LOCUS BC055415 1240 bp mRNA linear HUM 16-SEP-2003
DEFINITION Homo sapiens phospholipid scramblase 2, mRNA (cDNA clone
IMAGE:6617791), partial cds.
ACCESSION BC055415
VERSION BC055415.1
KEYWORDS .
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1240)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 1240)
AUTHORS Strausberg,R.
TITLE Direct Submission
JOURNAL Submitted (31-JUL-2003) National Institutes of Health, Mammalian
Gene Collection (MGC), Cancer Genomics Office, National Cancer
Institute, 31 Center Drive, Room 11A03, Bethesda, MD 20892-2590,
USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: CLONTECH
cDNA Library Preparation: CLONTECH Laboratories, Inc.
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: National Institutes of Health Intramural
Sequencing Center (NISC),
Gaithersburg, Maryland;
Web site: http://www.nisc.nih.gov/
Contact: nisc_mgc@nhgri.nih.gov
Akhter,N., Ayele,K., Beckstrom-Sternberg,S.M., Benjamin,B.,
Blakesley,R.W., Bouffard,G.G., Breen,K., Brinkley,C., Brooks,S.,
Dietrich,N.L., Granite,S., Guan,X., Gupta,J., Haghighi,P.,
Hansen,N., Ho,S.-L., Karlins,E., Kwong,P., Laric,P., Legaspi,R.,
Maduro,Q.L., Masiello,C., Maskeri,B., Mastrian,S.D.,McCloskey,J.C.,
McDowell,J., Pearson,R., Stantripop,S., Thomas,P.J., Touchman,J.W.,
Tsurgeon,C., Vogt,J.L., Walker,M.A., Wetherby,K.D., Wiggins,L.,
Young,A., Zhang,L.-H. and Green,E.D.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 48 Row: k Column: 18
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 9966794.
FEATURES Location/Qualifiers
source 1..1240
/db_xref="H-InvDB:HIT000259506"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="IMAGE:6617791"
/tissue_type="Testis"
/clone_lib="NIH_MGC_82"
/lab_host="DH10B"
/note="Vector: pDNR-LIB"
gene <1..1240
/gene="PLSCR2"
/db_xref="GeneID:57047"
/db_xref="MIM:607610"
CDS <1..955
/gene="PLSCR2"
/codon_start=2
/product="PLSCR2 protein"
/protein_id="AAH55415.1"
/db_xref="GeneID:57047"
/db_xref="MIM:607610"
/translation="GVSAHLRFFLAIILRDSGLWAEWRMKPFQIHLPGPPGHIVYPKH
QAGHTGKQADHLGSQAFYPGRQHDYLVPPAGTAGIPVQNQPGRPEGVPWMPAPPPPLN
CPPGLEYLSQIDMILIHQQIELLEVLFSFESSNMYEIKNSFGQRIYFAAEDTNFCIRN
CCGRSRPFTLRITDNVGREVITLERPLRCNCCCCPCCLQEIEIQAPPGVPVGYVTQTW
HPCLTKFTIKNQKREDVLKISGPCIVCSCIAGVDFEITSLDEQIVVGRISKHWSGFLR
EAFTDADNFGIQFPRDLDVKMKAVMIGACFLIDYMFFERTR"
misc_feature 317..946
/gene="PLSCR2"
/note="Scramblase; Region: Scramblase. Scramblase is
palmitoylated and contains a potential protein kinase C
phosphorylation site. Scramblase exhibits Ca2+-activated
phospholipid scrambling activity in vitro. There are also
possible SH3 and WW binding motifs. Scramblase is involved
in the redistribution of phospholipids after cell
activation or injury"
/db_xref="CDD:pfam03803"
BASE COUNT 390 a 244 c 262 g 344 t
ORIGIN
1 gggggtatct gcccatctta gattcttcct agcaataatt ctgagagact ctggattgtg
61 ggccgaatgg agaatgaagc cttttcaaat tcacctccca ggacctcccg gacatattgt
121 ctaccctaag caccaggctg gacacactgg gaaacaagct gaccacctgg gctcccaggc
181 cttctaccca ggacgtcagc atgactacct agtcccacct gctggcacag ctggcattcc
241 tgttcaaaat cagccaggta gacctgaagg ggtaccatgg atgccagcac caccaccacc
301 attaaactgt ccgccaggat tggaatactt aagtcagata gatatgatac taattcatca
361 gcaaattgaa cttctggaag ttctattcag ttttgaaagt agtaacatgt atgaaatcaa
421 gaacagcttt gggcagagga tttattttgc agcagaagat actaatttct gtatccgaaa
481 ttgctgtggg cggtctagac cttttacctt gaggattact gataatgtgg gtcgagaagt
541 cataactctg gaaagaccac taagatgtaa ctgttgttgt tgcccctgct gccttcagga
601 gatagaaatc caagctcctc ctggtgtacc agtaggttat gttactcaga cctggcaccc
661 atgtctaaca aagtttacaa ttaaaaatca gaaaagagag gatgtactaa aaattagtgg
721 tccatgtatc gtgtgcagct gtattgcggg tgttgatttt gagattacat ctcttgatga
781 acaaattgtg gttggcagga tttctaagca ctggtctggg tttttaagag aggcatttac
841 tgatgctgac aactttggaa tccaattccc tagagacctt gatgttaaaa tgaaagccgt
901 gatgattggt gcctgtttcc tcattgacta catgtttttt gaaagaacta ggtaatgact
961 ggaatgtcag agtgtgggag tggattaatg attccggatc tttggctagg caaaatgaaa
1021 ctataactga tctaaacggt tccttccttc ttctactgtg caaggaagat gtaaggaaaa
1081 actcgcacac tatctgtgga actcatttaa attcaaatcc tagataaaca tttcgcattg
1141 aatatttaca tggagaaaaa tcatcaaaca tcaacaatta tcaagttaat taataaaaat
1201 actaggtatt acaaaaaaaa aaaaaaaaaa aaaaaaaaaa
//