LOCUS BC058924 1053 bp mRNA linear HUM 15-JUL-2006
DEFINITION Homo sapiens ubiquitin-conjugating enzyme E2M (UBC12 homolog,
yeast), mRNA (cDNA clone MGC:65116 IMAGE:6162220), complete cds.
ACCESSION BC058924
VERSION BC058924.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1053)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 1053)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (01-OCT-2003) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC/DCTD/DTP
cDNA Library Preparation: Life Technologies, Inc.
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Genome Sequence Centre,
BC Cancer Agency, Vancouver, BC, Canada
info@bcgsc.bc.ca
Martin Hirst, Thomas Zeng, Ryan Morin, Michelle Moksa, Johnson
Pang, Diana Mah, Jing Wang, Kieth Fichter, Eric Chuah, Allen
Delaney, Rob Kirkpatrick, Agnes Baross, Sarah Barber, Mabel
Brown-John, Steve S. Chand, William Chow, Ryan Babakaiff, Dave
Wong, Corey Matsuo, Jaclyn Beland, Susan Gibson, Luis delRio, Ruth
Featherstone, Malachi Griffith, Obi Griffith, Ran Guin, Nancy Liao,
Kim MacDonald, Mike R. Mayo, Josh Moran, Diana Palmquist, JR
Santos, Duane Smailus, Jeff Stott, Miranda Tsai, George Yang,
Jacquie Schein, Asim Siddiqui,Steven Jones, Rob Holt, Marco Marra.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 119 Row: m Column: 23
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 37577133.
FEATURES Location/Qualifiers
source 1..1053
/db_xref="H-InvDB:HIT000259934"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:65116 IMAGE:6162220"
/tissue_type="Skin, melanotic melanoma."
/clone_lib="NIH_MGC_72"
/lab_host="DH10B"
/note="Vector: pCMV-SPORT6"
gene 1..1053
/gene="UBE2M"
/gene_synonym="hUbc12"
/gene_synonym="UBC-RS2"
/gene_synonym="UBC12"
/db_xref="GeneID:9040"
/db_xref="HGNC:HGNC:12491"
/db_xref="MIM:603173"
CDS 110..661
/gene="UBE2M"
/gene_synonym="hUbc12"
/gene_synonym="UBC-RS2"
/gene_synonym="UBC12"
/codon_start=1
/product="ubiquitin-conjugating enzyme E2M (UBC12 homolog,
yeast)"
/protein_id="AAH58924.1"
/db_xref="GeneID:9040"
/db_xref="HGNC:HGNC:12491"
/db_xref="MIM:603173"
/translation="MIKLFSLKQQKKEEESAGGTKGSSKKASAAQLRIQKDINELNLP
KTCDISFSDPDDLLNFKLVICPDEGFYKSGKFVFSFKVGQGYPHDPPKVKCETMVYHP
NIDLEGNVCLNILREDWKPVLTINSIIYGLQYLFLEPNPEDPLNKEAAEVLQNNRRLF
EQNVQRSMRGGYIGSTYFERCLK"
BASE COUNT 221 a 304 c 330 g 198 t
ORIGIN
1 ggccgcagct gcgggtccga ggagcggagg cgacgcgggc ggcggcgggg ggccgggtgg
61 ccggggtccc gggccccgcg gcggcggcag cggcggcggc ggcggcagga tgatcaagct
121 gttctcgctg aagcagcaga agaaggagga ggagtcggcg ggcggcacca agggcagcag
181 caagaaggcg tcggcggcgc agctgcggat ccagaaggac ataaacgagc tgaacctgcc
241 caagacgtgt gatatcagct tctcagatcc agacgacctc ctcaacttca agctggtcat
301 ctgtcctgat gagggcttct acaagagtgg gaagtttgtg ttcagtttta aggtgggcca
361 gggttacccg catgatcccc ccaaggtgaa gtgtgagaca atggtctatc accccaacat
421 tgacctcgag ggcaacgtct gcctcaacat cctcagagag gactggaagc cagtccttac
481 gataaactcc ataatttatg gcctgcagta tctcttcttg gagcccaacc ccgaggaccc
541 actgaacaag gaggccgcag aggtcctgca gaacaaccgg cggctgtttg agcagaacgt
601 gcagcgctcc atgcggggtg gctacatcgg ctccacctac tttgagcgct gcctgaaata
661 gggttggcgc atacccaccc ccgccacggc cacaagccct ggcatcccct gcaaatattt
721 attgggggcc atgggtaggg gtttgggggg cggccggtgg gggaatcccc tgccttggcc
781 ttgcctcccc ttcctgccac gtgcccctag ttattttttt tttttaacac catgtgatta
841 aggtcggcgc tgcctccccc gacccactca gcgatgggaa atgaattggc ttgtctagcc
901 cccctgctgg gtgcttgttc agcccccact ctgggctgtg gagtgggtgg gcaacgggcc
961 tgggtagctg ggcccaggca acccacccct ccacctctgg aggtcccacc aggctattaa
1021 aggggaatgt tactgcaaaa aaaaaaaaaa aaa
//