac acggacctgc
301 gtgagaagct caagtccatg acgtcccggg acaactataa ggcgggcagc cgggaggccg
361 ccgccgctgc cgcagccgcc gtagccgccg cagccgcagc cgccgctgcc gccgaacctt
421 accctgtgtc cggggccaag cgcaagtatc aggaggactc ggaccccgag cgcagcgact
481 atgaggagca gcagctgcag aaggaggagg aggcgcgcaa ggtgaagagc ggcatccgcc
541 agatgcgcct cttcagccag gacgagtgcg ccaagatcga ggcccgcatt gacgaggtgg
601 tgtcccgcgc tgagaagggc ctgtacaacg agcacacggt ggaccgggcc ccactgcgca
661 acaagtactt cttcggcgaa ggctacactt acggcgccca gctgcagaag cgcgggcccg
721 gccaggagcg cctctacccg ccgggcgacg tggacgagat ccccgagtgg gtgcaccagc
781 tggtgatcca aaagctggtg gagcaccgcg tcatccccga gggcttcgtc aacagcgccg
841 tcatcaacga ctaccagccc ggcggctgca tcgtgtctca cgtggacccc atccacatct
901 tcgagcgccc catcgtgtcc gtgtccttct ttagcgactc tgcgctgtgc ttcggctgca
961 agttccagtt caagcctatt cgggtgtcgg aaccagtgct ttccctgccg gtgcgcaggg
1021 gaagcgtgac tgtgctcagt ggatatgctg ctgatgaaat cactcactgc atacggcctc
1081 aggacatcaa ggagcgccga gcagtcatca tcctcaggaa gacaagatta gatgcacccc
1141 ggttggaaac aaagtccctg agcagctccg tgttaccacc cagctatgct tcagatcgcc
1201 tgtcaggaaa caacagggac cctgctctga aacccaagcg gtcccaccgc aaggcagacc
1261 ctgatgctgc ccacaggcca cggattctgg agatggacaa ggaagagaac cggcgctcgg
1321 tgctgctgcc cacacaccgg cggaggggta gcttcagctc tgagaactac tggcgcaagt
1381 catacgagtc ctcagaggac tgctctgagg cagcaggcag ccctgggaac tctggctcat
1441 ccttacgtag ttgcccctcc ttttgttttg agggttttgt ttttgttcat tggggggttt
1501 ttgttttttg ttttttgttt tttttgattc tatatatttt tccttggttt tgttgcctgt
1561 tagggctgaa gaatagaatt ggccaggacc taggttctca tattcttggt attcctcctg
1621 gatggaaagg ctgttggcat caatagggga cagaggctga tgctggagtg gccagtagag
1681 gtggtggagc agagcagcca tcttttaagt ggggctgtat caggctgggt ttatttaaaa
1741 gcaacaaaat gttttggtta agaaaattat tttgctttca gtgtaaatct tcgcagtgtt
1801 ctaaacaaag ttcagtcttc tgctcgcccc tttccctcac tgatgtctgc acttggttga
1861 ggtctcctgg agcctcacag gctctgctgt tctccacttc tcacctgcca tccacgccct
1921 gcaagctcat gcaaacaccc tttcttcctc ctgcggcaga gttgttcagg ttgcctgggc
1981 aggggcttaa acagtgccag cccctgccat cccaaagcta ttgttaagcc ccccaggcgt
2041 cctccaccca cgcccactag cctgccatgt ccacagttcc ttgggctgct gaggggctag
2101 tgcagtggtc ctgacctctc ttatcaggag cacacttctt tgctggttgc tccttttgag
2161 catatgcgtg tgattatttg gaacagttag acttgccacg ttgggtcagt tttagaaatt
2221 gtttctagct agagggactg gtgtccttcc aagtctagca tttggggtat ggaaaattgt
2281 tgtggtgtgt ggtagggttt ttgttttctt ttttgagttt ttttttcccc ctttagtctc
2341 ctggcttttt cctttccctt cccttctcca ctggccagct tgggcctcat cctcatgtca
2401 tccttctagg aaggcgcctg ccccatcttg tctgccggca gcatgcatcc aaggccagag
2461 ctcaggcctg cagactgggc tggtgcctcc tccgcttcag ggtatgggag ttggtgaagg
2521 ggctttcaaa aaataataag gaaaaaaagg taaagtcttt ggtagcttct atccactcag
2581 atcctggaag gcagcaaggt tttgtggatc tagattcatt aggaatgtct tcttgtcagc
2641 caggccagga cccgggcttg ccaagagcag aggccctccc agcaaccagg ataccaccac
2701 tttgggggct ttgtgtacag aggtccgggt ctgagacctc ataggctgca gaaatctggg
2761 gcagccacca tcaagaagcc cctctcaggg gccagaactc ctttgccagc gtggatttct
2821 caagtcggga ctgcataatt aaagcagttg cagttttatt ttttttacag cttttttccc
2881 aaaaatgatt tgtagttgtg tgtgcagcac ttcgccctga tatgtgtgct ctacaataaa
2941 aaccaaatct aatatatttt gaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aa
//