LOCUS BC056898 3122 bp mRNA linear HUM 19-JAN-2018
DEFINITION Homo sapiens plastin 3 (T isoform), mRNA (cDNA clone MGC:65051
IMAGE:6064540), complete cds.
ACCESSION BC056898
VERSION BC056898.2
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 3122)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 3122)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (25-AUG-2003) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
REFERENCE 3 (bases 1 to 3122)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (19-JAN-2018) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK Sequence update by database staff to remove vector contamination
COMMENT On Jan 19, 2018 this sequence version replaced BC056898.1.
Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC/DCTD/DTP
cDNA Library Preparation: Life Technologies, Inc.
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Genome Sequence Centre,
BC Cancer Agency, Vancouver, BC, Canada
info@bcgsc.bc.ca
Martin Hirst, Thomas Zeng, Ryan Morin, Michelle Moksa, Johnson
Pang, Diana Mah, Jing Wang, Kieth Fichter, Eric Chuah, Allen
Delaney, Rob Kirkpatrick, Agnes Baross, Sarah Barber, Mabel
Brown-John, Steve S. Chand, William Chow, Ryan Babakaiff, Dave
Wong, Corey Matsuo, Jaclyn Beland, Susan Gibson, Luis delRio, Ruth
Featherstone, Malachi Griffith, Obi Griffith, Ran Guin, Nancy Liao,
Kim MacDonald, Mike R. Mayo, Josh Moran, Diana Palmquist, JR
Santos, Duane Smailus, Jeff Stott, Miranda Tsai, George Yang,
Jacquie Schein, Asim Siddiqui,Steven Jones, Rob Holt, Marco Marra.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 119 Row: k Column: 9
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 28416938.
FEATURES Location/Qualifiers
source 1..3122
/db_xref="H-InvDB:HIT000259623"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:65051 IMAGE:6064540"
/tissue_type="Skin, melanotic melanoma."
/clone_lib="NIH_MGC_72"
/lab_host="DH10B"
/note="Vector: pCMV-SPORT6"
gene 1..3122
/gene="PLS3"
/gene_synonym="T-PLASTIN"
/db_xref="GeneID:5358"
/db_xref="HGNC:HGNC:9091"
/db_xref="MIM:300131"
CDS 105..1997
/gene="PLS3"
/gene_synonym="T-PLASTIN"
/codon_start=1
/product="plastin 3 (T isoform)"
/protein_id="AAH56898.1"
/db_xref="GeneID:5358"
/db_xref="HGNC:HGNC:9091"
/db_xref="MIM:300131"
/translation="MDEMATTQISKDELDELKEAFAKVDLNSNGFICDYELHELFKEA
NMPLPGYKVREIIQKLMLDGDRNKDGKISFDEFVYIFQEVKSSDIAKTFRKAINRKEG
ICALGGTSELSSEGTQHSYSEEEKYAFVNWINKALENDPDCRHVIPMNPNTDDLFKAV
GDGIVLCKMINLSVPDTIDERAINKKKLTPFIIQENLNLALNSASAIGCHVVNIGAED
LRAGKPHLVLGLLWQIIKIGLFADIELSRNEALAALLRDGETLEELMKLSPEELLLRW
ANFHLENSGWQKINNFSADIKDSKAYFHLLNQIAPKGQKEGEPRIDINMSGFNETDDL
KRAESMLQQADKLGCRQFVTPADVVSGNPKLNLAFVANLFNKYPALTKPENQDIDWTL
LEGETREERTFRNWMNSLGVNPHVNHLYADLQDALVILQLYERIKVPVDWSKVNKPPY
PKLGANMKKLENCNYAVELGKHPAKFSLVGIGGQDLNDGNQTLTLALVWQLMRRYTLN
VLEDLGDGQKANDDIIVNWVNRTLSEAGKSTSIQSFKDKTISSSLAVVDLIDAIQPGC
INYDLVKSGNLTEDDKHNNAKYAVSMARRIGARVYALPEDLVEVKPKMVMTVFACLMG
RGMKRV"
BASE COUNT 972 a 566 c 662 g 922 t
ORIGIN
1 tttagagcca cagctgcaaa gattccgagg tgcagaagtt gtctgagtgc gttggtcggc
61 ggcagtcggg ccagacccag gactctgcga ctttacatct ttaaatggat gagatggcta
121 ccactcagat ttccaaagat gagcttgatg aactcaaaga ggcctttgca aaagttgatc
181 tcaacagcaa cggattcatt tgtgactatg aacttcatga gctcttcaag gaagctaata
241 tgccattacc aggatataaa gtgagagaaa ttattcagaa actcatgctg gatggtgaca
