LOCUS BC061696 1850 bp mRNA linear HUM 15-JUL-2006
DEFINITION Homo sapiens ATG4 autophagy related 4 homolog A (S. cerevisiae),
mRNA (cDNA clone MGC:62085 IMAGE:4594225), complete cds.
ACCESSION BC061696
VERSION BC061696.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1850)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 1850)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (05-NOV-2003) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: CLONTECH
cDNA Library Preparation: CLONTECH Laboratories, Inc.
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: National Institutes of Health Intramural
Sequencing Center (NISC),
Gaithersburg, Maryland;
Web site: http://www.nisc.nih.gov/
Contact: nisc_mgc@nhgri.nih.gov
Akhter,N., Ayele,K., Beckstrom-Sternberg,S.M., Benjamin,B.,
Blakesley,R.W., Bouffard,G.G., Breen,K., Brinkley,C., Brooks,S.,
Dietrich,N.L., Granite,S., Guan,X., Gupta,J., Haghighi,P.,
Hansen,N., Ho,S.-L., Karlins,E., Kwong,P., Laric,P., Legaspi,R.,
Maduro,Q.L., Masiello,C., Maskeri,B., Mastrian,S.D.,McCloskey,J.C.,
McDowell,J., Pearson,R., Stantripop,S., Thomas,P.J., Touchman,J.W.,
Tsurgeon,C., Vogt,J.L., Walker,M.A., Wetherby,K.D., Wiggins,L.,
Young,A., Zhang,L.-H. and Green,E.D.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 48 Row: d Column: 20
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 30795249.
FEATURES Location/Qualifiers
source 1..1850
/db_xref="H-InvDB:HIT000260228"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:62085 IMAGE:4594225"
/tissue_type="Kidney"
/clone_lib="NIH_MGC_75"
/lab_host="DH10B"
/note="Vector: pDNR-LIB"
gene 1..1850
/gene="ATG4A"
/db_xref="GeneID:115201"
/db_xref="HGNC:HGNC:16489"
CDS 163..1359
/gene="ATG4A"
/codon_start=1
/product="ATG4 autophagy related 4 homolog A (S.
cerevisiae)"
/protein_id="AAH61696.1"
/db_xref="GeneID:115201"
/db_xref="HGNC:HGNC:16489"
/translation="MESVLSKYEDQITIFTDYLEEYPDTDELVWILGKQHLLKTEKSK
LLSDISARLWFTYRRKFSPIGGTGPSSDAGWGCMLRCGQMMLAQALICRHLGRDWSWE
KQKEQPKEYQRILQCFLDRKDCCYSIHQMAQMGVGEGKSIGEWFGPNTVAQVLKKLAL
FDEWNSLAVYVSMDNTVVIEDIKKMCRVLPLSADTAGDRPPDSLTASNQSKGTSAYCS
AWKPLLLIVPLRLGINQINPVYVDAFKECFKMPQSLGALGGKPNNAYYFIGFLGDELI
FLDPHTTQTFVDTEENGTVNDQTFHCLQSPQRMNILNLDPSVALGFFCKEEKDFDNWC
SLVQKEILKENLRMFELVQKHPSHWPPFVPPAKPEVTTTGAEFIDSTEQLEEFDLEED
FEILSV"
BASE COUNT 576 a 388 c 418 g 468 t
ORIGIN
1 gggggagttg cagtttgaga gcagttccgg gcagggaggc gcctttgctg ccctcacaga
61 cttggcccct agcagtgcag aactacaagt cccagggatc ctagcgaccg tccgtccgta
121 gtcaagttgc cggtggaatt ggcccaggat gacagctgga gaatggagtc agttttatcc
181 aagtatgaag atcagattac tattttcact gactacctag aagaatatcc agatacagat
241 gagctggtat ggatcttagg gaagcagcat ctccttaaaa cagaaaaatc taagctgttg
301 tctgatataa gtgctcgtct atggtttaca tacagaagga aattttcacc aattggtgga
361 acgggccctt catcagatgc tggttgggga tgtatgctac gctgtggaca gatgatgctg
421 gctcaagccc ttatctgtag acacttggga agggactgga gctgggagaa acaaaaagaa
481 caacccaaag aataccaacg catcctacag tgcttcttag atagaaaaga ttgttgctac
541 tctatccatc aaatggcaca aatgggtgta ggagaaggga aatcaattgg agaatggttt
601 ggaccaaata cagttgcaca ggtgttaaaa aaacttgctt tatttgacga atggaattcc
661 ttggctgttt atgtttcaat ggataacaca gtggtcattg aagatatcaa aaaaatgtgc
721 cgtgtccttc ccttgagtgc tgacacagct ggtgacaggc ctcccgattc tttaactgct
781 tcaaaccaga gtaagggcac ctctgcctac tgctcagcct ggaaacccct gctgctcatt
841 gtgccccttc gcctgggcat aaaccaaatc aatcctgtct atgttgatgc attcaaagag
901 tgttttaaga tgccacagtc tttaggggca ttaggaggaa aaccaaataa cgcgtattat
961 ttcataggat tcttaggtga cgagctcatc ttcttggacc ctcatacaac ccagaccttt
1021 gttgacactg aagagaatgg aacggttaat gaccagactt tccattgcct gcagtcccca
1081 cagcgaatga acatcctaaa cctggatcct tcagttgcat tgggattttt ctgcaaagaa
1141 gaaaaagact ttgataactg gtgtagcctt gttcagaagg aaattctaaa ggagaattta
1201 aggatgtttg aattagttca gaaacatcca tcacactggc ctccctttgt acctccagcc
1261 aagccagaag tgacaaccac tggggcagaa ttcattgact ctactgagca actggaggag
1321 tttgatctgg aggaagattt tgagattctg agtgtgtaga atcctgggaa ctcaacttga
1381 aggtctgtct tccatctggc accataaaaa catgaactta ttgcataaaa cttttctagt
1441 cagcaagtgc ctgatatgcc aatagcatac aaactcaata gcaatcatga ctgagccaat
1501 cactgtttct cagaaaaaca aaacaaaaca aaacaaatga cagtaaccct tccccggaaa
1561 gaaatagaac aatcatggag cctaggagca gagagatgag gaggagttca ttgcttccca
1621 gcttgtgtta tatggctaca gcaagtcttc agctgctgca atgaggaaat gggcatctgg
1681 aagacaaaca gcaactctca gcttgcttca agaaccagca gataagagat ggttaagctg
1741 ttcttcaccc tttcagatgt gacctctttt ggactaagca gcaatctgtt ctcttgctca
1801 aataataaag tgactgaatc agaaaaaaaa aaaaaaaaaa aaaaaaaaaa
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