LOCUS BC056249 1220 bp mRNA linear SYN 02-SEP-2016
DEFINITION Synthetic construct Homo sapiens proteasome (prosome, macropain)
subunit, alpha type, 4, mRNA (cDNA clone MGC:61812 IMAGE:4093514),
complete cds.
ACCESSION BC056249
VERSION BC056249.1
KEYWORDS MGC.
SOURCE synthetic construct
ORGANISM synthetic construct
other sequences; artificial sequences.
REFERENCE 1 (bases 1 to 1220)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 1220)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (11-AUG-2003) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC
cDNA Library Preparation: CLONTECH Laboratories, Inc.
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: National Institutes of Health Intramural
Sequencing Center (NISC),
Gaithersburg, Maryland;
Web site: http://www.nisc.nih.gov/
Contact: nisc_mgc@nhgri.nih.gov
Akhter,N., Ayele,K., Beckstrom-Sternberg,S.M., Benjamin,B.,
Blakesley,R.W., Bouffard,G.G., Breen,K., Brinkley,C., Brooks,S.,
Dietrich,N.L., Granite,S., Guan,X., Gupta,J., Haghighi,P.,
Hansen,N., Ho,S.-L., Karlins,E., Kwong,P., Laric,P., Legaspi,R.,
Maduro,Q.L., Masiello,C., Maskeri,B., Mastrian,S.D.,McCloskey,J.C.,
McDowell,J., Pearson,R., Stantripop,S., Thomas,P.J., Touchman,J.W.,
Tsurgeon,C., Vogt,J.L., Walker,M.A., Wetherby,K.D., Wiggins,L.,
Young,A., Zhang,L.-H. and Green,E.D.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 48 Row: a Column: 22
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 23110940.
FEATURES Location/Qualifiers
source 1..1220
/db_xref="H-InvDB:HIT000054145"
/organism="synthetic construct"
/mol_type="mRNA"
/isolation_source="Homo sapiens; Brain, glioblastoma"
/db_xref="taxon:32630"
/clone="MGC:61812 IMAGE:4093514"
/clone_lib="NIH_MGC_57"
/lab_host="DH10B"
/note="Vector: pDNR-LIB"
gene 1..1220
/gene="PSMA4"
/gene_synonym="HC9"
/gene_synonym="HsT17706"
/gene_synonym="MGC12467"
/gene_synonym="MGC24813"
/db_xref="GeneID:5685"
/db_xref="HGNC:HGNC:9533"
CDS 235..948
/gene="PSMA4"
/gene_synonym="HC9"
/gene_synonym="HsT17706"
/gene_synonym="MGC12467"
/gene_synonym="MGC24813"
/codon_start=1
/transl_table=11
/product="PSMA4 protein"
/protein_id="AAH56249.1"
/db_xref="GeneID:5685"
/db_xref="HGNC:HGNC:9533"
/translation="MEAIGHAGTCLGILANDGVLLAAERRNIHKLLDEVFFSEKIYKL
NEDMACSVAGITSDANVLTNELRLIAQRYLLQYQEPIPCEQLVTALCDIKQAYTQFGG
KRPFGVSLLYIGWDKHYGFQLYQSDPSGNYGGWKATCIGNNSAAAVSMLKQDYKEGEM
TLKSALALAIKVLNKTMDVSKLSAEKVEIATLTRENGKTVIRVLKQKEVEQLIKKHEE
EEAKAEREKKEKEQKEKDK"
BASE COUNT 402 a 215 c 277 g 326 t
ORIGIN
1 aagcagtggt atcaacgcag agtgcccatt acggccgggg ggccatatta gcagcggtta
61 ttcggtgagc ggtggtggtt tattcttccg cggagttaag ggctccgtgg acatctcagg
121 tcttcagggt cttccatctg gaactatata aagttcagaa aacattctcg aagatatgac
181 tccaggacca ctatattttc tccagaaggt cgcttatacc aagttgaata tgccatggaa
241 gctattggac atgcaggcac ctgtttggga attttagcaa atgatggtgt tttgcttgca
301 gcagagagac gcaacatcca caagcttctt gatgaagtct ttttttctga aaaaatttat
361 aaactcaatg aggacatggc ttgcagtgtg gcaggcataa cttctgatgc taatgttctg
421 actaatgaac taaggctcat tgctcaaagg tatttattac agtatcagga gccaatacct
481 tgtgagcagt tggttacagc gctgtgtgat atcaaacaag cttatacaca atttggagga
541 aaacgtccct ttggtgtttc attgctgtac attggctggg ataagcacta tggctttcag
601 ctctatcaga gtgaccctag tggaaattac gggggatgga aggccacatg cattggaaat
661 aatagcgctg cagctgtgtc aatgttgaaa caagactata aagaaggaga aatgaccttg
721 aagtcagcac tcgctttagc tatcaaagta ctaaataaga ccatggatgt tagtaaactc
781 tctgctgaaa aagtggaaat tgcaacacta acaagagaga atggaaagac agtaatcaga
841 gttctcaaac aaaaagaagt ggagcagttg atcaaaaaac acgaggaaga agaagccaaa
901 gctgagcgtg agaagaaaga aaaagaacag aaagaaaagg ataaatagaa tcagagattt
961 tattactcat ttggggcacc atttcagtgt aaaagcagtc ctactcttcc acactaggaa
1021 ggctttactt tttttaactg gtgcagtggg aaaataggac gttacatact gaattgggtc
1081 cttgtcattt ctgtccaatt gaatacttta ttgtaacgat gatggttacc cttcatggac
1141 gtcttaatct tccacacaca tccccttttt tttggaataa aatttggaaa atggaaaaaa
1201 aaaaaaaaaa aaaaaaaaaa
//