301 ggaataaaga tgggaaaata agttttgacg aatttgttta tatttttcaa gaggtaaaaa
361 gtagtgatat tgccaagacc ttccgcaaag caatcaacag gaaagaaggt atttgtgctc
421 tgggtggaac ttcagagttg tccagcgaag gaacacagca ttcttactca gaggaagaaa
481 aatatgcttt tgttaactgg ataaacaaag ctttggaaaa tgatcctgat tgtagacatg
541 ttataccaat gaaccctaac accgatgacc tgttcaaagc tgttggtgat ggaattgtgc
601 tttgtaaaat gattaacctt tcagttcctg ataccattga tgaaagagca atcaacaaga
661 agaaacttac acccttcatc attcaggaaa acttgaactt ggcactgaac tctgcttctg
721 ccattgggtg tcatgttgtg aacattggtg cagaagattt gagggctggg aaacctcatc
781 tggttttggg actgctttgg cagatcatta agatcggttt gttcgctgac attgaattaa
841 gcaggaatga agccttggct gctttactcc gagatggtga gactttggag gaacttatga
901 aattgtctcc agaagagctt ctgcttagat gggcaaactt tcatttggaa aactcgggct
961 ggcaaaaaat taacaacttt agtgctgaca tcaaggattc caaagcctat ttccatcttc
1021 tcaatcaaat cgcaccaaaa ggacaaaagg aaggtgaacc acggatagat attaacatgt
1081 caggtttcaa tgaaacagat gatttgaaga gagctgagag tatgcttcaa caagcagata
1141 aattaggttg cagacagttt gttacccctg ctgatgttgt cagtggaaac cccaaactca
1201 acttagcttt cgtggctaac ctgtttaata aatacccagc actaactaag ccagagaacc
1261 aggatattga ctggactcta ttagaaggag aaactcgtga agaaagaacc ttccgtaact
1321 ggatgaactc tcttggtgtc aatcctcacg taaaccatct ctatgctgac ctgcaagatg
1381 ccctggtaat cttacagtta tatgaacgaa ttaaagttcc tgttgactgg agtaaggtta
1441 ataaacctcc atacccgaaa ctgggagcca acatgaaaaa gctagaaaac tgcaactatg
1501 ctgttgaatt agggaagcat cctgctaaat tctccctggt tggcattgga gggcaagacc
1561 tgaatgatgg gaaccaaacc ctgactttag ctttagtctg gcagctgatg agaagatata
1621 ccctcaatgt cctggaagat cttggagatg gtcagaaagc caatgacgac atcattgtga
1681 actgggtgaa cagaacgttg agtgaagctg gaaaatcaac ttccattcag agttttaagg
1741 acaagacgat cagctccagt ttggcagttg tggatttaat tgatgccatc cagccaggct
1801 gtataaacta tgaccttgtg aagagtggca atctaacaga agatgacaag cacaataatg
1861 ccaagtatgc agtgtcaatg gctagaagaa tcggagccag agtgtatgct ctccctgaag
1921 accttgtgga agtaaagccc aagatggtca tgactgtgtt tgcatgtttg atgggcaggg
1981 gaatgaagag agtgtaaaat aaccaatctg aataaaacag ccatgctccc aggtgcatga
2041 ttcgcaggtc agctatttcc aggtgaagtg cttatggctt aaggaactct tggccattca
2101 aaggactttt cattttgatt aacaggacta gcttatcatg agagccctca ggggaaaggg
2161 tttaagaaaa acaactcctc tttcccatag tcagagttga atttgtcagg cacgcctgaa
2221 atgtgctcat agccaaaaca ttttactctc tcctcctaga atgctgccct tgacatttcc
2281 cattgctgta tgttatttct tgctctgtta tcttttgccc tcttagaatg tccctctctt
2341 gggacttgct tagatgatgg gatatgaata ttattagaca gtaattttgc tttccatcca
2401 gtatgctagt tcttattcga gaactatggt cagagcgtat ttggatatga gtatcctttg
2461 cttatctttg tagtactgaa aatttgccga agtaactggc tgtgcagaat gtaatagaag
2521 cttttcttat tcttttattc ttaagatcag tatcttttta cagtattctt tctacatgat
2581 ccttttttgt acatttaaga atattttgat tatattaaac aagactgctg attttgctac
2641 tttttttaag gggtcttcaa gtaagtaaaa catacatcgt agctagaaga aaaatgtacc
2701 ttaaatttgc atcttccctc tcatacccaa gctgtaaaca attgaaatat tttgtcttaa
2761 atcacttggt tcaatacatg cttatttgtt ttaaaacctg tatcatcaaa ctctctctct
2821 aaatttaaaa tgctgttgaa tatgatactt ttgaggagag agtgtgctca gaacttagac
2881 gggatttggt aggccaagta tgctaagtgt acaatatatt ttttaatttt acacctgaaa
2941 caaagaaatg tggtcactaa aaataaaagt atatatgtag gaattaatgt actcttgctt
3001 tgtcaagctg tttgctatag tttccaaggt attatgttac tctaactctg aaaagtgatg
3061 taatctggta gcaatgtagt agttcaaata aaggcattta cataataaaa aaaaaaaaaa
3121 aa
//